Chlamydia psittaci in turkeys: pathogenesis of infections in avian serovars A, B and D

Vanrompay, Daisy; Mast, Jan; Ducatelle, Richard; Haesebrouck, Freddy; Goddeeris, Bruno

doi:10.1016/0378-1135(95)00125-5

Cited by 44 publications

(19 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consequently, epithelial degeneration during intracellular C. psittaci replication may allow extensive E. coli colonisation of the respiratory tract. Moreover, defence mechanisms of macrophages might have been reduced by the C. psittaci infection of the macrophages themselves [13,27].…”

Section: Discussionmentioning

confidence: 99%

Exacerbation ofChlamydophila psittacipathogenicity in turkeys superinfected byEscherichia coli

et al. 2006

View full text Add to dashboard Cite

-Both Chlamydophila psittaci and Escherichia coli infections are highly prevalent in Belgian turkeys and therefore they both might contribute to the respiratory disease complex observed in turkeys. C. psittaci can infect turkeys within the first week of age, even in the presence of maternal antibodies. However, the first C. psittaci outbreaks occur mostly at the age of 3 to 6 weeks, the period when also E. coli infections appear on the farms. Therefore, we examined in this study the pathogenicity of an E. coli superinfection on C. psittaci predisposed turkeys. Turkeys were infected with C. psittaci, E. coli or with C. psittaci followed by E. coli. Simulating the impact of an E. coli infection during the acute phase or the latent phase of a C. psittaci infection, turkeys received E. coli at 1 or 5 weeks post C. psittaci infection, respectively. E. coli superinfection during the acute phase of C. psittaci infection increased C. psittaci excretion and stimulated chlamydial replication in the respiratory tract resulting in exacerbated clinical disease. Interestingly, E. coli superinfection during the latent phase of C. psittaci infection induced chlamydial replication, leading to increased C. psittaci-specific antibody titres. In addition, chlamydial predisposition gave higher E. coli excretion compared with turkeys that had only been infected with E. coli. Overall, the present study clearly demonstrates the pathogenic interplay between C. psittaci and E. coli resulting in more severe respiratory disease. Chlamydophila psittaci / Escherichia coli / turkeys

show abstract

Section: Discussionmentioning

confidence: 99%

Exacerbation ofChlamydophila psittacipathogenicity in turkeys superinfected byEscherichia coli

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Genotype E/B can only be detected using ompA sequencing since it generates the same restriction pattern as genotype E [9]. Genotypes A, B and D differ in pathogenicity for turkeys [28,29] but the pathogenic significance of avian genotypes for different host species, including man has to be investigated further. The presently developed genotype-specific realtime PCR, allowing the detection of the newly discovered E/B genotype by the use of an MGB probe, could contribute to this research.…”

Section: Discussionmentioning

confidence: 99%

Development of a Chlamydophila psittaci species-specific and genotype-specific real-time PCR

et al. 2005

View full text Add to dashboard Cite

-A Chlamydophila psittaci species-specific real-time PCR targeting the rDNA ribosomal spacer was developed as well as a genotype-specific real-time PCR targeting the Cp. psittaci outer membrane protein A (ompA) gene. The SYBR Green-based species-specific real-time PCR detected Cp. psittaci genotypes A to F, and the recently discovered E/B genotype. The genotype-specific real-time PCR could easily distinguish genotypes C, D, F by use of TaqMan probes. Genotypes A, B and E could not be distinguished from each other by simply using TaqMan probes. For this purpose, non-fluorescent competitor oligonucleotides, had to be used next to the TaqMan probes. Genotype E/B could only be detected by use of a minor groove binder (MGB) probe. Both real-time PCR assays allowed reproducible, sensitive (10 rDNA or ompA copies/µL DNA extract) and specific detection of Cp. psittaci DNA. The genotype-specific real-time PCR was compared to ompA sequencing and ompA restriction fragment length polymorphism (RFLP) analysis using five Cp. psittaci field isolates (99, 61/8, 7344/2, 8615/1 and 7778B15) each consisting of two different genotypes. The currently developed real-time PCR assays were used in a case study on a veterinary school and a turkey farm. In the veterinary school, Cp. psittaci genotypes D, E/B and F infection were detected in all five groups of turkeys, and one veterinarian who was taking care of all these turkeys. On the turkey farm, the presence of two Cp. psittaci genotype B infection waves was demonstrated in one randomly selected turkey, the first wave at the age of 6 weeks, and the second at the age of 12 weeks.

show abstract

“…Later, Chlamydia was found in large number in cloaca and nasal turbinate. Furthermore, Vanrompay et al [31] , from their experiment on pathogenesis of C. psittaci in turkey, reported that Chlamydaemia was observed in these turkey before chlamydial replication could be detected in the digestive tracts, 3-5 days post infection. The higher detection of Chlamydia in blood in this study supported the possibility of early detection of Chlamydia in birds before detection from feces or cloacal swabs.…”

Section: Discussionmentioning

confidence: 98%

İran’da Şehir Güvercinlerinde (Columba livia domestica) Kan, Karaciğer ve Kas Dokularında Chlamydophila psittaci’nin Moleküler Metotlarla Belirlenmesi ve Prevalansı

Khodadadi¹,

Hemmatinezhad²,

Doosti³

et al. 2015

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

Chlamydophila psittaci (C. psittaci) is a widespread pathogenic bacterium in pigeons. These animals are mostly infected without any clinical signs. Pigeons are probably the most commonly reported chlamydia-infected avian species. Shedding of Chlamydia from infected birds has been widely reported. This study was conducted to detect and to determine the prevalence of C. psittaci in the blood, liver and muscle tissue of urban pigeons in Iran using conventional polymerase chain reaction. In this study, authors used 90 pigeons from different retail shops across Iran. The study was including 26 female and 64 male pigeons with suspected Chlamydiosis based on clinical signs. During examination of the corpses we took 270 samples in total, including blood, liver and muscle tissue from each animal. C. psittaci was detected in 16 (17.78%) blood samples, 14 (15.56%) liver samples and 5 (5.56%) samples of muscle tissue. This study supports the fact that pigeons serve as carriers of C. psittaci. Therefore, continuous surveillance of this bacterium will go along way in understanding the distribution and risks associated with Chlamydia infected pigeons. This will be beneficial in prevention and control risks of infection in humans. Keywords

show abstract

Chlamydia psittaci in turkeys: pathogenesis of infections in avian serovars A, B and D

Cited by 44 publications

References 11 publications

Exacerbation ofChlamydophila psittacipathogenicity in turkeys superinfected byEscherichia coli

Exacerbation ofChlamydophila psittacipathogenicity in turkeys superinfected byEscherichia coli

Development of a Chlamydophila psittaci species-specific and genotype-specific real-time PCR

İran’da Şehir Güvercinlerinde (Columba livia domestica) Kan, Karaciğer ve Kas Dokularında Chlamydophila psittaci’nin Moleküler Metotlarla Belirlenmesi ve Prevalansı

Contact Info

Product

Resources

About