Chlamydia pneumoniae in coronary bypass grafts of redo patients. The concept of the ‘adventitial baseline infection’

Glasz, Tibor; Hortoványi, Eszter; Mózes, Géza; Kiss, András; Lotz, Gábor; Nagy, Péter; Simonyi, Ágnes; Kardos, Magdolna; Sziller, István; Nagy, Bálint; Bán, Zoltán; Tóth, András; Kassai, Imre; Horkay, Ferenc; Dudás, Gábor; Kádár, Anna

doi:10.1016/j.prp.2003.08.001

Cited by 2 publications

(2 citation statements)

References 28 publications

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“…This partly accounts for the accelerated formation of SVG atherosclerosis. 65,66 As time passes, dense fi brous tissue is increased proportionally so that venous lesions resemble those of native coronary atherosclerosis. 60 Recently, certain infectious agents have been proposed to play a role in atherosclerosis in both arteries and vein grafts; most studies in vein grafts have focused on the presence of Chlamydia pneumoniae in atherosclerotic plaques.…”

Section: Midterm Graft Failurementioning

confidence: 99%

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Pathophysiology of Aortocoronary Saphenous Vein Bypass Graft Disease

Hassantash¹,

Bikdeli²,

Kalantarian³

et al. 2008

Asian Cardiovasc Thorac Ann

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Aortocoronary saphenous vein bypass grafting relieves anginal pain in patients with coronary artery disease. However, its effectiveness is limited due to graft failure; the 10-year patency rate is 50%-60%. Early, 1-year and late graft failure may be due to thrombosis, fibrointimal hyperplasia and atherosclerosis, respectively. There is general agreement that vein graft atherosclerosis differs from arterial lesions in terms of temporal and histological changes. Vein graft atherosclerosis is more rapid, with diffuse concentric changes and a less noticeable fibrous cap, making venous plaques more vulnerable to rupture and subsequent thrombus formation. Despite progress in understanding the pathophysiology, some aspects of vein graft atherosclerosis need to be clarified. This review focuses on the pathophysiologic aspects of this widespread, costly and disabling disease, with emphasis on late graft occlusion and distinctions between arterial and venous atherosclerosis in terms of histology, pathophysiology and risk factors.

show abstract

Section: Midterm Graft Failurementioning

confidence: 99%

“…60 Recently, certain infectious agents have been proposed to play a role in atherosclerosis in both arteries and vein grafts; most studies in vein grafts have focused on the presence of Chlamydia pneumoniae in atherosclerotic plaques. [65][66][67] A role for other infectious agents such as Helicobacter pylori remains elusive.…”

Section: Midterm Graft Failurementioning

confidence: 99%

Pathophysiology of Aortocoronary Saphenous Vein Bypass Graft Disease

Hassantash¹,

Bikdeli²,

Kalantarian³

et al. 2008

Asian Cardiovasc Thorac Ann

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Az emberi májrák komparatív genomikai osztályozása

Kaposi-Novák

2009

Magyar Onkológia

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Global transcriptome analysis has been successfully applied to characterize various human tumors, including hepatocellular carcinomas. This novel technology can facilitate early diagnosis, as well as prognostic and therapeutic diversification of cancer patients. To enhance access to the genomic information buried in archived pathology samples, we assessed RT-PCR amplification rates in paraffin-embedded tissues preserved in three different fixatives. Reliable amplification could be achieved from all paraffin-embedded specimens, when the amplicon size did not exceed 225 bp. A longer amplicon size resulted in rapid decrease of yield and reproducibility. In addition, formalin provided superior morphology and better reactivity with claudin-4 and -7 immunohistochemistry. Amplification of the initial sample is often required before transcriptome analysis of clinical specimens could be performed. We introduced a random nonamer primed T3 polymerase reaction into the conventional linear RNA amplification protocol. The modified T3T7 method generated a sense strand product ideal for synthesizing indirectly labeled cDNA templates. Microarray analysis of amplified frozen and laser-microdissected Myc and Myc/TGFalpha mouse liver tumors confirmed good reproducibility (r=0.9) of the reaction and conservation of original transcriptional patterns (r=0.78). Finally, we tested the utility of expression profiling for the classification of human HCC samples. By comparing expression data from HGF-treated c-Met conditional knock-out and control primary mouse hepatocytes, we identified 690 HGF/c-Met target genes. Functional analysis of the significant gene set implicated c-Met as key regulator of hepatocyte motility and oxidative homeostasis. Cross comparison of the c-Met-induced transcription signature with human HCC expression profiles revealed a group of tumors (27%) with potentially activated c-Met signaling (MET+). These tumors were characterized by higher vascular invasion rate, increased microvessel density, and shortened survival. A prediction model based on 111 cross-species conserved c-Met signature genes was able to diversify HCC patients into good and bad prognostic groups with 83-95% accuracy. Our results therefore demonstrate that careful experimental design and state-of-the-art laboratory methods could open the way for global expression profiling of archived and limited availability pathologic samples. Comparative functional genomics based analysis of the cancer transcriptome could lead to novel molecular classification systems which are essential for the introduction of individualized cancer therapeutics.

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Chlamydia pneumoniae in coronary bypass grafts of redo patients. The concept of the ‘adventitial baseline infection’

Cited by 2 publications

References 28 publications

Pathophysiology of Aortocoronary Saphenous Vein Bypass Graft Disease

Pathophysiology of Aortocoronary Saphenous Vein Bypass Graft Disease

Az emberi májrák komparatív genomikai osztályozása

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