Chitosomes as drug delivery systems for C-phycocyanin: Preparation and characterization

Manconi, Maria; Mura, Simona; Manca, Maria Letizia; Fadda, Anna Maria; Dolz, M.; Hernández, M. J.; Casanovas, A.; Dı́ez-Sales, Octavio

doi:10.1016/j.ijpharm.2010.03.038

Cited by 68 publications

(41 citation statements)

References 36 publications

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“…PAM and PACM both showed a slower release rate in the acidic medium, as depicted in Fig. 5, similar to the results reported by others (Manconi et al, 2010;Yoo et al, 2006). When they were transferred into SIF, dramatic increases were observed in release percentage of phycocyanin initially, thereafter slight decreases were found, which was ascribed to proteases in SGF and SIF.…”

Section: In Vitro Phycocyanin Releasing Property Of Microcapsulessupporting

confidence: 89%

Preparation of phycocyanin microcapsules and its properties

Yan

Liu

Jiao

et al. 2014

Food and Bioproducts Processing

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a b s t r a c tPhycocyanin was microencapsulated by an extrusion method using alginate and chitosan as coating materials. This work was aimed to optimize the encapsulation process, characterize the physicochemical properties of microcapsules, and evaluate the storage stability and in vitro release performance. The optimum process conditions for preparing microcapsule gained from the single factor experiments were as follows: alginate content 2.5%, ratio of phycocyanin to alginate 1.5:1, content of calcium chloride 2.5%, and chitosan content 2.0%. Phycocyanin/alginate/chitosan microcapsules (PACM) were found to have compact spherical shape with mean diameters of 1.03 mm, whereas phycocyanin/alginate microspheres (PAM) were internal porous spherical appearances with mean diameters of 1.81 mm. Storage stability study showed that encapsulation by alginate and chitosan conferred greater ability to phycocyanin against temperature during storage. In vitro release study revealed that both PAM and PACM could be resistant against acidic environment, and would rapidly release phycocyanin under mild alkali condition. The sustained-release profile of phycocyanin from PACM was superior to that from PAM.© 2013 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.Keywords: Microcapsule; Phycocyanin; Alginate; Chitosan IntroductionPhycocyanin is a blue phycobiliprotein, composed of two relatively homologous subunits: the ␣-chain with one phycocyanobilin attached at cysteine 84 and the ␤-chain with two phycocyanobilins attached at cysteines 84 and 155. The two subunits form ␣␤ monomers, which aggregate into ␣ 3 ␤ 3 trimers and further into disc-shaped ␣ 6 ␤ 6 hexamers, the functional unit of phycocyanin (Eriksen, 2008). It has good therapeutic values, such as antioxidative, immunomodulating, anti-cancer, antiviral, anti-allergic, anti-mutagenic, anti-inflammatory, hepatoprotective, blood vessel-relaxing and blood lipid-lowering activities (Thangam et al., 2013), which made it a better active ingredient in functional food. It is also used as natural dyes in food including chewing gum, ice sherbets, soft drinks, and candies, and cosmetics including lipstick and eyeliners, replacing the synthetic colourants (Chaiklahan et al., 2011). Another application of phycocyanin is as phycofluor probes for immunodiagnosis owing to its fluorescence properties. However, its application is often limited by the instability towards moisture, light and temperature due to the degradation of the protein fraction (Chaiklahan * Corresponding author. , 2012). Studies show that microencapsulation is an effective and economical method for protecting natural colourants against adverse conditions (Rocha et al., 2012). Therefore, it was inferred that the stability of phycocyanin should be improved using microencapsulation technologies. However, up to now, reports were scarce about phycocyanin microencapsulation. Microencapsulation is a technique by which the sensitive ingredients, called core materials, are entrapped in coati...

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Section: In Vitro Phycocyanin Releasing Property Of Microcapsulessupporting

confidence: 89%

Preparation of phycocyanin microcapsules and its properties

Yan

Liu

Jiao

et al. 2014

Food and Bioproducts Processing

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“…Chitosan is a polymer well known for its attractive properties, such as biocompatibility, biodegradability and bioadhesivity (Gulbake and Jain, 2012). Chitosomes maintained the positive properties of liposomes, but showed greater stability and provided longer residence times in the gastrointestinal tract, in virtue of their mucoadhesion properties (Takeuchi et al, 2003(Takeuchi et al, , 2005Werle and Takeuchi, 2009;Manconi et al, 2010Manconi et al, , 2013Shao et al, 2015;Caddeo et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Calcium alginate microspheres containing metformin hydrochloride niosomes and chitosomes aimed for oral therapy of type 2 diabetes mellitus

Maestrelli

Mura

González-Rodrı́guez

et al. 2017

International Journal of Pharmaceutics

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“…Chitosan-coated liposomes (so-called chitosomes) were previously prepared by several authors and their effective formation was confirmed using several techniques such as SEM and TEM (11,14,(41)(42)(43).…”

Section: Preparation and Characterizationmentioning

confidence: 99%

“…Mucoadhesive polymers have also been used to protect and stabilize liposomes by coating (8)(9)(10)(11). Among the various employed polymers, chitosan (CH) has been shown to possess good mucoadhesive properties in the hydrated form.…”

Section: Introductionmentioning

confidence: 99%

“…Among the various employed polymers, chitosan (CH) has been shown to possess good mucoadhesive properties in the hydrated form. Moreover, it has already successfully used to coat liposome formulations (10)(11)(12)(13)(14). Surface modification of liposomes with CH has been shown to prolong their residence time in the gastrointestinal tract of rats in comparison with uncoated ones, thanks to ionic interactions between positive amino groups of the polymer and the negative mucus gel layer (11,15,16).…”

Section: Introductionmentioning

confidence: 99%

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Improving Oral Bioavailability and Pharmacokinetics of Liposomal Metformin by Glycerolphosphate–Chitosan Microcomplexation

et al. 2013

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Abstract. The purpose of this study was to develop a new delivery system capable of improving bioavailability and controlling release of hydrophilic drugs. Metformin-loaded liposomes were prepared and to improve their stability surface was coated with chitosan cross-linked with the biocompatible β-glycerolphosphate. X-ray diffraction, differential scanning calorimetry, as well as rheological analysis were performed to investigate interactions between chitosan and β-glycerolphosphate molecules. The entrapment of liposomes into the chitosan-β-glycerolphosphate network was assessed by scanning electron microscopy and transmission electron microscopy. Swelling and mucoadhesive properties as well as drug release were evaluated in vitro while the drug oral bioavailability was evaluated in vivo on Wistar rats. Results clearly showed that, compared to control, the proposed microcomplexes led to a 2.5-fold increase of metformin T max with a 40% augmentation of the AUC/D value.

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Chitosomes as drug delivery systems for C-phycocyanin: Preparation and characterization

Cited by 68 publications

References 36 publications

Preparation of phycocyanin microcapsules and its properties

Preparation of phycocyanin microcapsules and its properties

Calcium alginate microspheres containing metformin hydrochloride niosomes and chitosomes aimed for oral therapy of type 2 diabetes mellitus

Improving Oral Bioavailability and Pharmacokinetics of Liposomal Metformin by Glycerolphosphate–Chitosan Microcomplexation

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