Chitosanase purified from bacterial isolate Bacillus licheniformis of ruined vegetables displays broad spectrum biofilm inhibition

Muslim, Sahira Nsayef; Al-Kadmy, Israa M.S.; Hussein, Nadheema Hammood; Ali, Alaa Naseer Mohammed; Taha, Buthainah Mohammed; Aziz, Sarah Naji; Kheraif, Abdulaziz A. Al; Divakar, Darshan Devang; Ramakrishnaiah, Ravikumar

doi:10.1016/j.micpath.2016.10.001

Cited by 20 publications

(12 citation statements)

References 19 publications

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“…Chitosanase synthesized by B. licheniformis inhibited the biofilm formation of Pseudomonas aeruginosa and Klebsiella pneumoniae to about 22 and 29%, respectively, relative to a 100% growth in control samples. Thus, chitosanases have a promising advantage as an antibiotic agent, since they combat against biofilms formed by pathogenic bacteria ( Muslim et al, 2016 ). An antimicrobial agent (protein BL-DZ1 with a molecular weight of 14 kDa) isolated from a tropical marine strain of B. licheniformis acts as a potential antibiofilm agent ( Dusane et al, 2013 ).…”

Section: The Use Of Microbial Enzymes In Medicinementioning

confidence: 99%

The Practical Potential of Bacilli and Their Enzymes for Industrial Production

Danilova

Шарипова

2020

Front. Microbiol.

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Bacillus spp. are an affordable source of enzymes due to their wide distribution, safety in work, ease of cultivation, and susceptibility to genetic transformations. Researchers are particularly interested in proteolytic enzymes, which constitute one of the most diverse groups of microbial proteins in terms of properties. Despite the long history of their research, this group of enzymes continue to show great potential for practical application in the biomedical industry, as well as in the agricultural industry. Thus, the unique properties of bacillary proteinases, such as stability in a wide range of temperatures and pH, high specificity, biodegradability of a wide range of substrates, and the high potential of sequenced Bacillus genomes are a powerful foundation for the development of new biotechnologies. The current review aims to discuss recent studies on various enzymes in particular, proteinases produced by bacteria of the genus Bacillus , along with their prospective practical applications. This article also presents an interpretive summary of the recent developments on the usage of probiotic Bacillus strains as potential feed additives.

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Section: The Use Of Microbial Enzymes In Medicinementioning

confidence: 99%

The Practical Potential of Bacilli and Their Enzymes for Industrial Production

Danilova

Шарипова

2020

Front. Microbiol.

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“…Amino acid sequence homologies of chitosanases verified them as a member of glycoside hydrolase families 5, 7, 8, 46, 75, and 80 in the Carbohydrate Active Enzyme database (CAZy) . Among bacterial genera, Bacillus , Pseudomonas , Myxobacter , Enterobacter , Streptomyces , and Nocardia were previously described to possess chitosanase producing strains . Chitosanases have various industrial as well as biotechnological applications such as, synthesis of COS from chitosan, bioconversion, or valorization of seafood processing chitionous biowaste, hydrolysis of chitosan containing fungal cell wall for protoplast preparation, chitosan mediated gene delivery to improves gene expression by endo‐cellular chitosan degradation, biocontrol of phytopathogens, and development of transgenic plants .…”

Section: Introductionmentioning

confidence: 98%

“…Chitosans with a degree of polymerization (DP) less than 20 and an average molecular weight less than 3900 Da are called chitooligosaccharides (COS) . Chitosanases [EC3.2.1.132] are chitosan hydrolysing enzymes targeting β‐1,4‐glycosidic linkage to yield COS . Chitosanases have been discovered from various organisms, including bacteria, fungi, viruses, and plants.…”

Section: Introductionmentioning

confidence: 99%

Optimization of chitosanase production by Bacillus mojavensis EGE‐B‐5.2i

et al. 2018

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Maximum production of industrially important enzymes such as chitosanases through media optimization still holds foremost interest. The present study was conducted to improve chitosanase activity of an indigenous strain identified as Bacillus mojavensis. Initially, carbon and nitrogen sources were optimized by one-variable-at-a-time approach. Further, fermentation medium was optimized using Plackett-Burman (PB) and central composite designs (CCD). PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production. CCD results predicted the optimum concentrations of SS, CC, peptone, and NaCl as 7.8, 7.0, 6.5, and 2.7 g L , respectively to achieve maximum chitosanase activity (21.1 U ml ). Discovery of the novel optimal medium has improved chitosanase production by B. mojavensis up-to 9.5 folds. Lastly, 18.6 U ml chitosanase activity was achieved in stirred tank bioreactor using optimal medium, which is quite satisfactory to proclaim this strain as a potential candidate to provide commercial chitosanase.

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“…The secreted primary and secondary metabolites from some gut microbiota can inhibit the bacterial infections or even nourish the hosts . Bacillus licheniformis ( B. licheniformis ) is a kind of rod‐sharp bacterium commonly found in the soil or bird feathers . Widely used as a probiotic, B. licheniformis is applied in the treatment of diarrhea to suppress the growth of pathologic bacteria .…”

Section: Introductionmentioning

confidence: 99%

“…The secreted primary and secondary metabolites from some gut microbiota can inhibit the bacterial infections or even nourish the hosts [2,3]. Bacillus licheniformis (B. licheniformis) is a kind of rod-sharp bacterium commonly found in the soil or bird feathers [4,5]. Widely used as a probiotic, B. licheniformis is Abbreviations: AA, amino acid; Ala-Gln, L-alanyl-L-glutamine; B. licheniformis, Bacillus licheniformis; FITC, fluorescein isothiocyanate; Gly, glycine; HSCE, high-speed capillary electrophoresis; HSCMEKC, high-speed capillary micellar electrokinetic chromatography; MFC, microbial fuel cell; Trp, Tryptophan; Tyr, tyrosine; Val, valine Conflict of interest: The authors have declared no conflict of interest.…”

Section: Introductionmentioning

confidence: 99%

Separation and determination of peptide metabolite of Bacillus licheniformis in a microbial fuel cell by high-speed capillary micellar electrokinetic chromatography

et al. 2017

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A method using high-speed capillary micellar electrokinetic chromatography and a microbial fuel cell was applied to determine the metabolite of the peptides released by Bacillus licheniformis. Two peptides, l-carnosine and l-alanyl-l-glutamine were used as the substrate to feed Bacillus licheniformis in a microbial fuel cell. The metabolism process of the bacterium was monitored by analyzing the voltage outputs of the microbial fuel cell. A home-made spontaneous injection device was applied to perform high-speed capillary micellar electrokinetic chromatography. Under the optimized conditions, tryptophan, glycine, valine, tyrosine and the two peptides could be rapidly separated within 2.5 min with micellar electrokinetic chromatography mode. Then the method was applied to analyze the solutions sampled from the microbial fuel cell. After 92 h running, valine, as the metabolite, was successfully detected with concentration 3.90 × 10 M. The results demonstrated that Bacillus licheniformis could convert l-carnosine and l-alanyl-l-glutamine into valine. The method employed in this work was proved to have great potential in analysis of metabolites, such as amino acids, for microorganisms.

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Chitosanase purified from bacterial isolate Bacillus licheniformis of ruined vegetables displays broad spectrum biofilm inhibition

Cited by 20 publications

References 19 publications

The Practical Potential of Bacilli and Their Enzymes for Industrial Production

The Practical Potential of Bacilli and Their Enzymes for Industrial Production

Optimization of chitosanase production by Bacillus mojavensis EGE‐B‐5.2i

Separation and determination of peptide metabolite of Bacillus licheniformis in a microbial fuel cell by high-speed capillary micellar electrokinetic chromatography

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