Chitosanase displayed on liposome can increase its activity and stability

Ngo, Kien Xuan; Umakoshi, Hiroshi; Shimanouchi, Toshinori; Sugaya, Hiroyuki; Kuboi, Ryoichi

doi:10.1016/j.jbiotec.2010.01.014

Cited by 8 publications

(4 citation statements)

References 41 publications

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“…The above Off-On effect of DOTAP liposome and oxidative stress could be investigated based on DOTAP liposome's recognition of the GFP mRNA on its surface. Based on phenomena related to the up-regulation of target product under stress conditions i n vivo 16, 17, 18, 19, 20 and in vitro, 28, 29, 30 gene expression can usually be achieved by modulation of the possible recognition of biomolecules by the “(stressed) biomembrane” as described in our series of reports. If the DOTAP liposome-mRNA complex approached to the cancer cell generating oxidative stress, the target gene will be expressed through the regulation of the liposome mRNA interaction.…”

Section: Resultsmentioning

confidence: 99%

Oxidative Stress Can Affect the Gene Silencing Effect of DOTAP Liposome in an In Vitro Translation System

Umakoshi¹,

Tanabe

Suga

et al. 2011

Int. J. Biol. Sci.

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Oxidative stress can affect in vitro GFP expression through its control of the gene silencing effect of the liposome prepared by 1,2-dioleoyl-3-trimethyl-ammonium propane (DOTAP). The gene silencing effect of cationic DOTAP liposome in in vitro GFP expression, especially focusing on its translation process, and the effects of oxidative stress on its silencing effect were investigated. GFP expression, initiated by mRNA, was found to be thoroughly inhibited in the presence of DOTAP liposome at concentration of more than 2.5 mM, though its inhibitory effect was reduced in the presence of hydrogen peroxide. The analyses of (i) the interaction of mRNA with DOTAP, (ii) the chemical structure of DOTAP, and (iii) the membrane fluidity of DOTAP liposome imply the possible role of gene expression by the liposome membrane and stress conditions.

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Section: Resultsmentioning

confidence: 99%

Oxidative Stress Can Affect the Gene Silencing Effect of DOTAP Liposome in an In Vitro Translation System

Umakoshi¹,

Tanabe

Suga

et al. 2011

Int. J. Biol. Sci.

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“…Enzymes, peptides, and nanoparticles may now be encapsulated within liposomes thanks to advances in entrapment techniques. Encapsulation techniques preserve enzyme function by preserving it from denaturation caused by pH extremes, high temperatures, and protease action (Ngo et al, 2010).…”

Section: Liposomesmentioning

confidence: 99%

No Solid Colloidal Carriers: Aspects Thermodynamic the Immobilization Chitinase and Laminarinase in Liposome

Alonso-Estrada

Ochoa-Viñals

Pacios‐Michelena

et al. 2022

Front. Bioeng. Biotechnol.

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The present review describes the basic properties of colloidal and vesicular vehicles that can be used for immobilization of enzymes. The thermodynamic aspects of the immobilization of enzymes (laminarinase and chitinase) in liposomes are discussed. These systems protect enzymes against environmental stress and allow for a controlled and targeted release. The diversity of colloidal and vesicular carriers allows the use of enzymes for different purposes, such as mycolytic enzymes used to control phytopathogenic fungi.

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“…The linkage effects of membranes on the aggregation of translocating polypeptides have not been considered in most studies of de novo protein folding. The cell surface display technology allows protein of interest on the membranes or cell surfaces by fusing them to surface anchoring motifs [ 45 , 46 , 59 – 61 ]. The popular anchoring motifs for the E. coli outer membrane display include porins, lipoproteins, glycosyl-phophatidylinositol anchor, and β-autotransporters [ 60 ].…”

Section: Macromolecule Linkage-mediated Folding Helper Systems mentioning

confidence: 99%

“…The popular anchoring motifs for the E. coli outer membrane display include porins, lipoproteins, glycosyl-phophatidylinositol anchor, and β-autotransporters [ 60 ]. Importantly, the display of proteins to the cell (or membrane) surfaces greatly stabilizes their linked proteins [ 45 , 46 , 62 ]. Similarly, the immobilization of proteins on the surfaces of beads is known to be a powerful method for stabilizing proteins [ 63 , 64 ].…”

Section: Macromolecule Linkage-mediated Folding Helper Systems mentioning

confidence: 99%

Macromolecule-Assisted de novo Protein Folding

Choi

Son

Lim

et al. 2012

IJMS

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In the processes of protein synthesis and folding, newly synthesized polypeptides are tightly connected to the macromolecules, such as ribosomes, lipid bilayers, or cotranslationally folded domains in multidomain proteins, representing a hallmark of de novo protein folding environments in vivo. Such linkage effects on the aggregation of endogenous polypeptides have been largely neglected, although all these macromolecules have been known to effectively and robustly solubilize their linked heterologous proteins in fusion or display technology. Thus, their roles in the aggregation of linked endogenous polypeptides need to be elucidated and incorporated into the mechanisms of de novo protein folding in vivo. In the classic hydrophobic interaction-based stabilizing mechanism underlying the molecular chaperone-assisted protein folding, it has been assumed that the macromolecules connected through a simple linkage without hydrophobic interactions and conformational changes would make no effect on the aggregation of their linked polypeptide chains. However, an increasing line of evidence indicates that the intrinsic properties of soluble macromolecules, especially their surface charges and excluded volume, could be important and universal factors for stabilizing their linked polypeptides against aggregation. Taken together, these macromolecules could act as folding helpers by keeping their linked nascent chains in a folding-competent state. The folding assistance provided by these macromolecules in the linkage context would give new insights into de novo protein folding inside the cell.

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Chitosanase displayed on liposome can increase its activity and stability

Cited by 8 publications

References 41 publications

Oxidative Stress Can Affect the Gene Silencing Effect of DOTAP Liposome in an In Vitro Translation System

Oxidative Stress Can Affect the Gene Silencing Effect of DOTAP Liposome in an In Vitro Translation System

No Solid Colloidal Carriers: Aspects Thermodynamic the Immobilization Chitinase and Laminarinase in Liposome

Macromolecule-Assisted de novo Protein Folding

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