Properties of gelatin/chitosan blend films plasticised with different plasticisers and incorporated with liposomes loaded with betel leaf ethanolic extract (L/BLEE) at 2% or BLEE at an equivalent amount were investigated. Films plasticised with glycerol (GLY) and incorporated with BLEE (B-GLY) showed better properties (P < 0.05) than other films (F-GLY, LB-GLY, F-PEG, B,PEG and LB-PEG). Type of plasticiser had no effect on antioxidant activities of films (P > 0.05). SEM images of the films revealed homogenous surface and cross-section in film samples devoid of active compounds, while those incorporated with BLEE or L/BLEE had rougher surfaces and cross-sections. During storage at room temperature, lower oxidation took place in shrimp oil packaged in pouches made from gelatin/chitosan blend plasticised with GLY and incorporated with BLEE (B-GLY) compared with that packaged in lower density polyethylene (LDPE) pouches. Therefore, B-GLY films could be used as active packaging to retard lipid oxidation in shrimp oil.