Chitosan sponges as tissue engineering scaffolds for bone formation

Seol, Yang‐Jo; Lee, Jue Yeon; Park, Yoon Jeong; Lee, Yong-Moo; Ku, Young; Rhyu, In‐Chul; Lee, Seung Jin; Han, Soo Boo; Chung, Chong Pyoung

doi:10.1023/b:bile.0000032962.79531.fd

Cited by 302 publications

(134 citation statements)

References 12 publications

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“…[53][54][55][56] The majority of these studies do not show mineralization data from cell-free controls. As seen in this study, although chitosan is clearly highly biocompatible and osteoconductive, 40,57,58 the osteoinductive potential of this particular ionotropic biomaterial should not be evaluated only by the calcium deposition.…”

Section: +mentioning

confidence: 99%

Fabrication and characterization of a rapid prototyped tissue engineering scaffold with embedded multicomponent matrix for controlled drug release

Chen

Le²,

Hein

et al. 2012

IJN

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Abstract:Bone tissue engineering implants with sustained local drug delivery provide an opportunity for better postoperative care for bone tumor patients because these implants offer sustained drug release at the tumor site and reduce systemic side effects. A rapid prototyped macroporous polycaprolactone scaffold was embedded with a porous matrix composed of chitosan, nanoclay, and β-tricalcium phosphate by freeze-drying. This composite scaffold was evaluated on its ability to deliver an anthracycline antibiotic and to promote formation of mineralized matrix in vitro. Scanning electronic microscopy, confocal imaging, and DNA quantification confirmed that immortalized human bone marrow-derived mesenchymal stem cells (hMSC-TERT) cultured in the scaffold showed high cell viability and growth, and good cell infiltration to the pores of the scaffold. Alkaline phosphatase activity and osteocalcin staining showed that the scaffold was osteoinductive. The drug-release kinetics was investigated by loading doxorubicin into the scaffold. The scaffolds comprising nanoclay released up to 45% of the drug for up to 2 months, while the scaffold without nanoclay released 95% of the drug within 4 days. Therefore, this scaffold can fulfill the requirements for both bone tissue engineering and local sustained release of an anticancer drug in vitro. These results suggest that the scaffold can be used clinically in reconstructive surgery after bone tumor resection. Moreover, by changing the composition and amount of individual components, the scaffold can find application in other tissue engineering areas that need local sustained release of drug.

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Section: +mentioning

confidence: 99%

Fabrication and characterization of a rapid prototyped tissue engineering scaffold with embedded multicomponent matrix for controlled drug release

Chen

Le²,

Hein

et al. 2012

IJN

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“…From a tissue-engineering perspective, induction of an antiinflammatory pro-regenerative macrophage phenotype is of key relevance, as reparative macrophages are crucial in tissue remodeling after inflammation, guiding the host response to implanted biomaterials. Thus, the claim that Ch may be an adequate material for tissue-engineering applications, such as in bone repair, 11,50 may be related to its ability to modulate the inflammatory response. Moreover, besides supporting attachment and proliferation of osteoblastic cells, 16,51 Ch also drives macrophage production of soluble mediators involved in bone physiology.…”

Section: Discussionmentioning

confidence: 99%

Adsorbed Fibrinogen Enhances Production of Bone- and Angiogenic-Related Factors by Monocytes/Macrophages

Maciel

Oliveira²,

Colton

et al. 2014

Tissue Engineering Part A

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Macrophages are phagocytic cells with great importance in guiding multiple stages of inflammation and tissue repair. By producing a large number of biologically active molecules, they can affect the behavior of other cells and events, such as the foreign body response and angiogenesis. Since protein adsorption to biomaterials is crucial for the inflammatory process, we addressed the ability of the pro-inflammatory molecule fibrinogen (Fg) to modulate macrophage behavior toward tissue repair/regeneration. For this purpose, we used chitosan (Ch) as a substrate for Fg adsorption. Freshly isolated human monocytes were seeded on Ch substrates alone or previously adsorbed with Fg, and allowed to differentiate into macrophages for 10 days. Cell adhesion and morphology, formation of foreign body giant cells (FBGC), and secretion of a total of 80 cytokines and growth factors were evaluated. Both substrates showed similar numbers of adherent macrophages along differentiation as compared with RGD-coated surfaces, which were used as positive controls. Fg did not potentiate FBGC formation. In addition, actin cytoskeleton staining revealed the presence of punctuate F-actin with more elongated and interconnecting cells on Ch substrates. Antibody array screening and quantification of inflammation-and wound-healing-related factors indicated an overall reduction in Ch-based substrates versus RGD-coated surfaces. At late times, most inflammatory agents were downregulated in the presence of Fg, in contrast to growth factor production, which was stimulated by Fg. Importantly, on Ch + Fg substrates, fully differentiated macrophages produced significant amounts of macrophage inflammatory protein-1delta (MIP-1d), platelet-derived growth factor-BB, bone morphogenetic protein (BMP)-5, and BMP-7 compared with Ch alone. In addition, other important factors involved in bone homeostasis and wound healing, such as growth hormone, transforming growth factor-b3, and insulin-like growth factor-binding proteins, as well as several angiogenic mediators, including endocrine gland-derived vascular endothelial factor, fibroblast growth factor-7, and placental growth factor, were significantly promoted by Fg. This work provides a new perspective on the inflammatory response in the context of bone repair/regeneration mediated by a pro-inflammatory protein (Fg) adsorbed onto a biomaterial (Ch) that does not otherwise exhibit osteogenic properties.

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“…Furthermore, chitosan membrane (10), nanofiber (11), and sponge (12) have been produced as tissue-engineering scaffolds for bone formation. However, chitin (chitosan) changes its physical and biological properties according to its deacetylation level (13).When mouse osteoblast cells were cultured on sheets made of nondeacetylated chitin, and 35, 50, and 70% deacetylated chitin (DAC-0, DAC-35, DAC-50, and DAC-70, respectively), DAC-35 had the highest effect of growth and differentiation of the cells, and DAC-50 and DAC-0 had intermediate effects, while DAC-70 suppressed the growth and differentiation of the cells (13).…”

Section: Discussionmentioning

confidence: 99%

“…The effects of rotary blood pumps on coronary blood flow have not been adequately documented; one study showed a decrease in the coronary flow in pigs with and without a surgically created coronary stenosis (11). Improved perfusion has been previously registered with the use of pulsatile pump systems (12). New information about coronary perfusion and myocardial oxygen consumption is THOUGHTS AND PROGRESS 77…”

Section: Coronary Hemodynamics and Myocardialmentioning

confidence: 99%

Photocrosslinkable Chitosan Hydrogel Can Prevent Bone Formation in Both Rat Skull and Fibula Bone Defects

et al. 2009

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UV light irradiation to a photocrosslinkable chitosan (Az-CH-LA) resulted in an insoluble and flexible hydrogel within 30 s. The purpose of this study was to evaluate the ability of the photocrosslinkable chitosan to inhibit bone formation in the bone defects. A 5-mmdiameter defect was made in the rat calvarium, and then photocrosslinkable chitosan was implanted and irradiated with UV for 30 s. Furthermore, a 2-mm defect was made in the fibula of a rat hind leg, and then photocrosslinkable chitosan was implanted and irradiated with UV. Bone formations in the rat skull and fibula defects with photocrosslinkable chitosan hydrogel were significantly prevented for 8 weeks. Thus, the chitosan hydrogel has an inhibitory effect on bone formation.

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Chitosan sponges as tissue engineering scaffolds for bone formation

Cited by 302 publications

References 12 publications

Fabrication and characterization of a rapid prototyped tissue engineering scaffold with embedded multicomponent matrix for controlled drug release

Fabrication and characterization of a rapid prototyped tissue engineering scaffold with embedded multicomponent matrix for controlled drug release

Adsorbed Fibrinogen Enhances Production of Bone- and Angiogenic-Related Factors by Monocytes/Macrophages

Photocrosslinkable Chitosan Hydrogel Can Prevent Bone Formation in Both Rat Skull and Fibula Bone Defects

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