Chitosan/sericin blend membranes for adsorption of bovine serum albumin

Abstract

Chitosan/sericin blend membranes for adsorption of bovine serum albumin (BSA) were investigated. A preliminary screening test was carried out to identify a suitable chitosan/sericin blend ratio. The blend membrane with a chitosan/sericin mass ratio of 4:1 was found to have a maximum adsorption capacity (qm) of 25.3 g/m2, which is 45 % greater than the maximum adsorption capacity of the pure chitosan membrane. The adsorption‐desorption cycle studies showed that the chitosan/sericin blend affinity membrane could be reused, and over 80 % of the original adsorption capacity was retained after regeneration. Compared to other affinity membranes for BSA adsorption, the chitosan/sericin membrane exhibited a significantly high adsorption capacity with a comparable adsorption rate.

Experimental methods in chemical engineering: Ultraviolet visible spectroscopy—UV‐Vis

“…Engineers also characterize polymer impregnation, measure the size distribution of emulsions, release rates control of organic compounds, and encapsulation and release rates of antibiotics . UV‐vis spectroscopy is a powerful tool to calculate the reaction rates .…”

Recent Developments of Liquid Chromatography Stationary Phases for Compound Separation: From Proteins to Small Organic Compounds

“…Aside from small organic molecules, polypeptides also have potential as affinity ligands for protein capture. Sericin, a silkworm polypeptide, upon blending with chitosan membranes with a ratio of 4:1, was capable of recognizing serum albumin and led to a 45% increase in adsorption capacity compared to chitosan alone [ 65 ]. For antibody purification, an alternative for affinity ligands is tryptamine, which can bind the nucleotide-binding site on the Fab domain of the antibodies [ 66 ].…”

Molecularly Imprinted Affinity Membrane: A Review

“…For example, sericin, a silkworm polypeptide, can increase the adsorption capacity of a chitosan/sericin membrane (4:1 w/w) toward serum albumin up to 45% higher than the sole chitosan membrane. 47 Tryptamine is an alternative ligand that can be used to purify antibodies because it can bind the nucleotide-binding site on the Fab (fragment antigen binding) domain of the antibodies. 48 In this way, the purification of monoclonal and polyclonal antibodies from the cell culture media of ascites fluids is possible.…”

“…The initial development of affinity membranes was a modification of common microporous membranes such as polyethylene (PE), polypropylene (PPr), polycarbonate (PC), polysulfone (PS), and other hydrocarbon polymers. 60 Biobased membranes, such as cellulose 23 and chitosan, 47 were also popular choices for affinity membrane applications by modifying the membrane surface to recognize specific target molecules ranging from metal ions to peptides and proteins, thus widening the potential application. Candidate polymers should have resistance toward solvent, and even a small amount of swelling could compromise selectivity toward the target molecule.…”

“…Polypeptides are also attractive options of affinity ligands for protein separation. For example, sericin, a silkworm polypeptide, can increase the adsorption capacity of a chitosan/sericin membrane (4:1 w/w) toward serum albumin up to 45% higher than the sole chitosan membrane . Tryptamine is an alternative ligand that can be used to purify antibodies because it can bind the nucleotide-binding site on the Fab (fragment antigen binding) domain of the antibodies .…”