Chitosan scaffolds for cartilage regeneration: influence of different ionic crosslinkers on biomaterial properties

Izzo, Daniela; Palazzo, Barbara; Scalera, Francesca; Gullotta, Fabiana; lapesa, Velia; Scialla, Stefania; Sannino, Alessandro; Gervaso, Francesca

doi:10.1080/00914037.2018.1525538

Cited by 25 publications

(13 citation statements)

References 35 publications

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“…B) showed lower swelling percentage compared to the untreated analogues. More specifically, Q was in the range between 40 and 80, for chitosan scaffolds with only DM, and in the range between 40 and 50, for l ‐Arg treated magnetic scaffolds. Moreover, l ‐Arg stabilized magnetic scaffolds showed no significant changes in the swelling behavior as a function of DMs percentages.…”

Section: Resultsmentioning

confidence: 98%

“…The water binding ability of a scaffold is an important feature for tissue regeneration. It could affect the scaffold microstructure and mechanical stability, facilitating the cellular infiltration into the 3D matrix . The water absorption (or swelling ratio, Q) of Cs and Cs/DM scaffolds treated with l ‐Arg and untreated was measured at a physiological pH, in PBS and the results are shown in Figure A,B, respectively.…”

Section: Resultsmentioning

confidence: 99%

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Bioactive chitosan‐based scaffolds with improved properties induced by dextran‐grafted nano‐maghemite and l‐arginine amino acid

Scialla

Barca

Palazzo

et al. 2019

J Biomedical Materials Res

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Over the past years, fundamentals of magnetism opened a wide research area of interest, in the field of tissue engineering and regenerative medicine. The integration of magnetic nanoarchitectures into synthetic/natural scaffold formulations allowed obtaining “on demand” responsive structures able to guide the regeneration process. The aim of this work was the design and characterization of three‐dimensional (3D) chitosan‐based scaffolds containing dextran‐grafted maghemite nanoarchitectures (DM) and functionalized with l‐arginine (l‐Arg) amino acid as bioactive agent. A homogeneous pore distribution and a high degree of interconnection were obtained for all the structures with DMs, which resulted well distributed inside the polymer matrix. All the results suggest that the simultaneous presence of DMs and l‐Arg conferred interesting mechano‐structural and bioactive properties toward osteoblast‐like and human mesenchymal stem cells, differentially stimulating their proliferation both in the absence and in the presence of a time‐dependent magnetic field. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1244–1252, 2019.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Bioactive chitosan‐based scaffolds with improved properties induced by dextran‐grafted nano‐maghemite and l‐arginine amino acid

Scialla

Barca

Palazzo

et al. 2019

J Biomedical Materials Res

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“…However, CH is a soft biomaterial characterized by low mechanical resistance, especially under hydrated conditions, which represents one of the main limitations in using it without the addition of other components. CH efficiently complexes metal ions or nanoparticles, natural or synthetic anionic species (such as lipids, proteins, DNA), and polyelectrolytes (such as tripolyphosphate), or is blended with other polymers or functionalized with bioactive agents to enhance its mechanical properties. This was also achieved by integrating bioceramics, in particular HA, into the CH matrix for scaffolding fabrication, showing that CH/HA scaffolds were characterized by a significant enhancement of mechanical strength with an increased osteoconductivity .…”

Section: Soft Matrix‐based Biocompositesmentioning

confidence: 99%

Additive Manufacturing Approaches for Hydroxyapatite‐Reinforced Composites

Milazzo

Negrini

Scialla

et al. 2019

Adv Funct Materials

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116

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Additive manufacturing (AM) techniques have gained interest in the tissue engineering field, thanks to their versatility and unique possibilities of producing constructs with complex macroscopic geometries and defined patterns. Recently, composite materials-namely, heterogeneous biomaterials identified as continuous phase (matrix) and reinforcement (filler)-have been proposed as inks that can be processed by AM to obtain scaffolds with improved biomimetic and bioactive properties. Significant efforts have been dedicated to hydroxyapatite (HA)-reinforced composites, especially targeting bone tissue engineering, thanks to the chemical similarities of HA with respect to mineral components of native mineralized tissues. Herein, applications of AM techniques to process HA-reinforced composites and biocomposites for the production of scaffolds with biological matrices, including cellular tissues, are reviewed. The primary outcomes of recent investigations in terms of morphological, structural, and in vitro and in vivo biological properties of the materials are discussed. The approaches based on the nature of the matrices employed to embed the HA reinforcements and produce the tissue substitutes are classified, and a critical discussion is provided on the presented state of the art as well as the future perspectives, to offer a comprehensive picture of the strategies investigated as well as challenges in this emerging field of materiomics.

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“…L-Arg is a conditionally essential amino acid, involved as a precursor in many important biochemical pathways in cellular physiology (i.e., nitric oxide pathway involved in nerve regeneration) [33][34][35], and therefore an enhancer of some key cell processes, e.g., collagen synthesis, T-cell mediated responses, and also the release of pituitary hormones [36]. For these reasons, in our previous studies [37][38][39], we used it as a non-toxic ionic cross-linking biomolecule for chitosan structures, hopefully eliciting enhanced biological responses. The results showed that ARG was able to improve the in vitro stability of chitosan 3D structures, such as scaffolds and electrospun membranes.…”

Section: Introductionmentioning

confidence: 99%

Development of Injectable Thermosensitive Chitosan-Based Hydrogels for Cell Encapsulation

et al. 2020

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The three-dimensional complexity of the native extracellular matrix (ECM) suggests switching from 2D to 3D culture systems for providing the cells with an architecture more similar to the physiological environment. Reproducing the three-dimensionality in vitro can guarantee beneficial effects in terms of cell growth, adhesion, proliferation, and/or their differentiation. Hydrogels have the same tailorable physico-chemical and biological characteristics as ECM materials. In this study, we propose a thermoresponsive chitosan-based hydrogel that gels thanks to the addition of organic and inorganic salt solutions (beta-glycerolphosphate and sodium hydrogen carbonate) and is suitable for cell encapsulation allowing obtaining 3D culture systems. Physico-chemical analyses showed that the hydrogel formulations jellify at physiological conditions (37 °C, pH 7.4), are stable in vitro up to three weeks, have high swelling ratios and mechanical stiffness suitable for cellular encapsulation. Moreover, preliminary biological tests underlined the pronounced biocompatibility of the system. Therefore, these chitosan-based hydrogels are proposed as valid biomaterials for cell encapsulation.

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Chitosan scaffolds for cartilage regeneration: influence of different ionic crosslinkers on biomaterial properties

Cited by 25 publications

References 35 publications

Bioactive chitosan‐based scaffolds with improved properties induced by dextran‐grafted nano‐maghemite and l‐arginine amino acid

Bioactive chitosan‐based scaffolds with improved properties induced by dextran‐grafted nano‐maghemite and l‐arginine amino acid

Additive Manufacturing Approaches for Hydroxyapatite‐Reinforced Composites

Development of Injectable Thermosensitive Chitosan-Based Hydrogels for Cell Encapsulation

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