Chitosan production from hemicellulose hydrolysate of corn straw: impact of degradation products on Rhizopus oryzae growth and chitosan fermentation

Tai, Chao; Li, S.; Xu, Qing; Ying, Hanjie; Huang, He; Ouyang, Pingkai

doi:10.1111/j.1472-765x.2010.02893.x

Cited by 35 publications

(16 citation statements)

References 28 publications

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“…Encouraged by the aforementioned cross tolerance toward HMF and vanillin, we further exploit the growth of the furfural tolerant cells in LB supplemented with real hemicellulose hydrolysate and cellulosic hydrolysates. Hemicellulose hydrolysate used in this work was prepared using the method of dilute sulfuric acid hydrolysis with sugarcane bagasse as the feedstock (Betancur and Pereira, 2010; Cheng et al, 2007; Tai et al, 2010). A hydrolysate with 4.62 g/L glucose, 16.79 g/L xylose, 1.22 g/L furfural, 0.05 g/L HMF, 0.13 g/L formic acid, and 3.93 g/L acetic acid was obtained, which is comparable with those reported in the literature (Cheng et al, 2007).…”

Section: Resultsmentioning

confidence: 99%

Clinical practice patterns and cost effectiveness of human epidermal growth receptor 2 testing strategies in breast cancer patients

Phillips

Marshall

Haas

et al. 2009

Cancer

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Lignocellulosic biomass is regarded as the most viable source of feedstock for industrial biorefinery, but the harmful inhibitors generated from the indispensable pretreatments prior to fermentation remain a daunting technical hurdle. Using an exogenous regulator, irrE, from the radiation‐resistant Deinococcus radiodurans, we previously showed that a novel global regulator engineering (GRE) approach significantly enhanced tolerances of Escherichia coli to alcohol and acetate stresses. In this work, an irrE library was subjected to selection under various stresses of furfural, a typical hydrolysate inhibitor. Three furfural tolerant irrE mutants including F1‐37 and F2‐1 were successfully obtained. The cells containing these mutants reached OD600 levels of 4‐ to 16‐fold of that for the pMD18T cells in growth assay under 0.2% (v/v) furfural stress. The cells containing irrE F1‐37 and F2‐1 also showed considerably reduced intracellular oxygen species (ROS) levels under furfural stress. Moreover, these two irrE mutants were subsequently found to confer significant cross tolerances to two other most common inhibitors, 5‐hydroxymethyl‐2‐furaldehyde (HMF), vanillin, as well as real lignocellulosic hydrolysates. When evaluated in Luria–Bertani (LB) medium supplemented with corn stover cellulosic hydrolysate (prepared with a solid loading of 30%), the cells containing the mutants exhibited lag phases markedly shortened by 24–44 h in comparison with the control cells. This work thus presents a promising step forward to resolve the inhibitor problem for E. coli. From the view of synthetic biology, irrE can be considered as an evolvable “part” for various stresses. Furthermore, this GRE approach can be extended to exploit other exogenous global regulators from extremophiles, and the native counterparts in E. coli, for eliciting industrially useful phenotypes. Biotechnol. Bioeng. 2012; 109: 3133–3142. © 2012 Wiley Periodicals, Inc.

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Section: Resultsmentioning

confidence: 99%

Clinical practice patterns and cost effectiveness of human epidermal growth receptor 2 testing strategies in breast cancer patients

Phillips

Marshall

Haas

et al. 2009

Cancer

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“…This result displays that the biomass yield was higher than that of in mixed sugars medium (6.02 g/L). Tai et al had reported that hemicellulose hydrolysate increased R. oryzae AS 3.819 growth by 10.2% compared to the growth in xylose medium [3].…”

Section: Effects Of Glucose and Xylose On Fungal Chitosan Productionmentioning

confidence: 99%

“…The structure of chitosan is a linear of polysaccharide consisted of abundant D-glucosamine and little N-acetyl-D-glucosamine residues connected by b-type glycosidic bonds [2]. Chitosan has unique characteristics such as biodegradability, biocompatibility and inhibitory action [3], so it could be applied in extensive fields, such as pharmaceutics and cosmetology [4][5][6]. Chitosan is traditionally produced from the waste of shellfish exoskeleton.…”

Section: Introductionmentioning

confidence: 99%

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Fungal chitosan production using xylose rich of corn stover prehydrolysate by Rhizopus oryzae

Yang

Lai

et al. 2017

Biotechnology & Biotechnological Equipment

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Xylose rich of lignocellulosic prehydrolysate could be used for fungal chitosan production effectively by Rhizopus oryzae. When 15 g/L xylose was used as carbon source for R. oryzae AS 3.819 fermentation, almost all of the xylose was consumed for cell growth (4.33 g/L of biomass) and the chitosan extraction yield was 0.11 g/g biomass. Corn stover prehydrolysate by dilute acid-assisted steam explosion pretreatment that contained 30 g/L xylose and 10 g/L glucose was fermented by R. oryzae AS 3.819 for 72 h, the biomass and the chitosan extraction yield were 10.96 g/L and 0.09 g/g biomass. The degree of deacetylation of the fungal chitosan derived from corn stover prehydrolysate (91.27%) was higher than that of the commercial chitosan extracted from natural shellfish exoskeleton (87.25%), and the viscosity of the fungal chitosan derived from corn stover prehydrolysate (2.67 mPaÁs) had a large decrease compared to the commercial chitosan (22.25 mPaÁs). The functional groups and thermostability of the fungal chitosan were proofed by Infrared (IR) spectra and Derivative thermogravimetric (DTG), respectively.

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“…Overall, mycelial morphology has a strong effect on the physical properties of the broth and often leads to a number of different problems in large bioreactors with respect to gas dispersion, as well as mass and heat transfer (Higashiyama et al 2002). There are many reports that discuss the size and shape of fungal mycelial pellets (Xu et al 2010; Tai et al 2010), but little is known about the true features of the internal pellet structure, including geometry and mycelial viability (Hamanaka et al 2001). Interestingly, pellets with a moderate compactness are the more productive morphological form for the production of ARA-rich oil, compared to free filamentous mycelia.…”

Section: Introductionmentioning

confidence: 99%

An efficient multi-stage fermentation strategy for the production of microbial oil rich in arachidonic acid in Mortierella alpina

Zhang

Peng³

et al. 2017

Bioresour. Bioprocess.

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BackgroundFungal morphology and aeration play a significant role in the growth process of Mortierella alpina. The production of microbial oil rich in arachidonic acid (ARA) in M. alpina was enhanced by using a multi-stage fermentation strategy which combined fed-batch culture with precise control of aeration and agitation rates at proper times.ResultsThe fermentation period was divided into four stages according to the cultivation characteristics of M. alpina. The dissolved oxygen concentration was well suited for ARA biosynthesis. Moreover, the ultimate dry cell weight (DCW), lipid, and ARA yields obtained using this strategy reached 41.4, 22.2, 13.5 g/L, respectively. The respective values represent 14.8, 25.8, and 7.8% improvements over traditional fed-batch fermentation processes.ConclusionsThis strategy provides promising control insights for the mass production of ARA-rich oil on an industrial scale. Pellet-like fungal morphology was transformed into rice-shaped particles which were beneficial for oxygen transfer and thus highly suitable for biomass accumulation.

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Chitosan production from hemicellulose hydrolysate of corn straw: impact of degradation products on Rhizopus oryzae growth and chitosan fermentation

Abstract: This work for the first time reported chitosan production from HH. Chitosan production can be greatly enhanced by cheap chemicals such as inhibitors in HH.

Cited by 35 publications

References 28 publications

Clinical practice patterns and cost effectiveness of human epidermal growth receptor 2 testing strategies in breast cancer patients

Clinical practice patterns and cost effectiveness of human epidermal growth receptor 2 testing strategies in breast cancer patients

Fungal chitosan production using xylose rich of corn stover prehydrolysate by Rhizopus oryzae

An efficient multi-stage fermentation strategy for the production of microbial oil rich in arachidonic acid in Mortierella alpina

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