Chitosan, Polyethylene Glycol and Polyvinyl Alcohol Modified MgFe2O4 Ferrite Magnetic Nanoparticles in Doxorubicin Delivery: A Comparative Study In Vitro

Ramnandan, Deevak; Mokhosi, Seipati; Daniels, Aliscia; Singh, Moganavelli

doi:10.3390/molecules26133893

Cited by 39 publications

(25 citation statements)

References 89 publications

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“…In addition to providing stability in an aqueous medium, CS was included for its many cationic amine groups that can electrostatically bind to the negatively charged phosphate groups of the pDNA and hydroxyl groups, which are useful binding sites for many other molecules [33]. The medium molecular weight CS was chosen based on previous studies by the authors, which showed that this molecular weight and the chosen concentration was easy to conjugate to the AuNPs, in addition to producing stable NPs of favorable sizes [33,64,65]. The use of His was based on the need for the early endosomal escape of the gene cargo before it can be degraded.…”

Section: Discussionmentioning

confidence: 99%

Histidine-Tagged Folate-Targeted Gold Nanoparticles for Enhanced Transgene Expression in Breast Cancer Cells In Vitro

et al. 2021

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Nanotechnology has emerged as a promising treatment strategy in gene therapy, especially against diseases such as cancer. Gold nanoparticles (AuNPs) are regarded as favorable gene delivery vehicles due to their low toxicity, ease of synthesis and ability to be functionalized. This study aimed to prepare functionalized AuNPs (FAuNPs) and evaluate their folate-targeted and nontargeted pCMV-Luc-DNA delivery in breast cancer cells in vitro. CS was added to induce stability and positive charges to the AuNPs (Au-CS), histidine (Au-CS-His) to enhance endosomal escape and folic acid for folate-receptor targeting (Au-CS-FA-His). The FAuNP:pDNA nanocomplexes possessed favorable sizes (<135 nm) and zeta potentials (<−20 mV), strong compaction efficiency and were capable of pDNA protection against nuclease degradation. These nanocomplexes showed minimal cytotoxicity (>73% cell viability) and enhanced transgene activity. The influence of His was notable in the HER2 overexpressing SKBR3 cells, which produced higher gene expression. Furthermore, the FA-targeted nanocomplexes enhanced receptor-mediated endocytosis, especially in MCF-7 cells, as confirmed by the receptor competition assay. While the role of His may need further optimization, the results achieved suggest that these FAuNPs may be suitable gene delivery vehicles for breast cancer therapeutics.

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Section: Discussionmentioning

confidence: 99%

Histidine-Tagged Folate-Targeted Gold Nanoparticles for Enhanced Transgene Expression in Breast Cancer Cells In Vitro

et al. 2021

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“…This porous nature of MSNs allows for the possible combination delivery of therapeutic genes and drugs, which can improve biological activity [75]. Quercetin-encapsulated silica NPs have demonstrated potential against Cu-induced oxidative stress observed in neurodegenerative diseases [76] Iron oxides, commonly referred to as magnetic NPs (MNPs), including maghemites, magnetites and ferrites, have been widely studied in nanomedicine due to their low cytotoxicity, biodegradability, stability, magnetization, biocompatibility, low sensitivity to oxidation and reactive surfaces, non-carcinogenicity, and ease of synthesis and modification [77]. The target-specific delivery of MNPs can be achieved by the process of magnetofection that uses an external magnetic field to guide their delivery.…”

Section: Nanoparticles and Nanomedicinementioning

confidence: 99%

Nanomedicine for Neurodegenerative Disorders: Focus on Alzheimer’s and Parkinson’s Diseases

Jagaran

Singh

2021

IJMS

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Neurodegenerative disorders involve the slow and gradual degeneration of axons and neurons in the central nervous system (CNS), resulting in abnormalities in cellular function and eventual cellular demise. Patients with these disorders succumb to the high medical costs and the disruption of their normal lives. Current therapeutics employed for treating these diseases are deemed palliative. Hence, a treatment strategy that targets the disease’s cause, not just the symptoms exhibited, is desired. The synergistic use of nanomedicine and gene therapy to effectively target the causative mutated gene/s in the CNS disease progression could provide the much-needed impetus in this battle against these diseases. This review focuses on Parkinson’s and Alzheimer’s diseases, the gene/s and proteins responsible for the damage and death of neurons, and the importance of nanomedicine as a potential treatment strategy. Multiple genes were identified in this regard, each presenting with various mutations. Hence, genome-wide sequencing is essential for specific treatment in patients. While a cure is yet to be achieved, genomic studies form the basis for creating a highly efficacious nanotherapeutic that can eradicate these dreaded diseases. Thus, nanomedicine can lead the way in helping millions of people worldwide to eventually lead a better life.

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“…Dox has often been used in MNP studies, in bound form. 33 , 34 TurboBeads™ and NiNRs both exhibit ferromagnetic ordering, retaining strong remnant magnetism even in the absence of an applied field from the coil array. The other MNPs exhibit paramagnetic behaviour, losing nearly all their remnant magnetism in the absence of an externally applied field.…”

Section: Discussionmentioning

confidence: 99%

Parallel Multichannel Assessment of Rotationally Manipulated Magnetic Nanoparticles

Hussain

Mair

Willis

et al. 2022

NSA

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Background Rotational manipulation of chains or clusters of magnetic nanoparticles (MNPs) offers a means for directed translation and payload delivery that should be explored for clinical use. Multiple MNP types are available, yet few studies have performed side-by-side comparisons to evaluate characteristics such as velocity, movement at a distance, and capacity for drug conveyance or dispersion. Purpose Our goal was to design, build, and study an electric device allowing simultaneous, multichannel testing (e.g., racing) of MNPs in response to a rotating magnetic field. We would then select the “best” MNP and use it with optimized device settings, to transport an unbound therapeutic agent. Methods A magnetomotive system was constructed, with a Helmholtz pair of coils on either side of a single perpendicular coil, on top of which was placed an acrylic tray having multiple parallel lanes. Five different MNPs were tested: graphene-coated cobalt MNPs (TurboBeads™), nickel nanorods, gold-iron alloy MNPs, gold-coated Fe 3 O 4 MNPs, and uncoated Fe 3 O 4 MNPs. Velocities were determined in response to varying magnetic field frequencies (5–200 Hz) and heights (0–18 cm). Velocities were normalized to account for minor lane differences. Doxorubicin was chosen as the therapeutic agent, assayed using a CLARIOstar Plus microplate reader. Results The MMS generated a maximal MNP velocity of 0.9 cm/s. All MNPs encountered a “critical” frequency at 20–30 Hz. Nickel nanorods had the optimal response based on tray height and were then shown to enable unbound doxorubicin dispersion along 10.5 cm in <30 sec. Conclusion A rotating magnetic field can be conveniently generated using a three-coil electromagnetic device, and used to induce rotational and translational movement of MNP aggregates over mesoscale distances. The responses of various MNPs can be compared side-by-side using multichannel acrylic trays to assess suitability for drug delivery, highlighting their potential for further in vivo applications.

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Chitosan, Polyethylene Glycol and Polyvinyl Alcohol Modified MgFe2O4 Ferrite Magnetic Nanoparticles in Doxorubicin Delivery: A Comparative Study In Vitro

Cited by 39 publications

References 89 publications

Histidine-Tagged Folate-Targeted Gold Nanoparticles for Enhanced Transgene Expression in Breast Cancer Cells In Vitro

Histidine-Tagged Folate-Targeted Gold Nanoparticles for Enhanced Transgene Expression in Breast Cancer Cells In Vitro

Nanomedicine for Neurodegenerative Disorders: Focus on Alzheimer’s and Parkinson’s Diseases

Parallel Multichannel Assessment of Rotationally Manipulated Magnetic Nanoparticles

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