Chitosan(PEO)/silica hybrid nanofibers as a potential biomaterial for bone regeneration

Toskas, Georgios; Cherif, Chokri; Hund, Rolf-Dieter; Laourine, Ezzeddine; Mahltig, Boris; Fahmi, Amir; Heinemann, Christiane; Hanke, Thomas

doi:10.1016/j.carbpol.2013.01.068

Cited by 130 publications

(67 citation statements)

References 40 publications

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“…40 Mineralization of hMSCs at the middle of the culture period is the evidence for its capability to secrete inorganic phosphates. This can be examined by observing the mineral deposition on the cell surface through microscopy or other quantitative assays.…”

Section: Mineralization Of Hmsc By Sem/edsmentioning

confidence: 99%

Response of human mesenchymal stem cells to intrafibrillar nanohydroxyapatite content and extrafibrillar nanohydroxyapatite in biomimetic chitosan/silk fibroin/nanohydroxyapatite nanofibrous membrane scaffolds

Lai

Shalumon

Chen

2015

IJN

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Incorporation of nanohydroxyapatite (nHAP) within a chitosan (CS)/silk fibroin (SF) nanofibrous membrane scaffold (NMS) may provide a favorable microenvironment that more closely mimics the natural bone tissue physiology and facilitates enhanced osteogensis of the implanted cell population. In this study, we prepared pristine CS/SF NMS, composite CS/SF/nHAP NMS containing intrafibrillar nHAP by in situ blending of 10% or 30% nHAP before the electrospinning step, and composite CS/SF/nHAP NMS containing extrafibrillar nHAP by depositing 30% nHAP through alternative soaking surface mineralization. We investigated the effect of the incorporation of HAP nanoparticles on the physicochemical properties of pristine and composite NMS. We confirmed the presence of ~30 nm nHAP in the composite nanofibrous membranes by thermogravimetry analysis (TGA), X-ray diffraction (XRD), and scanning electron microscopy (SEM), either embedded in or exposed on the nanofiber. Nonetheless, the alternative soaking surface mineralization method drastically influenced the mechanical properties of the NMS with 88% and 94% drop in Young’s modulus and ultimate maximum stress. Using in vitro cell culture experiments, we investigated the effects of nHAP content and location on proliferation and osteogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs). The proliferation of hMSCs showed no significant difference among pristine and composite NMS. However, the extent of osteogenic differentiation of hMSCs was found to be positively correlated with the content of nHAP in the NMS, while its location within the nanofiber played a less significant role. In vivo experiments were carried out with hMSCs seeded in CS/SF/30%nHAP NMS prepared by in situ blending and subcutaneous implantation in nude mice. Micro-computed tomography images as well as histological and immunohistochemical analysis of the retrieved hMSCs/NMS construct 1 and 2 months postimplantation indicated that NMS had the potential for bone regeneration and can be suggested as a promising scaffold for bone tissue engineering.

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Section: Mineralization Of Hmsc By Sem/edsmentioning

confidence: 99%

Response of human mesenchymal stem cells to intrafibrillar nanohydroxyapatite content and extrafibrillar nanohydroxyapatite in biomimetic chitosan/silk fibroin/nanohydroxyapatite nanofibrous membrane scaffolds

Lai

Shalumon

Chen

2015

IJN

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“…Chitosan, an ideal biomaterial with excellent biocompatibility and antibacterial capability, has been widely applied in tissue engineering and bone repair. [15][16][17][18][19] Therefore, we chose chitosan as the organic phase to fabricate the inorganic/organic composite porous scaffold for bone regeneration. First, we prepared HAP and WH hollow microspheres by using creatine phosphate (CP) disodium salt as an organic phosphorus source in aqueous solution by microwave-assisted hydrothermal method.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects

Zhou

Chen

et al. 2017

IJN

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Hydroxyapatite (HAP; Ca 10 (PO 4 ) 6 (OH) 2 ) and whitlockite (WH; Ca 18 Mg 2 (HPO 4 ) 2 (PO 4 ) 12 ) are widely utilized in bone repair because they are the main components of hard tissues such as bones and teeth. In this paper, we synthesized HAP and WH hollow microspheres by using creatine phosphate disodium salt as an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method. Then, we prepared HAP/chitosan and WH/chitosan composite membranes to evaluate their biocompatibility in vitro and prepared porous HAP/chitosan and WH/chitosan scaffolds by freeze drying to compare their effects on bone regeneration in calvarial defects in a rat model. The experimental results indicated that the WH/chitosan composite membrane had a better biocompatibility, enhancing proliferation and osteogenic differentiation ability of human mesenchymal stem cells than HAP/chitosan. Moreover, the porous WH/chitosan scaffold can significantly promote bone regeneration in calvarial defects, and thus it is more promising for applications in tissue engineering such as calvarial repair compared to porous HAP/chitosan scaffold.

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“…The fibre diameters for both porous class I and II hybrids are larger in comparison with those produced by conventional SBS in the work presented here, and electrospun hybrid fibres were produced by others. As previously mentioned, authors achieved fibres diameters of 192.6 ± 8 and 239 ± 13 nm by electrospinning gelatin/silica and chitosan-PEO/silica hybrids, respectively [47,49]. Despite the relatively large fibre size distribution, class II fibres in the range of 700 nm were obtained.…”

Section: Porous Sphere and Fibre Morphologies Fibre Size Distributionsmentioning

confidence: 81%

“…As evaporation continues viscosity is raised, and past a critical point the polycondensing sol-gel will no longer spin into fibres. The electrospinning technique has been extended to inorganic-organic hybrids formulations, commonly using tetraethyl orthosilicate (TEOS) as the inorganic phase with various natural and synthetic organic phases, including PLA [46], chitosan/poly(ethylene oxide) (PEO) [47], and gelatin functionalised with (3-glycidyloxypropyl)trimethoxysilane (GPTMS) [48,49]. GPTMS can be used to provide the inorganic network [48], as well as act as a crosslinker between inorganic and organic phases [49].…”

Section: Introductionmentioning

confidence: 99%

Hybrid sol–gel inorganic/gelatin porous fibres via solution blow spinning

et al. 2017

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Hybrid sol-gel inorganic-organic fibres offer great potential in tissue engineering and regenerative medicine. A significant challenge is to process them using scalable technologies into useful scaffolds that provide control over fibre diameter, morphology, mechanical properties, ion release, degradation and cell response. In this work we develop formulations that are amenable to processing via solution blow spinning (SBS), a rapid technique using simple equipment to spray nano-/ micro-fibres without any electric fields. The technique is extended to produce porous class I and II hybrid fibres using cryogenic SBS, with formulations developed based on tetraethyl orthosilicate/gelatin that are relatively facile to lyophilise. The formulations developed here take advantage of the reversible thermally activated conformation change of gelatin in aqueous solutions from random coil to triple helix to enable viscosity tuning and therefore fibre spinning. Gelatin is functionalised with (3-glycidyloxypropyl)trimethoxysilane to produce class II hybrids which exhibit controllable time-and temperature-dependent viscosity profiles which can be tuned for spinning into highly porous fibres.Ryan D. Greenhalgh and William S. Ambler are joint first authors due to equal contribution.

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Chitosan(PEO)/silica hybrid nanofibers as a potential biomaterial for bone regeneration

Cited by 130 publications

References 40 publications

Response of human mesenchymal stem cells to intrafibrillar nanohydroxyapatite content and extrafibrillar nanohydroxyapatite in biomimetic chitosan/silk fibroin/nanohydroxyapatite nanofibrous membrane scaffolds

Response of human mesenchymal stem cells to intrafibrillar nanohydroxyapatite content and extrafibrillar nanohydroxyapatite in biomimetic chitosan/silk fibroin/nanohydroxyapatite nanofibrous membrane scaffolds

Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects

Hybrid sol–gel inorganic/gelatin porous fibres via solution blow spinning

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