Chitosan oligosaccharide-mediated attenuation of LPS-induced inflammation in IPEC-J2 cells is related to the TLR4/NF-κB signaling pathway

Shi, Lin; Fang, Biao; Yong, Yanhong; Li, Xuewen; Gong, Dongliang; Li, Junyu; Yu, Tianyue; Gooneratne, Ravi; Gao, Zhenhua; Li, Sidong

doi:10.1016/j.carbpol.2019.05.036

Cited by 75 publications

(46 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The antimicrobial properties of chitosan and its degradation products depend on many factors, including their source and concentration, molecular weight and deacetylation degree, and the strain of the microorganism on which they were tested (Kyoon et al, 2003; Liu et al, 2006; Li et al, 2014; Laokuldilok et al, 2017; Bonilla et al, 2019; Shi et al, 2019). It was found that in the case of COS, their DP with a value of not less than five is essential for antibacterial activity of fully deacetylated COS (Li et al, 2014).…”

Section: Biological Activity Of Chitin and Its Derivativesmentioning

confidence: 99%

Enzymatic Modifications of Chitin, Chitosan, and Chitooligosaccharides

Kaczmarek

Struszczyk‐Świta

et al. 2019

Front. Bioeng. Biotechnol.

247

157

View full text Add to dashboard Cite

Chitin and its N-deacetylated derivative chitosan are two biological polymers that have found numerous applications in recent years, but their further deployment suffers from limitations in obtaining a defined structure of the polymers using traditional conversion methods. The disadvantages of the currently used industrial methods of chitosan manufacturing and the increasing demand for a broad range of novel chitosan oligosaccharides (COS) with a fully defined architecture increase interest in chitin and chitosan-modifying enzymes. Enzymes such as chitinases, chitosanases, chitin deacetylases, and recently discovered lytic polysaccharide monooxygenases had attracted considerable interest in recent years. These proteins are already useful tools toward the biotechnological transformation of chitin into chitosan and chitooligosaccharides, especially when a controlled non-degradative and well-defined process is required. This review describes traditional and novel enzymatic methods of modification of chitin and its derivatives. Recent advances in chitin processing, discovery of increasing number of new, well-characterized enzymes and development of genetic engineering methods result in rapid expansion of the field. Enzymatic modification of chitin and chitosan may soon become competitive to conventional conversion methods.

show abstract

Section: Biological Activity Of Chitin and Its Derivativesmentioning

confidence: 99%

Enzymatic Modifications of Chitin, Chitosan, and Chitooligosaccharides

Kaczmarek

Struszczyk‐Świta

et al. 2019

Front. Bioeng. Biotechnol.

247

157

View full text Add to dashboard Cite

show abstract

“…Endotoxins, such as LPS,are the key components of the cell wall of gram-negative bacteria [24]. LPS, from Escherichia coli O111:B4, is commonly used as an inducer to immune cells [4,30], which binds to the toll-like receptors (TLRs) and initiates in ammation [34].…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory effects of DhHP-6 in LPS-induced RAW264.7 macrophages and carrageenan-induced air pouch model in rat

Meng¹,

Ke²,

Xu³

et al. 2021

Preprint

View full text Add to dashboard Cite

DhHP-6 (Deuterohemin-Ala-His-Thr-Val-Glu-Lys) is a novel peptide mimic of peroxidases that was previously designed in our laboratory. Here, we explored the anti-inflammatory potential of DhHP-6 against lipopolysaccharide(LPS)stimulated inflammatory response in RAW264.7 cells and carrageenan-induced air pouch model rats. DhHP-6 treatment dramatically attenuated the production of nitric oxide (NO), IL-6, andTNF-α in LPS induced RAW264.7 cells. Also, it blocked phosphorylation and degradation of IκBα and suppressed the nuclear translocation of p65. DhHP-6 (0.2, 0.6, and 2.0 mg/kg) significantly reduced the levels of total proteins and WBC counts in the exudates of the air pouch model rats. Moreover, MDA contents in the plasma of rats were reduced and SOD activities were enhanced in the DhHP-6-treatment group. Our results strongly show the effectiveness of DhHP-6 as an anti-inflammatory agent. The mechanism could be related to the reduction of Reactive oxygen species(ROS), inhibition of NF-κB nuclear translocation, and reduction of pro-inflammatory cytokines.

show abstract

“…The histological analysis showed early reepithelialization and angiogenesis. COS has previously demonstrated anti-inflammatory activity by hampering the responses from lipopolysaccharides and inhibiting mitogen-activated protein kinase-dependent pathways [128,129]. Oligosaccharide synthesis has historically been a laborintensive, inefficient, and time-consuming synthesis method.…”

Section: Oligosaccharide-derived Polymersmentioning

confidence: 99%

Supramolecular Biopolymers for Tissue Engineering

Pérez-Pedroza

Ávila-Ramírez

Khan

et al. 2021

Advances in Polymer Technology

View full text Add to dashboard Cite

Supramolecular biopolymers (SBPs) are those polymeric units derived from macromolecules that can assemble with each other by noncovalent interactions. Macromolecular structures are commonly found in living systems such as proteins, DNA/RNA, and polysaccharides. Bioorganic chemistry allows the generation of sequence-specific supramolecular units like SBPs that can be tailored for novel applications in tissue engineering (TE). SBPs hold advantages over other conventional polymers previously used for TE; these materials can be easily functionalized; they are self-healing, biodegradable, stimuli-responsive, and nonimmunogenic. These characteristics are vital for the further development of current trends in TE, such as the use of pluripotent cells for organoid generation, cell-free scaffolds for tissue regeneration, patient-derived organ models, and controlled delivery systems of small molecules. In this review, we will analyse the 3 subtypes of SBPs: peptide-, nucleic acid-, and oligosaccharide-derived. Then, we will discuss the role that SBPs will be playing in TE as dynamic scaffolds, therapeutic scaffolds, and bioinks. Finally, we will describe possible outlooks of SBPs for TE.

show abstract

Chitosan oligosaccharide-mediated attenuation of LPS-induced inflammation in IPEC-J2 cells is related to the TLR4/NF-κB signaling pathway

Cited by 75 publications

References 51 publications

Enzymatic Modifications of Chitin, Chitosan, and Chitooligosaccharides

Enzymatic Modifications of Chitin, Chitosan, and Chitooligosaccharides

Anti-inflammatory effects of DhHP-6 in LPS-induced RAW264.7 macrophages and carrageenan-induced air pouch model in rat

Supramolecular Biopolymers for Tissue Engineering

Contact Info

Product

Resources

About