Chitosan Nanoparticles for the Linear Release of Model Cationic Peptide

Piras, Anna Maria; Sandreschi, Stefania; Maisetta, Giuseppantonio; Esin, Semih; Batoni, Giovanna; Chiellini, Emo

doi:10.1007/s11095-014-1615-9

Cited by 35 publications

(41 citation statements)

References 31 publications

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“…Surface zeta potentials of etched titanium (Ti-ox) and QAH grafted titanium (Ti-QAH) samples were determined by electroosmotic flow mapping (EOFM) technique, by using a Zetasizer Nano ZS, equipped with the surface accessory, and the surface potential dip cell (ZEN1020). The specimens were cut into squares of side 3.5 mm, attached to the peek holder by using cyanoacrylate glue, and cleaned by sonication in deionized water for 5 min at 25 • C. The was measured according to the manifacturer instruction (Malvern, UK) by using Ch nanoparticles as tracer, prepared according to Piras et al 2015 [56], re-dispersed in water and displaying the following features: diameter 252 ± 7.7 nm (PI 0.324); 23.3 ± 0.7 mV at pH3.8 (SI file for tracer preparation details). All the experiments were run at 25 • C, using tracer particle concentration of 0.05% wt.…”

Section: Surface Zeta Potentialmentioning

confidence: 99%

Antibacterial, Antibiofilm, and Antiadhesive Properties of Different Quaternized Chitosan Derivatives

Piras

Esin

Benedetti

et al. 2019

IJMS

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In the era of antimicrobial resistance, the identification of new antimicrobials is a research priority at the global level. In this regard, the attention towards functional antimicrobial polymers, with biomedical/pharmaceutical grade, and exerting anti-infective properties has recently grown. The aim of this study was to evaluate the antibacterial, antibiofilm, and antiadhesive properties of a number of quaternized chitosan derivatives that have displayed significant muco-adhesive properties and wound healing promotion features in previous studies. Low (QAL) and high (QAH) molecular weight quaternized chitosan derivatives were synthetized and further modified with thiol moieties or pendant cyclodextrin, and their antibacterial activity evaluated as minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC). The ability of the derivatives to prevent biofilm formation was assessed by crystal violet staining. Both QAL and QAH derivatives exerted a bactericidal and/or inhibitory activity on the growth of P. aeruginosa and S. epidermidis. The same compounds also showed marked dose-dependent anti-biofilm activity. Furthermore, the high molecular weight derivative (QAH) was used to functionalize titanium plates. The successful functionalization, demonstrated by electron microscopy, was able to partially inhibit the adhesion of S. epidermidis at 6 h of incubation. The shown ability of the chitosan derivatives tested to both inhibit bacterial growth and/or biofilm formation of clinically relevant bacterial species reveals their potential as multifunctional molecules against bacterial infections.

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Section: Surface Zeta Potentialmentioning

confidence: 99%

Antibacterial, Antibiofilm, and Antiadhesive Properties of Different Quaternized Chitosan Derivatives

Piras

Esin

Benedetti

et al. 2019

IJMS

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“…In deeper wounds, therapeutics, including AMPs, may be hampered by poor penetration to deeper layers, hindrance by wound debris, and/or breakdown by proteolytic enzymes. These challenges can be overcome through the use of tailored drug delivery systems such as nanoparticles for encapsulation and controlled release (39)(40)(41). Our recent report that SAAP-145 and SAAP-276 incorporated in a controlled-release coating were highly effective against biomaterial-associated infections in vivo (26) demonstrates the potency of SAAPs in tissues.…”

Section: Discussionmentioning

confidence: 99%

The antimicrobial peptide SAAP-148 combats drug-resistant bacteria and biofilms

et al. 2018

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Development of novel antimicrobial agents is a top priority in the fight against multidrug-resistant (MDR) and persistent bacteria. We developed a panel of synthetic antimicrobial and antibiofilm peptides (SAAPs) with enhanced antimicrobial activities compared to the parent peptide, human antimicrobial peptide LL-37. Our lead peptide SAAP-148 was more efficient in killing bacteria under physiological conditions in vitro than many known preclinical- and clinical-phase antimicrobial peptides. SAAP-148 killed MDR pathogens without inducing resistance, prevented biofilm formation, and eliminated established biofilms and persister cells. A single 4-hour treatment with hypromellose ointment containing SAAP-148 completely eradicated acute and established, biofilm-associated infections with methicillin-resistant and MDR from wounded ex vivo human skin and murine skin in vivo. Together, these data demonstrate that SAAP-148 is a promising drug candidate in the battle against antibiotic-resistant bacteria that pose a great threat to human health.

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“…Improved features have been achieved by using antimicrobial polymers for AMP encapsulation. Chitosan has been known for years for its antibacterial properties, which along with its good biocompatibility and biodegradability, make it an excellent candidate for AMPs delivery [82,83]. Chitosan nanoparticles were recently developed for the controlled release of the AMP temporin B (TB) [84].…”

Section: Nanostructures For the Delivery Of Antimicrobial Peptidesmentioning

confidence: 99%

Perspectives on Polymeric Nanostructures for the Therapeutic Application of Antimicrobial Peptides

Sandreschi

Piras

Batoni

et al. 2016

Nanomedicine (Lond.)

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Antimicrobial peptides (AMPs) are a class of promising anti-infective molecules but their therapeutic application is opposed by their poor bioavailability, susceptibility to protease degradation and potential toxicity. The advancement of nanoformulation technologies offers encouraging perspectives for the development of novel therapeutic strategies based on AMPs to treat antibiotic resistant microbial infections. Additionally, the use of polymers endowed per-se with antibacterial properties, stands out as an innovative approach for the development of a new generation of drug delivery systems in which an enhanced antimicrobial action could be obtained by the synergic combination of bioactive polymer matrices and drugs. Herein, the latest AMPs drug delivery research is discussed.

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Chitosan Nanoparticles for the Linear Release of Model Cationic Peptide

Cited by 35 publications

References 31 publications

Antibacterial, Antibiofilm, and Antiadhesive Properties of Different Quaternized Chitosan Derivatives

Antibacterial, Antibiofilm, and Antiadhesive Properties of Different Quaternized Chitosan Derivatives

The antimicrobial peptide SAAP-148 combats drug-resistant bacteria and biofilms

Perspectives on Polymeric Nanostructures for the Therapeutic Application of Antimicrobial Peptides

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