Chitosan nanoparticles enhance the antibacterial activity of the native polymer against bovine mastitis pathogens

Orellano, María Soledad; Isaac, Paula; Breser, María Laura; Bohl, Luciana Paola; Conesa, Agustín; Falcone, R. Darío; Porporatto, Carina

doi:10.1016/j.carbpol.2019.02.016

Cited by 52 publications

(31 citation statements)

References 43 publications

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“…causing bovine mastitis [27,122]. Chitosan in nanoparticle form exhibit higher antimicrobial and anti-biofilm capacity than the native chitosan [123]. Intra-mammary infusion of chitosan can boost up the mammary gland involution and activate host innate immunity, associated with an increase in SCC, bovine serum albumin and lactoferrin concentrations.…”

Section: Othersmentioning

confidence: 99%

Bovine mastitis: risk factors, therapeutic strategies, and alternative treatments — A review

Cheng

Han

2020

Asian-Australas J Anim Sci

273

205

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Bovine mastitis, an inflammation of the mammary gland, is the most common disease of dairy cattle causing economic losses due to reduced yield and poor quality of milk. The etiological agents include a variety of gram-positive and gram-negative bacteria, and can be either contagious (e.g., Staphylococcus aureus, Streptococcus agalactiae, Mycoplasma spp.) or environmental (e.g., Escherichia coli, Enterococcus spp., coagulase-negative Staphylococcus, Streptococcus uberis). Improving sanitation such as enhanced milking hygiene, implementation of post-milking teat disinfection, maintenance of milking machines are general measures to prevent new cases of mastitis, but treatment of active mastitis infection is dependant mainly on antibiotics. However, the extensive use of antibiotics increased concerns about emergence of antibiotic-resistant pathogens and that led the dairy industries to reduce the use of antibiotics. Therefore, alternative therapies for prevention and treatment of bovine mastitis, particularly natural products from plants and animals, have been sought. This review provides an overview of bovine mastitis in the aspects of risk factors, control and treatments, and emerging therapeutic alternatives in the control of bovine mastitis.

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Section: Othersmentioning

confidence: 99%

Bovine mastitis: risk factors, therapeutic strategies, and alternative treatments — A review

Cheng

Han

2020

Asian-Australas J Anim Sci

273

205

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“…For certain bacterial species, the growth stage can impact antibacterial efficiency of nanoparticles. Recently, Orellano et al [82] reported that a notable decrease in colony-forming units (CFUs) of S. aureus was observed after 2 h of co-incubation with CS-NPs, whereas 4 h were required to achieve the same results with CS (p < 0.05). Similar results were observed for S. xylosus.…”

Section: Bacterial Growth Stagementioning

confidence: 99%

Antibacterial Activity of Chitosan Nanoparticles: A Review

2020

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In recent years, nanotechnology has attracted attention in many fields because it has several up-and-coming novel uses. Many researchers have suggested that chitosan nanoparticles (CS-NPs) and their derivatives are one of the best nanomaterials for delivering antibacterial activity. CS-NPs have a broad spectrum of antibacterial activity, but they manifest different inhibitory efficacy against gram-negative (G−) and gram-positive (G+) bacterial species. The mechanism of antibacterial action is an intricate process that varies between G− and G+ bacteria as a result of the differences in cell wall and cell membrane chemistry. In previous studies, greater antibacterial activity was more evident against G− bacteria than G+ bacteria, whereas in some studies G+ bacteria were more sensitive. Researchers predicted that the varied responses of bacteria are caused by the mixed hydrophilicity and negative charge distribution on the bacterial surface. Moreover, its activity depends on a number of variables including bacterial target (i.e., G− or G+ bacteria) and bacterial growth, as well as its concentration, pH, zeta-potential, molecular weight, and degree of acetylation. Therefore, this review examines current research on the mechanisms and factors affecting antibacterial activity, and application of CS-NPs specifically against animal and plant pathogenic bacteria.

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“…Nanoparticles have recently attracted much attention as antimicrobial agents [ 31 ]. It has been reported that NQo have an antimicrobial activity in Staphylococcus aureus isolated from BM [ 32 ]. However, the antimicrobial activity of NQo against BM Pseudomonas pathogen has been poorly explored.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and Antibiofilm Capacity of Chitosan Nanoparticles against Wild Type Strain of Pseudomonas sp. Isolated from Milk of Cows Diagnosed with Bovine Mastitis

et al. 2020

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Bovine mastitis (BM) is the most prevalent bacterial infection in the livestock sector, affecting the dairy industry greatly. The prevention and treatment of this disease is mainly made via antibiotics, but the increasing antimicrobial resistance of pathogens has affected the efficiency of conventional drugs. Pseudomonas sp. is one of the pathogens involved in this infection. The therapeutic rate of cure for this environmental mastitis-causing pathogen is practically zero, regardless of treatment. Biofilm formation has been one of the main virulence mechanisms of Pseudomonas hence presenting resistance to antibiotic therapy. We have manufactured chitosan nanoparticles (NQo) with tripolyphosphate (TPP) using ionotropic gelation. These NQo were confronted against a Pseudomonas sp. strain isolated from milk samples of cows diagnosed with BM, to evaluate their antimicrobial and antibiofilm capacity. The NQo showed great antibacterial effect in the minimum inhibitory concentrations (MIC), minimum bactericidal concentration (MBC) and disk diffusion assays. Using sub lethal concentrations, NQo were tested for inhibition of biofilm formation. The results show that the nanoparticles exhibited biofilm inhibition and were capable of eradicate pre-existing mature biofilm. These findings indicate that the NQo could act as a potential alternative to antibiotic treatment of BM.

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Chitosan nanoparticles enhance the antibacterial activity of the native polymer against bovine mastitis pathogens

Cited by 52 publications

References 43 publications

Bovine mastitis: risk factors, therapeutic strategies, and alternative treatments — A review

Bovine mastitis: risk factors, therapeutic strategies, and alternative treatments — A review

Antibacterial Activity of Chitosan Nanoparticles: A Review

Antimicrobial and Antibiofilm Capacity of Chitosan Nanoparticles against Wild Type Strain of Pseudomonas sp. Isolated from Milk of Cows Diagnosed with Bovine Mastitis

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