Chitosan nanoparticle induced defense responses in fingermillet plants against blast disease caused by Pyricularia grisea (Cke.) Sacc.

Sathiyabama, Muthukrishnan; Manikandan, A.

doi:10.1016/j.carbpol.2016.06.089

Cited by 79 publications

(35 citation statements)

References 44 publications

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“…Infected leaves develop white circular spots with dark/ brown marginal rings, that often coalesce to form larger infected areas, subsequently affected yellow leaves dried, cause significant damage up to 60% 29 . Chitosan NPs have previously been reported as immune modulator through induction of antioxidant/defense enzymes activity in tea and finger millet plants 11 , 19 . Before conducting the pot and field experiments for defense responses and plant growth, in vitro antifungal test was conducted to depict the degree of growth inhibition of Cu-chitosan NPs against C. lunata .…”

Section: Discussionmentioning

confidence: 99%

“…Chitosan, a versatile biomaterial that is of a non-toxic, biocompatible and biodegradable nature, is being exploited in agriculture 5 , 6 . It is well recognized as an antimicrobial 7 , 8 , immuno modulatory 9 – 11 and plant growth promotry agent 12 , 13 . Higher physiological and biochemical responses of chitosan based NPs as compared to bulk chitosan is due to its high surface to volume ratio and surface charge 14 – 16 .…”

Section: Introductionmentioning

confidence: 99%

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Cu-chitosan nanoparticle boost defense responses and plant growth in maize (Zea mays L.)

Choudhary

Kumaraswamy

Kumari

et al. 2017

Sci Rep

271

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In agriculture, search for biopolymer derived materials are in high demand to replace the synthetic agrochemicals. In the present investigation, the efficacy of Cu-chitosan nanoparticles (NPs) to boost defense responses against Curvularia leaf spot (CLS) disease of maize and plant growth promotry activity were evaluated. Cu-chitosan NPs treated plants showed significant defense response through higher activities of antioxidant (superoxide dismutase and peroxidase) and defense enzymes (polyphenol oxidase and phenylalanine ammonia-lyase). Significant control of CLS disease of maize was recorded at 0.04 to 0.16% of Cu-chitosan NPs treatments in pot and 0.12 to 0.16% of NPs treatments in field condition. Further, NPs treatments exhibited growth promotry effect in terms of plant height, stem diameter, root length, root number and chlorophyll content in pot experiments. In field experiment, plant height, ear length, ear weight/plot, grain yield/plot and 100 grain weight were enhanced in NPs treatments. Disease control and enhancement of plant growth was further enlightened through Cu release profile of Cu-chitosan NPs. This is an important development in agriculture nanomaterial research where biodegradable Cu-chitosan NPs are better compatible with biological control as NPs “mimic” the natural elicitation of the plant defense and antioxidant system for disease protection and sustainable growth.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cu-chitosan nanoparticle boost defense responses and plant growth in maize (Zea mays L.)

Choudhary

Kumaraswamy

Kumari

et al. 2017

Sci Rep

271

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“…Cu-chitosan nanoparticles effectively controlled tomato early blight and Fusarium wilt 7 . Chitosan nanoparticles effectively suppressed of rice and finger millet blast fungus Pyricularia grisea 8 , 9 .…”

Section: Introductionmentioning

confidence: 99%

Chitosan nanoparticles having higher degree of acetylation induce resistance against pearl millet downy mildew through nitric oxide generation

Siddaiah

Prasanth

Satyanarayana

et al. 2018

Sci Rep

120

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Downy mildew of pearl millet caused by the biotrophic oomycete Sclerospora graminicola is the most devastating disease which impairs pearl millet production causing huge yield and monetary losses. Chitosan nanoparticles (CNP) were synthesized from low molecular weight chitosan having higher degree of acetylation was evaluated for their efficacy against downy mildew disease of pearl millet caused by Sclerospora graminicola. Laboratory studies showed that CNP seed treatment significantly enhanced pearl millet seed germination percentage and seedling vigor compared to the control. Seed treatment with CNP induced systemic and durable resistance and showed significant downy mildew protection under greenhouse conditions in comparison to the untreated control. Seed treatment with CNP showed changes in gene expression profiles wherein expression of genes of phenylalanine ammonia lyase, peroxidase, polyphenoloxidase, catalase and superoxide dismutase were highly upregulated. CNP treatment resulted in earlier and higher expression of the pathogenesis related proteins PR1 and PR5. Downy mildew protective effect offered by CNP was found to be modulated by nitric oxide and treatment with CNP along with NO inhibitors cPTIO completely abolished the gene expression of defense enzymes and PR proteins. Further, comparative analysis of CNP with Chitosan revealed that the very small dosage of CNP performed at par with recommended dose of Chitosan for downy mildew management.

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“…Leaves and roots of control and treated seedlings were removed at 45 and 90 days and were extracted with Tris-HCl buffer by grinding in a pre-cooled mortar and pestle as described previously (Sathiyabama and Manikandan, 2016). The extract was centrifuged at 10,000 rpm for 10 min at 4°C.…”

Section: Enzyme Assaysmentioning

confidence: 99%

“…The soluble portion was removed and used for enzyme assays. Defense enzymes such as catalase and peroxidase, were assayed as described earlier (Sathiyabama and Manikandan, 2016). Fresh leaves of treated plants were harvested from the selected plants treated with various amendments for estimation of enzymes including, acid phosphatase 45 and 90 th days of experimentation (Weimberg, 1975).…”

Section: Enzyme Assaysmentioning

confidence: 99%

Influence of Some Bioagents and Chitosan Nanoparticles on Controlling Maize Late Wilt and Improving Plants Characteristics

El‐Gazzar

El-Bakery

Ata

2018

Egyptian Journal of Phytopathology

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he high environmental risks of fungicides were a reason for encouraging biological control and its biofactors and recommending its use in the field of plant diseases. Chitosan NPs, Trichoderma viride and mycorrhizae (Glomus mosseae) are one of those factors that have been used in this study separately or in combination to control maize late wilt caused by Cephalosporium maydis. The experiments were conducted during seasons 2017 and 2018 in a greenhouse and infested fields at the Agric. Res. Stat. of Gemmieza. Tested treatments showed a significant decrease in the incidence of late wilt compared to untreated control in either greenhouse or field trials. In this regard, the treatment with chitosan NPs was the best when combined with the mycorrhizae (VAM) which gave the highest effect in controlling late wilt in the greenhouse and field trials followed by treatment with chitosan NPs+ T. viride in addition to the positive effect on plant growth parameters compared to the other treatments. Experiments have also showed that using of T. viride with mycorrhizae together in one treatment led to a law effect on disease control, although their use each alone has a great effect on disease control and improving plant growth. The data also showed that the association of mycorrhizae with maize plants was significantly affected by the infection by C. maydis, in addition to an increase in the activities of peroxidase and catalase enzymes compared to healthy plants. The results obtained from the affected plants revealed that the activities of both enzymes were still less in the plants treated with mycorrhizae than untreated plants. Finally, these results suggest that the use of chitosan NPs in combination with mycorrhizae is one of the methods that can be adopted to achieve the goal of sustainable agriculture in controlling maize late wilt and improve its growth.

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Chitosan nanoparticle induced defense responses in fingermillet plants against blast disease caused by Pyricularia grisea (Cke.) Sacc.

Cited by 79 publications

References 44 publications

Cu-chitosan nanoparticle boost defense responses and plant growth in maize (Zea mays L.)

Cu-chitosan nanoparticle boost defense responses and plant growth in maize (Zea mays L.)

Chitosan nanoparticles having higher degree of acetylation induce resistance against pearl millet downy mildew through nitric oxide generation

Influence of Some Bioagents and Chitosan Nanoparticles on Controlling Maize Late Wilt and Improving Plants Characteristics

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