Chitosan nanoparticle as protein delivery carrier—Systematic examination of fabrication conditions for efficient loading and release

Gan, Quan; Wang, Tao

doi:10.1016/j.colsurfb.2007.04.009

Cited by 612 publications

(449 citation statements)

References 53 publications

Supporting

Mentioning

393

Contrasting

Unclassified

Order By: Relevance

“…Nevertheless, the pH-dependent pattern observed here was not as effective as was in our previous work (Nogueira et al 2013), in which it was used MMW-CS and the surfactant 77KL as bioactive adjuvant in the NP formulation. The faster release achieved here could be attributed to the formulation of NPs with LMW-CS, as it was previously described that the total drug release decreased significantly with increasing CS molecular weight (Gan and Wang 2007). Furthermore, these overall results could be attributed to the lower encapsulation efficiency obtained herein with the surfactant 77KS.…”

Section: In Vitro Release Studysupporting

confidence: 66%

“…This is possibly due to the drug molecules dispersing close to the NP surface. Considering the electrostatic attraction existing between the negatively charged drug and the positively charged polymer, we can assume that the MTX molecules are both adsorbed at the particle surface (Gan and Wang 2007) and entrapped/embedded into CS nano-matrix by hydrogen bonding and hydrophobic forces (Calvo et al 1997). Therefore, the way by which the drug is associated with the carrier (adsorption or encapsulation) might determine its release rate from the matrix.…”

Section: In Vitro Release Studymentioning

confidence: 99%

“…At 480 min, 96 e 100% of MTX was released at pH 6.6 and 5.4, respectively, while only 89% of drug release was reached at pH 7.4. The total drug release at acidic conditions might be attributed to swelling and degradation of the compact NPs (Gan and Wang 2007). Additionally, noteworthy that the accelerated release at acidic conditions might promote an improved drug delivery in the acidic extracellular pH of tumors (pHe ~ 6.6) (Na et al 2003).…”

Section: In Vitro Release Studymentioning

confidence: 99%

See 2 more Smart Citations

Inclusion of a pH-responsive amino acid-based amphiphile in methotrexate-loaded chitosan nanoparticles as a delivery strategy in cancer therapy

et al. 2015

View full text Add to dashboard Cite

The encapsulation of antitumor drugs in nanosized systems with pH-sensitive behavior is a promissing approach that may enhance the success of chemotherapy in many cancers. The nanocarrier dependence on pH might trigger an efficient delivery of the encapsulated drug both in the acidic extracellular environment of tumors and, especially, in the intracellular compartments through disruption of endosomal membrane. In this context, here we reported the preparation of chitosan-based nanoparticles encapsulating methotrexate as a model drug (MTX-CS-NPs), which comprises the incorporation of an amino acid-based amphiphile with pHresponsive properties (77KS) on the ionotropic complexation process. The presence of 77KS clearly gives a pH-sensitive behavior to NPs, which allowed accelerated release of MTX with decreasing pH as well as pH-dependent membrane-lytic activity. This latter performance demonstrates the potential of these NPs to facilitate cytosolic delivery of endocytosed materials.Outstandingly, the cytotoxicity of MTX-loaded CS-NPs was higher than free drug to MCF-7 tumor cells and, to a lesser extent, to HeLa cells. Based on the overall results, MTX-CS-NPs modified with the pH-senstive surfactant 77KS could be potentially useful as a carrier system for intracellular drug delivery and, thus, a promising targeting anticancer chemotherapeutic agent.

show abstract

Section: In Vitro Release Studysupporting

confidence: 66%

Section: In Vitro Release Studymentioning

confidence: 99%

Section: In Vitro Release Studymentioning

confidence: 99%

See 1 more Smart Citation

Inclusion of a pH-responsive amino acid-based amphiphile in methotrexate-loaded chitosan nanoparticles as a delivery strategy in cancer therapy

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Enzymes immobilization onto carriers has been extensively studied and applied in many fields, such as biocatalysts (Hou et al, 2007;Li et al, 2010) medical devices (Liang et al, 2000;Lao et al, 2008), drug delivery systems (Gan and Wang, 2007;Shi et al, 2011a) and biosensor (Ley et al, 2011;Samanta and Sarkar, 2011). Several carriers such as synthetic organic polymers (e.g., Eupergit C and polyurethane), biopolymers (e.g., alginate), hydrogels (e.g., Polyvinyl alcohol), smart polymers (e.g., poly-N-isopropylacrylamide) and inorganic supports (e.g., alumina, silica and zeolites) have been used in immobilization of enzymes (Katchalski-Katzir and Kraemer, 2000;Kirk and Christensen, 2002;Temino et al, 2005;Lutz et al, 2006;Awang et al, 2007;Sheldon, 2007).…”

Section: Immobilized Enzyme Supportsmentioning

confidence: 99%

Potential Applications of Chitosan Nanoparticles as Novel Support in Enzyme Immobilization

Malmiri

Jahanian

Berenjian

2012

American Journal of Biochemistry and Biotechnology

View full text Add to dashboard Cite

Chitosan is an attractive natural biopolymer from renewable resources with the presence of reactive amino and hydroxyl functional groups in its structure. Due to the good biocompatibility of chitosan, it can be used in magnetic-field assisted drug delivery, enzyme or cell immobilization and many other industrial applications. In the past decade, nanotechnology has been a considerable research interest in the area of preparation of immobilized enzyme carriers. This study looks at characteristics and applications of chitosan and chitosan nanoparticles and their potentials as suitable supports for enzyme immobilization. Results indicated that activity of immobilized enzymes and performance of enzyme immobilization onto chitosan nanoparticles are higher than chitosan macro and microparticles. As compared to other biopolymers nanoparticles, application of chitosan nanoparticles to immobilize enzymes strongly increases stability of immobilized enzymes and their easy separability from the reaction mixture at the end of the biochemical process.

show abstract

“…Several strategies involving nanosized carriers for photosensitizer delivery such as liposomes, polymeric micelles, metal-based or silica-based nanoparticles have already been explored [15][16][17][18]. The preparation of chitosan-based hydrophilic nanogels has been widely described in the literature, employing penta sodium triphosphate (PSTP) as the complexing anionic molecule for the polycation chitosan [19][20][21][22][23][24][25]. Chitosan-based nanogels have been studied as carriers of oligonucleotides in gene therapy, for mucosal vaccination, in tissue engineering or drug delivery [26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Chitosan-based nanogels for selective delivery of photosensitizers to macrophages and improved retention in and therapy of articular joints

Schmitt

Lagopoulos

Käuper³

et al. 2010

Journal of Controlled Release

111

View full text Add to dashboard Cite

Macrophages play key roles in inflammatory disorders. Therefore, they are targets of treatments aiming at their local destruction in inflammation sites. However, injection of low molecular mass therapeutics, including photosensitizers, in inflamed joints results in their rapid efflux out of the joints, and poor therapeutic index. To improve selective uptake and increase retention of therapeutics in inflamed tissues, hydrophilic nanogels based on chitosan, of which surface was decorated with hyaluronate and which were loaded with one of three different anionic photosensitizers were developed. Optimal uptake of these functionalized nanogels by murine RAW 264.7 or human THP-1 macrophages as models was achieved after b 4 h incubation, whereas only negligible uptake by murine fibroblasts used as control cells was observed. The uptake by cells and the intracellular localization of the photosensitizers, of the fluorescein-tagged chitosan and of the rhodamine-tagged hyaluronate were confirmed by fluorescence microscopy. Photodynamic experiments revealed good cell photocytotoxicity of the photosensitizers entrapped in the nanogels. In a mouse model of rheumatoid arthritis, injection of free photosensitizers resulted in their rapid clearance from the joints, while nanogel-encapsulated photosensitizers were retained in the inflamed joints over a longer period of time. The photodynamic treatment of the inflamed joints resulted in a reduction of inflammation comparable to a standard corticoid treatment. Thus, hyaluronate-chitosan nanogels encapsulating therapeutic agents are promising materials for the targeted delivery to macrophages and long-term retention of therapeutics in leaky inflamed articular joints.

show abstract

Chitosan nanoparticle as protein delivery carrier—Systematic examination of fabrication conditions for efficient loading and release

Cited by 612 publications

References 53 publications

Inclusion of a pH-responsive amino acid-based amphiphile in methotrexate-loaded chitosan nanoparticles as a delivery strategy in cancer therapy

Inclusion of a pH-responsive amino acid-based amphiphile in methotrexate-loaded chitosan nanoparticles as a delivery strategy in cancer therapy

Potential Applications of Chitosan Nanoparticles as Novel Support in Enzyme Immobilization

Chitosan-based nanogels for selective delivery of photosensitizers to macrophages and improved retention in and therapy of articular joints

Contact Info

Product

Resources

About