Chitosan modified poly(l-lactide) microspheres as cell microcarriers for cartilage tissue engineering

Lao, Lihong; Tan, Huaping; Wang, Yingjun; Gao, Changyou

doi:10.1016/j.colsurfb.2008.06.014

Cited by 119 publications

(85 citation statements)

References 29 publications

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“…Promising techniques include surface or bulk modification of the biomaterial composition (composite scaffolds [26,27], surface modification [28,29] and coating with bioactive molecules [30]). Other upcoming approaches are nanofiber technologies [31] and designed materials [32,33].…”

Section: Introductionmentioning

confidence: 99%

The influence of scaffold architecture on chondrocyte distribution and behavior in matrix-associated chondrocyte transplantation grafts

et al. 2011

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Section: Introductionmentioning

confidence: 99%

The influence of scaffold architecture on chondrocyte distribution and behavior in matrix-associated chondrocyte transplantation grafts

et al. 2011

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“…Polysaccharides outside the cell membrane are very important signal molecules. Chitosan can mimic ECM and act as polysaccharides to help cells to adjust their growth and function [44]. Besides, chitosan is a very good cation absorber [45] and the membrane acts as a positively charged center in solution.…”

Section: Degradationmentioning

confidence: 99%

Biodegradable electrospun PLLA/chitosan membrane as guided tissue regeneration membrane for treating periodontitis

et al. 2013

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This paper explores the application potential of a biodegradable PLLA/chitosan electrospun composite membrane for guided periodontal tissue regeneration which in addition serves as a fibroblast barrier. Electrospinning was applied to fabricate the PLLA membrane and aminolysis method was applied to graft chitosan on its surface. The morphology of the PLLA/chitosan membrane was observed by SEM. The surface chemical composition was analyzed by XPS. The appearance of N 1s peak in XPS demonstrated the successful grafting of chitosan on the PLLA electrospin membrane. After the modification, the water contact angle decreased from 136.9 ± 2.18°to 117.0 ± 2.10°, representing an improved hydrophilicity of the membrane. The bioactivity of the membrane was analyzed by XPS after soaking in SBF. The deposits had a Ca/P ratio of 1.6, indicating the hydroxyapatite formation on PLLA/chitosan membrane. The degradation rate was determined by measuring mass loss after immersion in PBS at different time periods. Compared to pure PLLA electrospun membrane which was almost nondegradable, the degradation rate of PLLA/chitosan composite membrane was up to 20 % in 6 weeks while maintaining its basic architecture to keep supporting the regenerated tissue. Live-dead cell staining of MC3T3 E1 cells cultured on the surface of the membrane showed a good biocompatibility of the PLLA/chitosan membrane. Furthermore, fibroblast cell line NIH 3T3 was cultured on surface of the membrane for the evaluation of cell penetration. The result demonstrated that the membrane worked as a fibroblast barrier to minimize the unfavorable effect of fibroblasts on periodontal tissue regeneration. Therefore, this electrospun PLLA/chitosan composite membrane has more potential for clinical application compared to old generation regeneration membrane with both suitable degradation rate and non-fibroblast penetration property.

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“…Thus, the combination of microspheres with cells ex vivo prior to implantation can lead to enhanced tissue regeneration. Other polymers could also be blended with poly(α-hydroxy acids) to fabricate microcarriers for cells [67,[113][114]. Other synthetic injectable platforms include polycaprolactone particles featuring cell-adhesive polydopamine for human neural stem cell attachment [115].…”

Section: Injectable Microspheres For Tissue Engineeringmentioning

confidence: 99%

Nanostructured Injectable Cell Microcarriers for Tissue Regeneration

Zhang

Eyster

Peter

2016

Nanomedicine (Lond.)

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Biodegradable polymer microspheres have emerged as cell carriers for the regeneration and repair of irregularly shaped tissue defects due to their injectability, controllable biodegradability and capacity for drug incorporation and release. Notably, recent advances in nanotechnology allowed the manipulation of the physical and chemical properties of the microspheres at the nanoscale, creating nanostructured microspheres mimicking the composition and/or structure of natural extracellular matrix. These nanostructured microspheres, including nanocomposite microspheres and nanofibrous microspheres, have been employed as cell carriers for tissue regeneration. They enhance cell attachment and proliferation, promote positive cell-carrier interactions and facilitate stem cell differentiation for target tissue regeneration. This review highlights the recent advances in nanostructured microspheres that are employed as injectable, biomimetic and cell-instructive cell carriers.

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Chitosan modified poly(l-lactide) microspheres as cell microcarriers for cartilage tissue engineering

Cited by 119 publications

References 29 publications

The influence of scaffold architecture on chondrocyte distribution and behavior in matrix-associated chondrocyte transplantation grafts

The influence of scaffold architecture on chondrocyte distribution and behavior in matrix-associated chondrocyte transplantation grafts

Biodegradable electrospun PLLA/chitosan membrane as guided tissue regeneration membrane for treating periodontitis

Nanostructured Injectable Cell Microcarriers for Tissue Regeneration

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