Chitosan modified graphite electrodes developed for electrochemical monitoring of interaction between daunorubicin and DNA

Çongur, Gülşah; Ekşin, Ece; Erdem, Arzum

doi:10.1016/j.sbsr.2018.100255

Cited by 23 publications

(9 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Since the amino groups in chitosan have a p K a value of ∼6.5 at 298 K, they are protonated at acidic pH and the resulting cationic groups in principle can bind anions of Mo(VI). In addition, chitosan is known to show good adhesion to electrodes and excellent film forming ability. , …”

Section: Resultsmentioning

confidence: 99%

“…In addition, chitosan is known to show good adhesion to electrodes and excellent film forming ability. 34,35 Molybdate(VI) was immobilized on GC macroelectrodes by drop-casting a certain amount of an AMT-CS suspension dissolved in 0.1 M acetic acid to create a Mo(VI)-chitosanmodified electrode (AMT-CS-GCE). Then to explore the electrochemical response of AMT-CS-GCE, three GC electrodes after different treatments were compared: (1) an unmodified GCE after polishing, (2) chitosan immobilized on a bare GCE; and (3) an AMT-CS-GCE as described in the Experimental Section.…”

Section: Speciation Of Mo(vi) In Aqueous Solution the Equilibration O...mentioning

confidence: 99%

See 1 more Smart Citation

Amperometric Environmental Phosphate Sensors

et al. 2021

ACS Sens.

View full text Add to dashboard Cite

We report a novel amperometric sensor for aqueous phosphate ions in freshwater systems based on the reductive square wave voltammetry of molybdate(VI) anions immobilized within a chitosan matrix deposited on a glassy carbon electrode. A sensitivity of 4.4 ± 0.1 μA/μM was realized together with a LOD of 0.15 μM. The sensor was insensitive to chloride and nitrate ions below a threshold concentration of 1.0 mM. Analytical measurements were successfully made in authentic samples of tap and pond water.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Speciation Of Mo(vi) In Aqueous Solution the Equilibration O...mentioning

confidence: 99%

Amperometric Environmental Phosphate Sensors

et al. 2021

ACS Sens.

View full text Add to dashboard Cite

show abstract

“…While SPCE was used in this study for the development of an electrochemical genosensor, Eksin and co-workers [ 45 ] instead used chitosan as the material for the electrode. Although chitosan has been reported as a good natural polysaccharide for biosensor preparation [ 70 , 71 , 72 ], the use of screen-printed electrodes (SPEs) in biosensors has also been reported to confer the advantages of adaptability, low-cost, ease of mass production, and selective specificity for target analytes [ 44 , 73 , 74 , 75 ]. Additionally, SPEs can be easily customized in terms of their shape, substrate, and dimension, which provides for easy selectivity and calibration of SPE-based biosensors.…”

Section: Discussionmentioning

confidence: 99%

Sequence-Specific Electrochemical Genosensor for Rapid Detection of blaOXA-51-like Gene in Acinetobacter baumannii

et al. 2022

View full text Add to dashboard Cite

Acinetobacter baumannii (A. baumannii) are phenotypically indistinguishable from the Acinetobacter calcoaceticus–A. baumannii (ACB) complex members using routine laboratory methods. Early diagnosis plays an important role in controlling A. baumannii infections and this could be assisted by the development of a rapid, yet sensitive diagnostic test. In this study, we developed an enzyme-based electrochemical genosensor for asymmetric PCR (aPCR) amplicon detection of the blaOXA-51-like gene in A. baumannii. A. baumannii blaOXA-51-like gene PCR primers were designed, having the reverse primer modified at the 5′ end with FAM. A blaOXA-51-like gene sequence-specific biotin labelled capture probe was designed and immobilized using a synthetic oligomer (FAM-labelled) deposited on the working electrode of a streptavidin-modified, screen-printed carbon electrode (SPCE). The zot gene was used as an internal control with biotin and FAM labelled as forward and reverse primers, respectively. The blaOXA-51-like gene was amplified using asymmetric PCR (aPCR) to generate single-stranded amplicons that were detected using the designed SPCE. The amperometric current response was detected with a peroxidase-conjugated, anti-fluorescein antibody. The assay was tested using reference and clinical A. baumannii strains and other nosocomial bacteria. The analytical sensitivity of the assay at the genomic level and bacterial cell level was 0.5 pg/mL (1.443 µA) and 103 CFU/mL, respectively. The assay was 100% specific and sensitive for A. baumannii. Based on accelerated stability performance, the developed genosensor was stable for 1.6 years when stored at 4 °C and up to 28 days at >25 °C. The developed electrochemical genosensor is specific and sensitive and could be useful for rapid, accurate diagnosis of A. baumannii infections even in temperate regions.

show abstract

“…The stationary working electrode is composed of various types of electrode materials. The most common are glassy carbon electrodes (GCEs) [ 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 ], pencil graphite electrodes (PGEs) [ 50 , 51 , 52 , 53 , 54 , 55 , 56 ], carbon paste electrodes (CPEs) [ 57 , 58 ], hanging mercury drop electrodes (HMDEs) [ 59 ], platinum electrodes [ 60 ], and gold electrodes [ 61 , 62 ]. These electrodes can be used either in the bare form [ 44 , 54 , 63 ] or modified with a layer capable of increasing their sensitivity [ 50 , 51 , 52 , 53 , 55 , 56 ].…”

Section: Electrochemical Approach To Dna–drug Interaction Descriptionmentioning

confidence: 99%

What Can Electrochemical Methods Offer in Determining DNA–Drug Interactions?

2021

View full text Add to dashboard Cite

The interactions of compounds with DNA have been studied since the recognition of the role of nucleic acid in organisms. The design of molecules which specifically interact with DNA sequences allows for the control of the gene expression. Determining the type and strength of such interaction is an indispensable element of pharmaceutical studies. Cognition of the therapeutic action mechanisms is particularly important for designing new drugs. Owing to their sensitivity, simplicity, and low costs, electrochemical methods are increasingly used for this type of research. Compared to other techniques, they require a small number of samples and are characterized by a high reliability. These methods can provide information about the type of interaction and the binding strength, as well as the damage caused by biologically active molecules targeting the cellular DNA. This review paper summarizes the various electrochemical approaches used for the study of the interactions between pharmaceuticals and DNA. The main focus is on the papers from the last decade, with particular attention on the voltammetric techniques. The most preferred experimental approaches, the electrode materials and the new methods of modification are presented. The data on the detection ranges, the binding modes and the binding constant values of pharmaceuticals are summarized. Both the importance of the presented research and the importance of future prospects are discussed.

show abstract

Chitosan modified graphite electrodes developed for electrochemical monitoring of interaction between daunorubicin and DNA

Cited by 23 publications

References 33 publications

Amperometric Environmental Phosphate Sensors

Amperometric Environmental Phosphate Sensors

Sequence-Specific Electrochemical Genosensor for Rapid Detection of blaOXA-51-like Gene in Acinetobacter baumannii

What Can Electrochemical Methods Offer in Determining DNA–Drug Interactions?

Contact Info

Product

Resources

About