Chitosan influence on glucose and calcium availability from yogurt: In vitro comparative study with plants fibre

Rodríguez, María Susana; Montero, Mirta; Staffolo, Marina Dello; Martino, Miriam Nora; Bevilacqua, A.E.; Albertengo, Liliana

doi:10.1016/j.carbpol.2008.04.046

Cited by 23 publications

(21 citation statements)

References 27 publications

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“…However availability reduction responses, between insoluble fibers (wheat and bamboo) and soluble ones (inulin and psyllium plantago), have no significant differences (p < 0.05) by Tukey's test. Again, like results obtained with glucose, this study demonstrated that the chitosan effect is more pronounced and higher than for the other studies [138]. To study iron retention percentages by the fibers tested in the present study, the introduction of cellulose dialysis tubes in the digestive chemical experimental model is utilised.…”

Section: Digestive Chemical Model and Glucose Calcium And Iron Retensupporting

confidence: 72%

“…It was obtained from crustacean chitin in the Laboratorio de Investigación Básica y Aplicada en Quitina (LIBAQ-INQUISUR-CONICET), Universidad Nacional del Sur, Bahía Blanca, Argentina. Chitin firstly was isolated from shrimp (Pleoticus mülleri) waste by the process that was described in our previous work [138]. Chitosan was prepared directly by heterogeneous deacetylation of chitin with 50% (w/w) NaOH.…”

Section: Dietary Fibers Employedmentioning

confidence: 99%

“…In calcium availability studies the digestive mimicking was done without the addition of exogenous calcium because yoghurt is a source of calcium in the diet [138]. To evaluate the interactions between the fibers and iron, 0.8% (w/w) of ferrous sulfate was added to yoghurt samples with each type of fiber [139].…”

Section: Yoghurt Preparationmentioning

confidence: 99%

“…Glucose and calcium amounts, determined by this way, represent the bioavailability fraction of those nutrients. A control without fibers was made to consider glucose and calcium 100% availability [138].…”

Section: Digestive Chemical Experimental Modelmentioning

confidence: 99%

“…Assays were carried directly in spectrophotometer test tubes: 50 lL Cpx were mixed with a plastic rod and absorbance was read in spectrophotometer (Spectronic 20 Genesys TM, Spectronic Instrument, USA) at 570 nm (internal blank), then 20 mL of each digestive mimicking sample were added, immediately mixed and read after 10 min. A standard curve was developed [138].…”

Section: Analytical Techniquesmentioning

confidence: 99%

See 4 more Smart Citations

Dietary Fiber and Availability of Nutrients: A Case Study on Yoghurt as a Food Model

Staffolo¹,

Bevilacqua²,

Rodríguez³

et al. 2012

The Complex World of Polysaccharides

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Section: Digestive Chemical Model and Glucose Calcium And Iron Retensupporting

confidence: 72%

Section: Dietary Fibers Employedmentioning

confidence: 99%

Section: Yoghurt Preparationmentioning

confidence: 99%

Section: Digestive Chemical Experimental Modelmentioning

confidence: 99%

Section: Analytical Techniquesmentioning

confidence: 99%

See 3 more Smart Citations

Dietary Fiber and Availability of Nutrients: A Case Study on Yoghurt as a Food Model

Staffolo¹,

Bevilacqua²,

Rodríguez³

et al. 2012

The Complex World of Polysaccharides

Self Cite

View full text Add to dashboard Cite

Marine Nutraceuticals

Himaya¹,

Kim²

2015

Hb25_Springer Handbook of Marine Biotechnology

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Evidence on antimicrobial properties and mode of action of a chitosan obtained from crustacean exoskeletons on Pseudomonas syringae pv. tomato DC3000

Mansilla

Albertengo

Rodríguez

et al. 2013

Appl Microbiol Biotechnol

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Pseudomonas syringae pv. tomato DC3000 (Pto DC3000) causes bacterial speck of tomato, a widely spread disease that causes significant economical losses worldwide. It is representative of many bacterial plant diseases for which effective controls are still needed. Despite the antimicrobial properties of chitosan has been previously described in phytopathogenic fungi, its action on bacteria is still poorly explored. In this work, we report that the chitosan isolated from shrimp exoskeletons (70 kDa and 78 % deacetylation degree) exerts cell damage on Pto DC3000. Chitosan inhibited Pto DC3000 bacterial growth depending on its concentration, medium-pH, and presence of metal ion (Mg(+2)). Biochemical and cellular changes resulting in cell aggregation and impaired bacterial growth were also viewed. In vivo studies using fluorescent probes showed cell aggregation, increase in membrane permeability, and cell death, suggesting the chitosan antibacterial activity is due to its interaction as a polycation with Pto DC3000 membranes. Transmission electron microscopic analysis revealed that chitosan also caused morphological changes and damage in bacterial surfaces. Also, the disease incidence in tomato inoculated with Pto DC3000 was significantly reduced in chitosan pretreated seedlings, revealing a promising action of chitosan as nontoxic biopesticide in tomato plants. Indeed, a wider comprehensive knowledge of the mechanism of action of chitosan in phytopathogenic bacterial cells will increase the chances of its successful application to the control of spread disease in plants.

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Chitosan influence on glucose and calcium availability from yogurt: In vitro comparative study with plants fibre

Cited by 23 publications

References 27 publications

Dietary Fiber and Availability of Nutrients: A Case Study on Yoghurt as a Food Model

Dietary Fiber and Availability of Nutrients: A Case Study on Yoghurt as a Food Model

Marine Nutraceuticals

Evidence on antimicrobial properties and mode of action of a chitosan obtained from crustacean exoskeletons on Pseudomonas syringae pv. tomato DC3000

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