Chitosan induces different l-arginine metabolic pathways in resting and inflammatory macrophages

Porporatto, Carina; Bianco, Ismael D.; Riera, Clelia M.; Correa, Silvia G.

doi:10.1016/s0006-291x(03)00579-5

Cited by 96 publications

(57 citation statements)

References 25 publications

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“…Peluso et al (1994) also demonstrated that chitosan could stimulate the macrophages in the rats and increase NO secretion. Porporatto et al (2003) have shown the similar result that 0.05 % ~ 0.1 % chitosan significantly enhanced the content of NO and the expression of iNOS mRNA in rat macrophages. Shi (2004) also showed that chitosan supplementation had better effects on the splenocyte of broilers iNOS and NO production in dose-dependent manner, but its effect on iNOS gene expression had not been studied.…”

Section: Discussionsupporting

confidence: 62%

“…iNOS is responsible for generating high levels of NO in activated macrophages, and its expression is essential for the functions of NO in the regulation of immune responses (Xing and Schat 2000). Porporatto et al (2003) found that chitosan significantly enhanced the NO content and iNOS expression in rat macrophages. This implied that enhanced immune functions by chitosan are associated with the activation and strengthening of secretory functions of macrophages and increased expression of iNOS.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Chitosan on the Secretion of Cytokines and Expression of Inducible Nitric Oxide Synthase mRNA in Peritoneal Macrophages of Broiler Chicken

Shi

Yan

et al. 2014

Braz. arch. biol. technol.

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Section: Discussionsupporting

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Effects of Chitosan on the Secretion of Cytokines and Expression of Inducible Nitric Oxide Synthase mRNA in Peritoneal Macrophages of Broiler Chicken

Shi

Yan

et al. 2014

Braz. arch. biol. technol.

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“…The findings of the current research were consistent with those of previous studies. It was reported that the strong stimulatory activity of chitosan has been mainly attributed to the N-acetyl-d-glucosamine residues and its effect could be mediated by combined action with a macrophage mannose specific receptor (Porporatto et al 2003;Feng et al 2004). Zhao (2004) indicated that macrophages activated by oligochitosan in- Porporatto et al (2005) found that chitosan could be absorbed by the antigen-presenting cells and profoundly affected immunity by activating the T cell in the intestinal mucosa of rats.…”

Section: Discussionmentioning

confidence: 99%

“…Results of the in vitro trial showed that chitosan significantly enhanced the NO content and iNOS expression in rat macrophages (Porporatto et al 2003). However, Yang et al (2010) indicated that chitosan markedly inhibited the LPS-induced NO production of RAW 264.7 cells.…”

Section: Introductionmentioning

confidence: 97%

Effects of chitosan on nitric oxide production and inducible nitric oxide synthase activity and mRNA expression in weaned piglets

Li¹,

Shi²,

Yan³

et al. 2015

CZECH J ANIM SCI

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ABSTRACT:The effects of chitosan on nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) activity and gene expression in vivo or vitro were investigated in weaned piglets. In vivo, 180 weaned piglets were assigned to five dietary treatments with six replicates. The piglets were fed on a basal diet supplemented with 0 (control), 100, 500, 1000, and 2000 mg chitosan/kg feed, respectively. In vitro, the peripheral blood mononuclear cells (PBMCs) from a weaned piglet were cultured respectively with 0 (control), 40, 80, 160, and 320 µg chitosan/ml medium. Results showed that serum NO concentrations on days 14 and 28 and iNOS activity on day 28 were quadratically improved with increasing chitosan dose (P < 0.05). The iNOS mRNA expressions were linearly or quadratically enhanced in the duodenum on day 28, and were improved quadratically in the jejunum on days 14 and 28 and in the ileum on day 28 (P < 0.01). In vitro, the NO concentrations, iNOS activity, and mRNA expression in unstimulated PBMCs were quadratically enhanced by chitosan, but the improvement of NO concentrations and iNOS activity by chitosan were markedly inhibited by N- (3-[aminomethyl] benzyl) acetamidine (1400w) (P < 0.05). Moreover, the increase of NO concentrations, iNOS activity, and mRNA expression in PBMCs induced by lipopolysaccharide (LPS) were suppressed significantly by chitosan (P < 0.05). The results indicated that the NO concentrations, iNOS activity, and mRNA expression in piglets were increased by feeding chitosan in a dosedependent manner. In addition, chitosan improved the NO production in unstimulated PBMCs but inhibited its production in LPS-induced cells, which exerted bidirectional regulatory effects on the NO production via modulated iNOS activity and mRNA expression.

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“…A quitosana e respetivos oligômeros inibem a ativação e a expressão da metaloproteinase-2 da matriz em fibroblastos humanos (Kim e Kim, 2006), estimulando a cicatrização dos tecidos. O efeito imunoestimulador de derivados de quitosana tem sido atribuído à presença dos grupos N-acetil-D-glucosamina (Porporatto et al, 2003), que atuam estimulando as células inflamatórias como os neutrófilos PMN, macrófagos e fibroblastos. A quitosana promove a produção do fator de transformação do crescimento β (TGF-β) e do fator de crescimento derivado de plaquetas por monócitos humanos derivados de macrófagos (Ueno et al, 2001a) envolvidos na resposta inflamatória.…”

Section: Anti-inflamatóriaunclassified

A quitosana como biomaterial odontológico: estado da arte

Tavaria

Costa

Pina‐Vaz

et al. 2013

RBEB

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Resumo A impossibilidade de erradicar completamente os microrganismos da cavidade oral ou dos canais radiculares estimula a procura de novos materiais que permitam cada vez mais eficazmente, controlar ou prevenir o aparecimento de infeções. A quitosana é um biopolímero com uma estrutura química e características únicas que permitem que apresente excelente biocompatibilidade e que seja facilmente processado. A panóplia de aplicações é tão fascinante como ainda mal conhecida. A constatação do potencial e do valor industrial deste biopolímero conduziu à sua utilização em muitas aplicações de interesse tecnológico e, de uma forma acentuada, na área biomédica. As suas propriedades anti-oxidantes, anti-microbianas, anti-inflamatórias, cicatrizante e de inibição da formação de biofilmes tornam a sua aplicação na odontologia particularmente interessante. Com este trabalho pretendeu-se rever a literatura existente sobre aplicações da quitosana na área odontológica. Palavras-chave

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Chitosan induces different l-arginine metabolic pathways in resting and inflammatory macrophages

Cited by 96 publications

References 25 publications

Effects of Chitosan on the Secretion of Cytokines and Expression of Inducible Nitric Oxide Synthase mRNA in Peritoneal Macrophages of Broiler Chicken

Effects of Chitosan on the Secretion of Cytokines and Expression of Inducible Nitric Oxide Synthase mRNA in Peritoneal Macrophages of Broiler Chicken

Effects of chitosan on nitric oxide production and inducible nitric oxide synthase activity and mRNA expression in weaned piglets

A quitosana como biomaterial odontológico: estado da arte

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