Chitosan hydrogel formation using laccase activated phenolics as cross-linkers

Huber, Daniela; Tegl, Gregor; Baumann, Martina; Sommer, Eva; Gorji, Elham Ghorbani; Borth, Nicole; Schleining, Gerhard; Nyanhongo, Gibson S.; Guebitz, Georg M.

doi:10.1016/j.carbpol.2016.10.012

Cited by 83 publications

(30 citation statements)

References 63 publications

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“…These cross linkers do not only enable cross linking of multiple compounds but also show antioxidant properties. The study further proved the biocompatibility of these materials in cytotoxicity test on mice fibroblasts [16].…”

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confidence: 58%

The chemo enzymatic functionalization of chitosan zeolite particles provides antioxidant and antimicrobial properties

Tegl

Stagl

Mensah

et al. 2018

Engineering in Life Sciences

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Silicate‐based microporous materials like zeolites are nano enabled particles and used for various applications including pharmaceutical formulations. This study reports on the chemo‐enzymatic functionalization of chitosan‐zeolite particles (CTS‐zeolites) with caffeic acid (CA) and glucose oxidase (GOX) to impart combined antioxidant and antimicrobial properties. CA was grafted on the chitosan moieties by using laccase generating stable particles (zeta potential –36.7 mV) of high antioxidant activity (44% DPPH inhibition). GOX was immobilized both on CTS‐zeolites and on CA modified CTS‐zeolites and creating a hydrogen peroxide generation system continuously and in‐situ producing this oxidative and antimicrobial agent. The system prevented bacterial growth of E. coli and S. aureus over 24 h whereby a steady‐state concentration of around 60 μM hydrogen peroxide in the culture medium was observed. CA and GOX functionalized CTS‐zeolite particles additionally showed combinatorial antioxidant and antimicrobial properties providing a powerful bioactive system for medical applications. These particles proved their suitability for incorporation in bioactive formulations which could be used, inter alia, for topical wound treatments.

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confidence: 58%

The chemo enzymatic functionalization of chitosan zeolite particles provides antioxidant and antimicrobial properties

Tegl

Stagl

Mensah

et al. 2018

Engineering in Life Sciences

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“…Similar materials were also obtained by the laccase oxidation of fenugreek gum [176]. Chitosan hydrogels with laccase-oxidized phenolics as crosslinkers showed improved swelling properties and suitable biocompatibility as well as conductive gels based on chitosan and hyaluronic acid [177], (CN104725657A).…”

Section: Nanobiotechnology and Biomedicinementioning

confidence: 84%

Applications of Microbial Laccases: Patent Review of the Past Decade (2009–2019)

et al. 2019

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There is a high number of well characterized, commercially available laccases with different redox potentials and low substrate specificity, which in turn makes them attractive for a vast array of biotechnological applications. Laccases operate as batteries, storing electrons from individual substrate oxidation reactions to reduce molecular oxygen, releasing water as the only by-product. Due to society’s increasing environmental awareness and the global intensification of bio-based economies, the biotechnological industry is also expanding. Enzymes such as laccases are seen as a better alternative for use in the wood, paper, textile, and food industries, and they are being applied as biocatalysts, biosensors, and biofuel cells. Almost 140 years from the first description of laccase, industrial implementations of these enzymes still remain scarce in comparison to their potential, which is mostly due to high production costs and the limited control of the enzymatic reaction side product(s). This review summarizes the laccase applications in the last decade, focusing on the published patents during this period.

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“…Phenolic components obtained from different kinds of plants can interact (intermolecular interactions) with hydroxyl and free amine groups of [264,265] that reduce the solubility of chitosan films: by hydrogen-bonding, electrostatic attraction (ionic complexations in acidic conditions) [266] between the anthocyanins and chitosan, and even through ester linkage [253,267]. For example, a small amount of purple-fleshed sweet potato extract (PSPE) (5 wt % on chitosan basis) could establish physical interactions with chitosan molecules and act as bridges among different chitosan chains [205,220].…”

Section: Phenols From Plant Extracts As Crosslinkers For the Chitosanmentioning

confidence: 99%

Vegetable Additives in Food Packaging Polymeric Materials

Munteanu

Vasile

2019

Polymers

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Plants are the most abundant bioresources, providing valuable materials that can be used as additives in polymeric materials, such as lignocellulosic fibers, nano-cellulose, or lignin, as well as plant extracts containing bioactive phenolic and flavonoid compounds used in the healthcare, pharmaceutical, cosmetic, and nutraceutical industries. The incorporation of additives into polymeric materials improves their properties to make them suitable for multiple applications. Efforts are made to incorporate into the raw polymers various natural biobased and biodegradable additives with a low environmental fingerprint, such as by-products, biomass, plant extracts, etc. In this review we will illustrate in the first part recent examples of lignocellulosic materials, lignin, and nano-cellulose as reinforcements or fillers in various polymer matrices and in the second part various applications of plant extracts as active ingredients in food packaging materials based on polysaccharide matrices (chitosan/starch/alginate).In this review various recent applications of plant-based additives (lignocellulosic fibers/nano-cellulose as well as bioactive plant extracts) as reinforcements and active ingredients in food packaging materials are illustrated. Natural polysaccharide biopolymers (such as chitosan/starch/alginate) are nontoxic, biodegradable, biocompatible, and largely used in food packaging: Chitosan is known for its broad antimicrobial activity and its excellent film-forming properties; alginates have good film-forming properties, retain moisture, reduce microbial counts, and retard oxidative off-flavors; and starch is also particularly important for its cheap price and its frequency in nature. For these reasons, this review will present applications of plant extracts as active ingredients in natural polysaccharide biopolymers: chitosan/starch/alginate. Classification of Natural Biodegradable Polymers and AdditivesNatural biodegradable polymers and additives are polymers formed naturally by the living organisms by enzyme-catalyzed reactions and reactions of chain growth from monomers which are formed inside the cells by complex metabolic processes [5].Natural additives can be high molecular weight (natural polymers), such as proteins (collagen, silk, and keratin), carbohydrates (starch and glycogen), lignin, cellulose, high molecular weight phenolics (tannins and derivatives), and low molecular weight active substances, such as cold-pressed oils, essential oils (organic volatile compounds, generally of low molecular weight,

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Chitosan hydrogel formation using laccase activated phenolics as cross-linkers

Cited by 83 publications

References 63 publications

The chemo enzymatic functionalization of chitosan zeolite particles provides antioxidant and antimicrobial properties

The chemo enzymatic functionalization of chitosan zeolite particles provides antioxidant and antimicrobial properties

Applications of Microbial Laccases: Patent Review of the Past Decade (2009–2019)

Vegetable Additives in Food Packaging Polymeric Materials

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