Chitosan-heparin nanoparticle coating on anodized NiTi for improvement of blood compatibility and biocompatibility

Mohammadi, Fatemeh; Golafshan, Nasim; Kharaziha, Mahshid; Ashrafi, Ali

doi:10.1016/j.ijbiomac.2019.01.026

Cited by 50 publications

(25 citation statements)

References 57 publications

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“…These tests were done according to the reported literature. 40,42,43 Hemolysis is the breakdown of red blood cells that carry O 2 to the cells to release hemoglobin into the medium. To assume a material is blood compatible, the hemolysis% range should be at least 0%-5%.…”

Section: Blood Compatibility Results For Bare and Modified P(mal) Pmentioning

confidence: 99%

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Versatile poly(maltose) micro/nanoparticles with tunable surface functionality as a biomaterial

Kurt

Ayyala

Şahiner

2020

J of Applied Polymer Sci

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Maltose, a natural disaccharide, was crosslinked with divinyl sulfone to prepare poly(maltose) (p(MAL)) micro/nanoparticles via one step microemulsion system with ≥90% ± 5% yield in a size rage of 0.5-100 μm for the first time. P(MAL) was modified (m-p(MAL)) with ethylenediamine (EDA), polyethyleneimine (PEI), and taurine (TA) to render additional functionalities, that is, amine and sulfate groups. The isoelectronic point of bare p(MAL) particles were calculated at pH 2.2 ± 0.5 and was changed to 1.3 ± 0.5, 4.3 ± 1.0, and 8.1 ± 0.7 for TA (p(MAL)/TA), EDA (p(MAL)/EDA), and PEI (p(MAL)/PEI) modification, respectively. Bare p(MAL) particles were found to be biocompatible up to 2 mg/ml with hemolysis and blood clotting tests, whereas the modified p(MAL) particles were found to be biocompatible at 1 mg/ml concentration. Additionally, it was found that TA-and PEI-modified p(MAL) particles induced blood clotting mechanisms. Sodium diclofenac as model drug was released at proportions of 8.7% ± 1.3%, 3.9% ± 0.2%, 8.8% ± 0.9%, and 31.6% ± 0.4% of the loaded drug in phosphate buffered saline solution from p(MAL), p(MAL)/TA, p(MAL)/EDA, and p(MAL)/PEI, respectively. The inhibition of antimicrobial activity of p(MAL)/PEI particles at 20 mg/ml concentration for Escherichia coli and Staphylococcus aureus strain was determined as 99.86% ± 0.3% and 99.79% ± 0.25%, respectively.

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Section: Blood Compatibility Results For Bare and Modified P(mal) Pmentioning

confidence: 99%

“…The blood compatibility assays are significantly pertinent for biomedicinal use of p(MAL) particles that may be used as blood contacting material. These tests were done according to the reported literature 40,42,43 . Hemolysis is the breakdown of red blood cells that carry O 2 to the cells to release hemoglobin into the medium.…”

Section: Resultsmentioning

confidence: 99%

Versatile poly(maltose) micro/nanoparticles with tunable surface functionality as a biomaterial

Kurt

Ayyala

Şahiner

2020

J of Applied Polymer Sci

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“…Blood compatible materials should not interact with protein and blood cells to be used in vivo or ex vivo such as wound dressing and open scar application. Hemolysis and blood clotting tests are generally applied in accord to the published procedure 42,43 . Hemolysis is bursting of red blood cells and hemoglobin is released from these cells making the determination of hemolysis percent measurable with UV‐Vis spectrum.…”

Section: Resultsmentioning

confidence: 99%

Chitosan based fibers embedding carbon dots with anti‐bacterial and fluorescent properties

Kurt

Şahiner

2020

Polymer Composites

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Due to the intriguing properties of Chitosan (CS), many efforts have been paid for CS‐based fiber preparation. As it is impossible to prepare CS fiber directly, facilitating polymers such as polyvinyl alcohol (PVA) possessing necessary properties that enable CS‐based fiber fabrication is used. For this purpose, 3 wt% of CS in 90% acetic acid solution and 6 wt% PVA solution in DI water used in CS/PVA fiber preparation at different proportions for example, 2:1, 9:1 and 10:1 by weight to fabricate fibers by electrospinning method. Furthermore, to render fluorescent property to CS/PVA (9:1) fibers, N‐doped carbon dots (C‐dots) added into fiber precursor during fiber preparation. The emission peak of C‐dots based fibers that with fluorescent features was observed at around 436 nm. The morphological, structural and, thermal characterizations of CS‐based fibers were done with scanning electron microscopy, Fourier transform infrared spectroscopy, and thermogravimetric analyzer, respectively. Anti‐bacterial activity of bare CS/PVA was increased with the increase in amounts of C‐dots embedding. The inhibition of zone of CS/PVA (9:1) fibers was increased from 1.8 ± 0.2 cm to 2.1 ± 0.3 cm and 2.9 ± 0.4 cm against E. coli by increasing the amounts of C‐dots from 2.9 mg and 5 mg. Also, the same behavior was observed against S. aureus where the inhibition zone is increased from 2.5 ± 0.1 cm to 3.2 and 3.5 cm by doubling the C‐dots amounts in CS/PVA fiber. Moreover, bare and C‐dots containing CS/PVA fibers were found blood compatible (nonhemolytic) up to 1 mg/mL concentration according to hemolysis and blood clotting tests.

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“…CS utilization in drugs is extremely varied. However, tissue engineering (TE) and wound dressing (WD) tend to be its two primary applications [ 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 ]. It was exhibited [ 68 ] that CS might be employed to hinder fibroplasia in wound healing and to enhance cell growth and attachment.…”

Section: Chitosan (Cs)mentioning

confidence: 99%

Three-Dimensional Printing Constructs Based on the Chitosan for Tissue Regeneration: State of the Art, Developing Directions and Prospect Trends

et al. 2020

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Chitosan (CS) has gained particular attention in biomedical applications due to its biocompatibility, antibacterial feature, and biodegradability. Hence, many studies have focused on the manufacturing of CS films, scaffolds, particulate, and inks via different production methods. Nowadays, with the possibility of the precise adjustment of porosity size and shape, fiber size, suitable interconnectivity of pores, and creation of patient-specific constructs, 3D printing has overcome the limitations of many traditional manufacturing methods. Therefore, the fabrication of 3D printed CS scaffolds can lead to promising advances in tissue engineering and regenerative medicine. A review of additive manufacturing types, CS-based printed constructs, their usages as biomaterials, advantages, and drawbacks can open doors to optimize CS-based constructions for biomedical applications. The latest technological issues and upcoming capabilities of 3D printing with CS-based biopolymers for different applications are also discussed. This review article will act as a roadmap aiming to investigate chitosan as a new feedstock concerning various 3D printing approaches which may be employed in biomedical fields. In fact, the combination of 3D printing and CS-based biopolymers is extremely appealing particularly with regard to certain clinical purposes. Complications of 3D printing coupled with the challenges associated with materials should be recognized to help make this method feasible for wider clinical requirements. This strategy is currently gaining substantial attention in terms of several industrial biomedical products. In this review, the key 3D printing approaches along with revealing historical background are initially presented, and ultimately, the applications of different 3D printing techniques for fabricating chitosan constructs will be discussed. The recognition of essential complications and technical problems related to numerous 3D printing techniques and CS-based biopolymer choices according to clinical requirements is crucial. A comprehensive investigation will be required to encounter those challenges and to completely understand the possibilities of 3D printing in the foreseeable future.

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Chitosan-heparin nanoparticle coating on anodized NiTi for improvement of blood compatibility and biocompatibility

Cited by 50 publications

References 57 publications

Versatile poly(maltose) micro/nanoparticles with tunable surface functionality as a biomaterial

Versatile poly(maltose) micro/nanoparticles with tunable surface functionality as a biomaterial

Chitosan based fibers embedding carbon dots with anti‐bacterial and fluorescent properties

Three-Dimensional Printing Constructs Based on the Chitosan for Tissue Regeneration: State of the Art, Developing Directions and Prospect Trends

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