Chitosan–glutathione conjugate-coated poly(butyl cyanoacrylate) nanoparticles: Promising carriers for oral thymopentin delivery

Jin, Xuefeng; Xu, Ying; Shen, Jie; Ping, Qineng; Su, Zhigui; You, Weiliang

doi:10.1016/j.carbpol.2011.03.050

Cited by 31 publications

(15 citation statements)

References 37 publications

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“…28 CTS-GS (0.5 g) was dissolved in 35 mL of distilled water. Then 5 mL of EDC, NHS and GSH solutions were separately added to the CTS-GS solution at a molar ratio of 5:5:3:2.…”

Section: Synthesis and Characterization Of Cg-gs Synthesis Of Cg-gsmentioning

confidence: 99%

Functional intercalated nanocomposites with chitosan-glutathione-glycylsarcosine and layered double hydroxides for topical ocular drug delivery

Xu¹,

Xu²,

Gu³

et al. 2018

IJN

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Background To enhance ocular bioavailability, the traditional strategies have focused on prolonging precorneal retention and improving corneal permeability by nano-carriers with positive charge, thiolated polymer, absorption enhancer and so on. Glycylsarcosine (GS) as an active target ligand of the peptide tranpsporter-1 (PepT-1), could specific interact with the PepT-1 on the cornea and guide the nanoparticles to the treating site. Purpose The objective of the study was to explore the active targeting intercalated nanocomposites based on chitosan-glutathione-glycylsarcosine (CG-GS) and layered double hydroxides (LDH) as novel carriers for the treatment of mid-posterior diseases. Materials and methods CG-GS-LDH intercalated nanocomposites were prepared by the coprecipitation hydrothermal method. In vivo precorneal retention study, ex vivo fluorescence images, in vivo experiment for distribution and irritation were studied in rabbits. The cytotoxicity and cellular uptake were studied in human corneal epithelial primary cells (HCEpiC). Results CG-GS-LDH nanocomposites were prepared successfully and characterized by FTIR and XRD. Experiments with rabbits showed longer precorneal retention and higher distribution of fluorescence probe/model drug. In vitro cytological study, CG-GS-LDH nanocomposites exhibited enhanced cellular uptake compared to pure drug solution. Furthermore, the investigation of cellular uptake mechanisms demonstrated that both the active transport by PepT-1 and clathrin-mediated endocytosis were involved in the internalization of CG-GS-LDH intercalated nanocomposites. An ocular irritation study and a cytotoxicity test indicated that these nanocomposites produced no significant irritant effects. Conclusions The active targeting intercalated nanocomposites could have great potential for topical ocular drug delivery due to the capacity for prolonging the retention on the ocular surface, enhancing the drug permeability through the cornea, and efficiently delivering the drug to the targeted site.

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“…28 CTS-GS (0.5 g) was dissolved in 35 mL of distilled water. Then 5 mL of EDC, NHS and GSH solutions were separately added to the CTS-GS solution at a molar ratio of 5:5:3:2.…”

Section: Synthesis and Characterization Of Cg-gs Synthesis Of Cg-gsmentioning

confidence: 99%

Functional intercalated nanocomposites with chitosan-glutathione-glycylsarcosine and layered double hydroxides for topical ocular drug delivery

Xu¹,

Xu²,

Gu³

et al. 2018

IJN

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“…The mixture was incubated at room temperature for 45 min. The unreacted EDAC was removed by centrifugation and the nanoparticles were resuspended in 2.4 mL phosphate buffer (pH 7.4) (Nobs et al, 2003;Jin et al, 2011). The same volume of glutathione solution (50 mg/mL) was added to the dispersion containing the activated nanoparticles and the mixture was stirred gently at room temperature for 6 h. The conjugated nanoparticles were centrifuged for 30 min at 15 000 g (Biolab, Mumbai, India), to remove excess of glutathione.…”

Section: Glutathione Conjugation To Bsa Nanoparticlesmentioning

confidence: 99%

Development and characterization of glutathione-conjugated albumin nanoparticles for improved brain delivery of hydrophilic fluorescent marker

Patel

Acharya

2013

Drug Delivery

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The glutathione-conjugated bovine serum albumin (BSA) nanoparticles were constructed in the present exploration as a novel biodegradable carrier for brain-specific drug delivery with evaluation of its in vitro and in vivo delivery properties. BSA nanocarriers were activated and conjugated to the distal amine functions of the glutathione via carbodiimide chemistry using EDAC as a mediator. These nanoparticles were characterized for particle shape, average size, SPAN value, drug entrapment and in vitro drug release. Further, presence of glutathione on the surface of BSA nanoparticles was confirmed by Ellman's assay, which has suggested that approximately 750 units of glutathione were conjugated per BSA nanoparticle. To evaluate the brain delivery properties of the glutathione-conjugated BSA nanoparticles fluorescein sodium was used as a model hydrophilic compound. Permeability and neuronal uptake properties of developed formulations were evaluated against the MDCK-MDR1 endothelial and neuro-glial cells, respectively. The permeability of glutathione-conjugated BSA nanoparticles across the monolayer of MDCK-MDR1 endothelial tight junction was shown significantly higher than that of unconjugated nanoparticles and fluorescein sodium solution. Similarly, glutathioneconjugated nanoparticles exhibited considerably higher uptake by neuro-glial cells which was inferred by high fluorescence intensity under microscope in comparison to unconjugated nanoparticles and fluorescein sodium solution. Following an intravenous administration, nearly three folds higher fluorescein sodium was carried to the rat brain by glutathione-conjugated nanoparticles as compared to unconjugated nanoparticles. The significant in vitro and in vivo results suggest that glutathione-conjugated BSA nanoparticles is a promising brain drug delivery system with low toxicity.

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“…Few other synthetic polymers have been suggested but their use remains marginal so far [Amoozgar and Yeo, 2012]. In contrast, there is a growing interest using polysaccharides as an alternative to PEG [Lemarchand et al, 2004, Jin et al, 2011, Yu et al, 2014, Ibegbu et al, 2017, Juenet et al, 2018. A method for the obtaining of stealth PACA nanoparticles using polysaccharides as coating materials was developed from a radical emulsion polymerization of alkylcyanoacrylate monomers [Chauvierre et al, 2003, Alhareth et al, 2012.…”

Section: Designing Nanoparticles Controlling Their In Vivo Drug Delivmentioning

confidence: 99%

A journey through the emergence of nanomedicines with poly(alkylcyanoacrylate) based nanoparticles

Vauthier

2019

Journal of Drug Targeting

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Starting in the late 1970s, the pioneering work of Patrick Couvreur gave birth to the first biodegradable nanoparticles composed of a biodegradable synthetic polymer. These nanoparticles, made of poly(alkylcyanoacrylate) (PACA), were the first synthetic polymer based nanoparticulate drug carriers undergoing a phase III clinical trial so far. Analyzing the journey from the birth of PACA nanoparticles to their clinical evaluation, the paper highlights their remarkable adaptability to bypass various drug delivery challenges found on the way. At present, PACA nanoparticles includes a wide range of nanoparticles that can associate drugs of different chemical nature and be administered in vivo by different routes. The most recent technologies giving the nanoparticles customized functions could also be implemented on this family of nanoparticles. Through different examples, the paper discusses the seminal role of the PACA nanoparticles' family in the development of nanomedicines.

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Chitosan–glutathione conjugate-coated poly(butyl cyanoacrylate) nanoparticles: Promising carriers for oral thymopentin delivery

Cited by 31 publications

References 37 publications

Functional intercalated nanocomposites with chitosan-glutathione-glycylsarcosine and layered double hydroxides for topical ocular drug delivery

Functional intercalated nanocomposites with chitosan-glutathione-glycylsarcosine and layered double hydroxides for topical ocular drug delivery

Development and characterization of glutathione-conjugated albumin nanoparticles for improved brain delivery of hydrophilic fluorescent marker

A journey through the emergence of nanomedicines with poly(alkylcyanoacrylate) based nanoparticles

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