Chitosan films for regenerative medicine: fabrication methods and mechanical characterization of nanostructured chitosan films

Masi, Alessia De; Tonazzini, Ilaria; Masciullo, Cecilia; Mezzena, Roberta; Chiellini, Emo; Puppi, Dario; Cecchini, Marco

doi:10.1007/s12551-019-00591-6

Cited by 40 publications

(24 citation statements)

References 79 publications

(71 reference statements)

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“…Aqueous solubility, biocompatibility, biodegradability, antibacterial and antifungal activity, mucoadhesive and homeostatic features make chitosan as a good basis for cell attachment and growth 13 , 14 . Although, chitosan films are formed easily by the solvent casting and subsequent evaporation of the solvent 15 , the films show poor mechanical properties, and are easily broke down 16 . Poor mechanical property is the main drawback of chitosan and some other natural biomaterials which limits their application in designing the scaffold.…”

Section: Introductionmentioning

confidence: 99%

Biofabrication of chitosan/chitosan nanoparticles/polycaprolactone transparent membrane for corneal endothelial tissue engineering

Tayebi

Baradaran‐Rafii

Hajifathali

et al. 2021

Sci Rep

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We aimed to construct a biodegradable transparent scaffold for culturing corneal endothelial cells by incorporating chitosan nanoparticles (CSNPs) into chitosan/polycaprolactone (PCL) membranes. Various ratios of CSNP/PCL were prepared in the presence of constant concentration of chitosan and the films were constructed by solvent casting method. Scaffold properties including transparency, surface wettability, FTIR, and biocompatibility were examined. SEM imaging, H&E staining, and cell count were performed to investigate the HCECs adhesion. The phenotypic maintenance of the cells during culture was investigated by flow cytometry. Transparency and surface wettability improved by increasing the CSNP/PCL ratio. The CSNP/PCL 50/25, which has the lowest WCA, showed comparable transparency with human acellular corneal stroma. The scaffold was not cytotoxic and promoted the HCECs proliferation as evaluated by MTT assay. Cell counting, flow cytometry, SEM, and H&E results showed appropriate attachment of HCECs to the scaffold which formed a compact monolayer. The developed scaffold seems to be suitable for use in corneal endothelial regeneration in terms of transparency and biocompatibility.

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Section: Introductionmentioning

confidence: 99%

Biofabrication of chitosan/chitosan nanoparticles/polycaprolactone transparent membrane for corneal endothelial tissue engineering

Tayebi

Baradaran‐Rafii

Hajifathali

et al. 2021

Sci Rep

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“…Also, for conduit wall fitting nerve diameter should be taken into account and nerve elasticity changes depending on nerve size (∼21,188 Pa for a pig sciatic nerve and ∼10,910,000 Pa for a human median nerve) and on how distal the nerve lesion is from the spinal cord (Stouthandel et al, 2020). Then, it must be considered that when Young's modulus is reported in the validation studies of a biomaterial, only partial information is given referring to the NGC outer layer mechanical behavior, a characteristic which can be modified by the biomaterial dryness (De Masi et al, 2019) and by the concentration and type of fillers that generally increase the NGC stiffness (Ryan et al, 2017;Tonda-Turo et al, 2017a;Vijayavenkataraman et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

Natural-Based Biomaterials for Peripheral Nerve Injury Repair

Fornasari

Carta

Gambarotta

et al. 2020

Front. Bioeng. Biotechnol.

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Peripheral nerve injury treatment is a relevant problem because of nerve lesion high incidence and because of unsatisfactory regeneration after severe injuries, thus resulting in a reduced patient's life quality. To repair severe nerve injuries characterized by substance loss and to improve the regeneration outcome at both motor and sensory level, different strategies have been investigated. Although autograft remains the gold standard technique, a growing number of research articles concerning nerve conduit use has been reported in the last years. Nerve conduits aim to overcome autograft disadvantages, but they must satisfy some requirements to be suitable for nerve repair. A universal ideal conduit does not exist, since conduit properties have to be evaluated case by case; nevertheless, because of their high biocompatibility and biodegradability, natural-based biomaterials have great potentiality to be used to produce nerve guides. Although they share many characteristics with synthetic biomaterials, natural-based biomaterials should also be preferable because of their extraction sources; indeed, these biomaterials are obtained from different renewable sources or food waste, thus reducing environmental impact and enhancing sustainability in comparison to synthetic ones. This review reports the strengths and weaknesses of natural-based biomaterials used for manufacturing peripheral nerve conduits, analyzing the interactions between natural-based biomaterials and biological environment. Particular attention was paid to the description of the preclinical outcome of nerve regeneration in injury repaired with the different natural-based conduits.

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“…The 13 contributed Letters and Reviews address fundamental basic science questions such as the reasons for heterogeneity in cell populations (Vishwakarma and Di Russo 2019) and mechanotransduction principles in neurons and other cell types (Chighizola et al 2019;Ridone et al 2019). The review articles also present latest technological developments relating to high speed atomic force microscopy (Valotteau et al 2019) and optical trapping and microscopy techniques (Boeri et al 2019;Arbore et al 2019) along with relevant nanofabrication approaches (De Masi et al 2019).…”

Section: -A Year In Reviewmentioning

confidence: 99%