Chitosan Extraction from Goliathus orientalis Moser, 1909: Characterization and Comparison with Commercially Available Chitosan

Abstract

Chitosan is a polymer obtained by deacetylation of chitin, and chitin is one of the major components of the arthropod cuticle. Chitin and chitosan are both polysaccharides and are considered to be an interesting class of biosourced materials. This is evident as chitosan has already demonstrated utility in various applications in both industrial and biomedical domains. In the present work, we study the possibility to extract chitin and prepare chitosan from the Goliath beetle Goliathus orientalis Moser. The presented work includes description of this process and observation of the macroscopic and microscopic variations that occur in the specimen during the treatment. The prepared chitosan is characterized and compared with commercially available chitosan using infrared and thermogravimetric analysis. The deacetylation degree of prepared chitosan is also evaluated and compared with commercially available shrimp chitosan.

Investigation on dung beetle's (Heliocopris Hope, 1838) chitosan valorisation for hydrogel 3D printing

“…After demineralisation, the surface presents similar morphology without any specific structure. This lack of modification after demineralisation is not surprising and is consistent with our previous work on chitin extraction 40,41. After deproteination and deacetylation, the surface presents significantly different morphologies (Figures9 C and D) and revealing slight roughness.…”

“…Our first work in this area investigated the potential of large beetles (specifically, Mecynorhina torquata Drury, 1782 and Goliathus orientalis Moser, 1909) as a source of chitosan. 40,41 To diversify our range of studied specimens, we then extended our research field from giant beetles (Cetoninae) to other beetles, including Scarabaeinae subfamily and more precisely on the dung beetle from genus Heliocopris Hope, 1838. While dung beetle breeding conditions may be considered a severe drawback for industrial production, the motivation here is to take animals that develop and live near dejection and convert them into a material appropriate for biomedical applications.…”

Nutritional Qualities and Enhancement of Edible Insects

“…The growth of insect farming, sustainability concerns, and interest in insects as human food and animal feed have led to an interest in using insect exoskeletons as a potential source of chitosan (47). The characteristics and quality of the chitosan extracted from the Goliath beetle Goliathus orientalis were similar to the chitosan extracted from shrimp (41). If functional properties are similar, insects may address the gap in chitosan supply chains caused by seasonal collection of crustaceans and provide a more sustainable alternative to fishing.…”

A biopolymer with antimicrobial properties and plant resistance inducer against phytopathogens: Chitosan

“…Notulae Botanicae Horti Cluj-Napoca Agrobotanici 2 Chitosan is extracted from chitin found in the exoskeleton of crustaceans (Fournier et al, 2020), fungal cell wall (Chang et al, 2019), insect cuticle (Luo et al, 2019), among others. Chitosan has been used in waste water treatment (Uragami et al, 2015), cosmetology (Aranaz et al, 2018), medicine (Ahsan et al, 2018), pharmaceutics (Khan et al, 2019), food and beverage (Rocha et al, 2017), and paper industries (Song et al, 2018), among others.…”

“…It is a lineal polymer formed by N-D glucosamine (2-amino-2-deoxy-β-D glucopyranose) monomers, bound by β-1-4 (Katiyar et al, 2015). Chitin is a natural and abundant biopolymer found in many organism (Fournier et al, 2020;López-Corona et al, 2020) (Table 1) and is compound of 2-acetylamine-2-deoxy-β-Dglucopyranose units (Peter et al, 2020). The main forms of application of chitosan are: seed coating, soil enrichment, foliar spraying, fruit coating, nanoparticles, among others (Morin-Crini et al, 2019).…”