Chitosan extracted from Aspergillus flavus shows synergistic effect, eases quorum sensing mediated virulence factors and biofilm against nosocomial pathogen Pseudomonas aeruginosa

Muslim, Sahira Nsayef; Al-Kadmy, Israa M.S.; Ali, Alaa Naseer Mohammed; Salman, Batool Kadham; Ahmad, Mohammad; Khazaal, Saba Saadoon; Hussein, Nadheema Hammood; Muslim, Sraa Nsayef

doi:10.1016/j.ijbiomac.2017.08.146

Cited by 40 publications

(32 citation statements)

References 12 publications

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“…Thus, it is tempting to speculate that the Ch-derivatives tested herein could somehow interfere with QS signals that promote biofilm formation and expression of virulence factors. This hypothesis is supported by recent reports that describe ability of extracted Ch to down regulate the expression of QS regulated genes in P. aeruginosa [40,41].…”

Section: Prevention Of Biofilm Formationsupporting

confidence: 80%

Antibacterial, Antibiofilm, and Antiadhesive Properties of Different Quaternized Chitosan Derivatives

Piras

Esin

Benedetti

et al. 2019

IJMS

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In the era of antimicrobial resistance, the identification of new antimicrobials is a research priority at the global level. In this regard, the attention towards functional antimicrobial polymers, with biomedical/pharmaceutical grade, and exerting anti-infective properties has recently grown. The aim of this study was to evaluate the antibacterial, antibiofilm, and antiadhesive properties of a number of quaternized chitosan derivatives that have displayed significant muco-adhesive properties and wound healing promotion features in previous studies. Low (QAL) and high (QAH) molecular weight quaternized chitosan derivatives were synthetized and further modified with thiol moieties or pendant cyclodextrin, and their antibacterial activity evaluated as minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC). The ability of the derivatives to prevent biofilm formation was assessed by crystal violet staining. Both QAL and QAH derivatives exerted a bactericidal and/or inhibitory activity on the growth of P. aeruginosa and S. epidermidis. The same compounds also showed marked dose-dependent anti-biofilm activity. Furthermore, the high molecular weight derivative (QAH) was used to functionalize titanium plates. The successful functionalization, demonstrated by electron microscopy, was able to partially inhibit the adhesion of S. epidermidis at 6 h of incubation. The shown ability of the chitosan derivatives tested to both inhibit bacterial growth and/or biofilm formation of clinically relevant bacterial species reveals their potential as multifunctional molecules against bacterial infections.

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Section: Prevention Of Biofilm Formationsupporting

confidence: 80%

Antibacterial, Antibiofilm, and Antiadhesive Properties of Different Quaternized Chitosan Derivatives

Piras

Esin

Benedetti

et al. 2019

IJMS

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“…Recently, chitosan was reported having antibacterial and anti-quorum sensing activity whereas sodium alginate did not. The QS dependent phenotype virulence factors and biofilms was significantly reduced in the influence of chitosan (Muslim et al 2017). Interestingly, it seemed that chtiosan showed no inhibitions when it diffused into sodium alginate network and formed independent structure by polyelectrolyte reaction.…”

Section: Resultsmentioning

confidence: 99%

Improving 3-phenyllactic acid production of Lactobacillus plantarum AB-1 by enhancing its quorum-sensing capacity

Yang

Shi

et al. 2019

J Food Sci Technol

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3-Phenyllactic acid (PLA) is a novel and natural antimicrobial compound. However, the concentration of PLA produced by native microbes was rather low. To enhance the production of PLA of Lactobacillus plantarum AB-1, the microcapsules of L. plantarum AB-1 cells with a high quorum-sensing capacity was established and investigated. In addition, the relation between PLA production and quorum sensing was further investigated and confirmed by adding the exogenous 4,5-dihydroxy-2,3-pentanedione (DPD, AI-2 precursor). The results indicated that the PLA production of L. plantarum AB-1 in microencapsulated cells (MC cells) was higher than that of the free cells, and the lactate dehydrogenase activity, autoinducer-2 (AI-2) levels and the relative expression of the luxS gene were also significantly increased in MC cells (P \ 0.05). In addition, the cell growth, AI-2 levels and PLA production of L. plantarum AB-1 were also significantly promoted after adding 24 lM exogenous DPD. The results suggest that the PLA production of L. plantarum was partly regulated by the AI-2/LuxS system, and microencapsulation can increase the local AI-2 level and enhance QS capacity, which are beneficial to PLA production. The results may provide a new insight and experimental basis for the industrial production of PLA.

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“…33,[97][98][99] Furthermore, chitosan NPs have shown anti-QS properties by disrupting biofilm production, decreasing the expression of lasR and rhlR genes, and reducing the secretion of pyocyanin and proteases in P. aeruginosa. 100 In addition, chitosan NPs have a negative effect on the pathogenic factors produced by P. aeruginosa PAO1. 101 In this regard, Muslim et al, 100 in their study demonstrated that chitosan significantly decrease the biofilm formation, pyocyanin, protease secretion, as well as the expressions of lasR and rhlR genes in P. aeruginosa.…”

Section: Synthetic Compoundsmentioning

confidence: 99%

<p>Quorum Quenching: A Potential Target for Antipseudomonal Therapy</p>

Hemmati¹,

Salehi²,

Ghotaslou³

et al. 2020

IDR

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There has been excessive rate of use of antibiotics to fight Pseudomonas aeruginosa (P. aeruginosa) infections worldwide, which has consequently caused the increased resistance to multiple antibiotics in this pathogen. Due to the widespread resistance and the current poor effect of antibiotics consumed to treat P. aeruginosa infections, finding some novel alternative therapeutic methods are necessary for the treatment of infections. The P. aeruginosa biofilms can cause severe infections leading to the increased antibiotic resistance and mortality rate among the patients. In this regard, there are no approaches that can efficiently manage these infections; therefore, novel and effective antimicrobial and antibiofilm agents are needed to control and treat these bacterial infections. Quorum sensing inhibitors (QSIs) or quorum quenchings (QQs) are now considered as potential therapeutic alternatives and/or adjuvants to the current failing antibiotics, which can control the virulence traits of the pathogens, so as a result, the host immune system can quickly eliminate bacteria. Thus, the aims of this review article were presenting a brief explanation of the research reports on the natural and synthetic QSIs of P. aeruginosa, and the assessment of the current understanding on the QS mechanisms and various QQ strategies in P. aeruginosa.

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Chitosan extracted from Aspergillus flavus shows synergistic effect, eases quorum sensing mediated virulence factors and biofilm against nosocomial pathogen Pseudomonas aeruginosa

Cited by 40 publications

References 12 publications

Antibacterial, Antibiofilm, and Antiadhesive Properties of Different Quaternized Chitosan Derivatives

Antibacterial, Antibiofilm, and Antiadhesive Properties of Different Quaternized Chitosan Derivatives

Improving 3-phenyllactic acid production of Lactobacillus plantarum AB-1 by enhancing its quorum-sensing capacity

<p>Quorum Quenching: A Potential Target for Antipseudomonal Therapy</p>

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