Chitosan-DNA polyelectrolyte complex: Influence of chitosan characteristics and mechanism of complex formation

Bravo-Anaya, Lourdes Mónica; Fernández-Solís, Karla Gricelda; Rosselgong, Julien; Nano-Rodríguez, Jesrael Luz Elena; Carvajal, Francisco; Rinaudo, Marguerite

doi:10.1016/j.ijbiomac.2019.01.008

Cited by 54 publications

(44 citation statements)

References 59 publications

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“…Third, single-stranded RNA is fragile and easily degraded by RNAses that are widely present in the environment, thus the process of RNA extraction often needs to be performed in a clean bench. Chitosan molecules condense efficiently with nucleic acids forming polyplexes and preventing its degradation by RNAses [32]. In this way, our device could deploy in some resource-limited area.…”

Section: Nucleic Acids Extraction Strategy On the Capillarymentioning

confidence: 99%

A Novel Microfluidic Device Integrated with Chitosan-Modified Capillaries for Rapid ZIKV Detection

Zhu

Zhao

et al. 2020

Micromachines

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The outbreak of Zika virus (ZIKV) has posed a great challenge to public health in recent years. To address the urgent need of ZIKV RNA assays, we integrate the microfluidic chip embedded with chitosan-modified silicon dioxide capillaries, smartphone-based detection unit to be a C3-system for the rapid extraction and detection of ZIKV RNA. The C3-system is characterized by: (1) four chitosan-modified silicon dioxide capillaries integrated in the microfluidic chip for target ZIKV RNA enrichment and “in situ PCR” (polymerase chain reaction) amplification; (2) smartphone-based point of care (POC) device consisting of a pneumatic subsystem for controlling the nucleic acid extraction processes in the microfluidic chip, a heating subsystem for sample lysis and PCR amplification, and an optical subsystem for signal acquisition. The entire detection processes including sample lysis, ZIKV RNA enrichment, and reverse-transcription polymerase chain reaction (RT-PCR) is achieved in the microfluidic chip. Moreover, PCR buffers can be directly loaded into the chitosan-modified silicon dioxide capillaries for “in situ PCR”, in which the captured ZIKV RNA is directly used for downstream PCR without any loss. ZIKV RNA extracted by the C3-system can be successfully recovered at very low concentrations of 50 transducing units (TU)/mL from crude human saliva. This means that our method of detecting viremia in patients infected with ZIKV is reliable.

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Section: Nucleic Acids Extraction Strategy On the Capillarymentioning

confidence: 99%

A Novel Microfluidic Device Integrated with Chitosan-Modified Capillaries for Rapid ZIKV Detection

Zhu

Zhao

et al. 2020

Micromachines

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“…Slow dropwise mixing of cationic hybrid ELPs to a negatively charged DNA solution was selected as complex formation method. 71,[76][77][78] The stabilization of pDNA/Cationic hybrid ELPs complexes was reached after 5 min. of each cationic ELP addition.…”

Section: Size Exclusion Chromatography (Sec) Analysesmentioning

confidence: 99%

“…71 H NMR measurements taking the theoretical molar mass of the initial ELP (17,035 g/mol). 24 The % of functionalization was multiplied by 11 available methionine residues and by the molar mass of the respective cationic oligomer grafted for each coupling (P(TMAEMA) 6 , P(TMAEMA) 9 66,78,90,94 have higher charge densities and tend to destabilize cell membrane at high N + /P charge ratios. It is well known that this charge ratio strongly influences the nucleic acid nanocarrier's ζ-potential.…”

Section: Fig 5 Size Exclusion Chromatography Traces Inmentioning

confidence: 99%

Coupling of RAFT polymerization and chemoselective post-modifications of elastin-like polypeptides for the synthesis of gene delivery hybrid vectors

et al. 2021

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“…Natural polymers such as chitosan and hyaluronic acid, are highly biocompatible particles [208,209]. As these particles present a high positive charge that allows efficient RNA loading [210], they present, in contrast, difficulty to release the RNA oligos from the complex.…”

Section: Polymeric Particlesmentioning

confidence: 99%

The Promise and Challenges of Developing miRNA-Based Therapeutics for Parkinson’s Disease

Titze‐de‐Almeida

Soto-Sánchez

Fernández

et al. 2020

Cells

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MicroRNAs (miRNAs) are small double-stranded RNAs that exert a fine-tuning sequence-specific regulation of cell transcriptome. While one unique miRNA regulates hundreds of mRNAs, each mRNA molecule is commonly regulated by various miRNAs that bind to complementary sequences at 3’-untranslated regions for triggering the mechanism of RNA interference. Unfortunately, dysregulated miRNAs play critical roles in many disorders, including Parkinson’s disease (PD), the second most prevalent neurodegenerative disease in the world. Treatment of this slowly, progressive, and yet incurable pathology challenges neurologists. In addition to L-DOPA that restores dopaminergic transmission and ameliorate motor signs (i.e., bradykinesia, rigidity, tremors), patients commonly receive medication for mood disorders and autonomic dysfunctions. However, the effectiveness of L-DOPA declines over time, and the L-DOPA-induced dyskinesias commonly appear and become highly disabling. The discovery of more effective therapies capable of slowing disease progression –a neuroprotective agent–remains a critical need in PD. The present review focus on miRNAs as promising drug targets for PD, examining their role in underlying mechanisms of the disease, the strategies for controlling aberrant expressions, and, finally, the current technologies for translating these small molecules from bench to clinics.

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Chitosan-DNA polyelectrolyte complex: Influence of chitosan characteristics and mechanism of complex formation

Cited by 54 publications

References 59 publications

A Novel Microfluidic Device Integrated with Chitosan-Modified Capillaries for Rapid ZIKV Detection

A Novel Microfluidic Device Integrated with Chitosan-Modified Capillaries for Rapid ZIKV Detection

Coupling of RAFT polymerization and chemoselective post-modifications of elastin-like polypeptides for the synthesis of gene delivery hybrid vectors

The Promise and Challenges of Developing miRNA-Based Therapeutics for Parkinson’s Disease

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