Chitosan-DNA Particles for DNA Delivery: Effect of Chitosan Molecular Weight on Formation and Release Characteristics

Morán, Carmen; Laranjeira, Tânia; Ribeiro, Antônio J.; Miguel, Maria G.; Lindman, Björn

doi:10.1080/01932690903123403

Cited by 10 publications

(14 citation statements)

References 22 publications

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“…The formation of the chitosan-DNA gel particles was studied using mixtures of DNA and chitosan of different molecular weight ((Low MW chitosans of ca. 50 kDa and 150 kDa, and medium MW chitosan (400 kDa) [74] .The molecular weight of the polysaccharide structure did not have a clear effect on the LC values.…”

mentioning

confidence: 94%

“…Novel chitosan-DNA gel particles have been prepared based on associative phase separation and interfacial diffusion using mixtures of DNA and chitosan of different molecular weight [74][75]. In particular it was found that the chitosan molecular weight is a good controlling parameter in the final properties of these DNA gel particles [74].…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

“…In the first 24 hours, 57%, 71%, and 74% of DNA were released from the particles containing low MW chitosans of ca. 50 kDa and 150 kDa, and medium MW chitosan (400 kDa), respectively [74].…”

mentioning

confidence: 99%

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DNA gel particles: An overview

Morán

Vinardell

Infante

et al. 2014

Advances in Colloid and Interface Science

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A general understanding of interactions between DNA and oppositely charged compounds forms the basis for developing novel DNA-based materials, including gel particles. The association strength, which is altered by varying the chemical structure of the cationic cosolute, determines the spatial homogeneity of the gelation process, creating DNA reservoir devices and DNA matrix devices that can be designed to release either single-(ssDNA) or double-stranded (dsDNA). This review covers recent developments on the topic of DNA gel particles formed in water-water emulsion-type interfaces. The degree of DNA entrapment, particle morphology, swelling/dissolution behaviour and DNA release responses are discussed as a function of the nature of the cationic agent used. On the basis of designing DNA gel particles for therapeutic purposes, recent studies on the determination of the surface hydrophobicity, the haemolytic and the cytotoxic assessments of the obtained DNA gel particles have been also reported.

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confidence: 94%

Section: Accepted M Manuscriptmentioning

confidence: 99%

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DNA gel particles: An overview

Morán

Vinardell

Infante

et al. 2014

Advances in Colloid and Interface Science

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“…This DNA concentration was chosen because it produces high-viscosity solutions, which makes it an appropriate system for the preparation of stable DNA gel particles. [3][4][5][6][7][8][9] Particles were prepared by dropwise addition of DNA solutions to the surfactant solutions, equilibrated at 25 or 45ºC. Because of the relatively high viscosity of the DNA solution, mixing of the two solutions is not instantaneous.…”

Section: Particle Preparationmentioning

confidence: 99%

“…By mixing solutions of DNA (either single-stranded (ssDNA) or double-stranded (dsDNA)) with solutions of different cationic agents, such as surfactants, proteins and polysaccharides, the possibility of the formation of DNA gel particles without adding any kind of cross-linker or organic solvent has been confirmed. [3][4][5][6][7][8][9] The strength of association, which is tuned by varying the structure of the cationic agent, allows control of the spatial homogeneity of the gelation process, producing either a homogeneous DNA matrix or different reservoir devices. This gives rise to various applications for the controlled encapsulation and release of ssDNA and dsDNA, with clear differences in their mechanism.…”

Section: Introductionmentioning

confidence: 99%

Counter-ion effect on surfactant–DNA gel particles as controlled DNA delivery systems

et al. 2012

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The ability to entrap drugs within vehicles and subsequently release them has led to new treatments for a number of diseases. Based on an associative phase separation and interfacial diffusion approach, we developed a way to prepare DNA gel particles without adding any kind of cross-linker or organic solvent. Among the various agents studied, cationic surfactants offered particularly efficient control for encapsulation and DNA release from these DNA gel particles. The driving force for this strong association is the electrostatic interaction between the two components, as induced by the entropic increase due to the release of the respective counter-ions. However, little is known about the influence of the respective counter-ions on this surfactant-DNA interaction.Here we examined the effect of different counter-ions on the formation and properties of the DNA gel particles by mixing DNA (either single-(ssDNA) or double-stranded (dsDNA)) with the single chain surfactant dodecyltrimethylammonium (DTA). In particular, we used as counter-ions of this surfactant the hydrogen sulfate and trifluoromethane sulfonate anions and the two halides, chloride and bromide. Effects on the morphology of the particles obtained, the encapsulation of DNA and its release, as well as the haemocompatibility of these particles, are presented, using the counter-ion structure and the DNA conformation as controlling parameters. Analysis of the data indicates that the degree of counter-ion dissociation from the surfactant micelles and the polar/hydrophobic character of the counter-ion are important parameters in the final properties of the particles. The stronger interaction with amphiphiles for ssDNA than for dsDNA suggests the important role of hydrophobic interactions in DNA.

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Polyelectrolyte Complexes

Dias¹,

Stokke²

2014

Polyelectrolytes

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Chitosan-DNA Particles for DNA Delivery: Effect of Chitosan Molecular Weight on Formation and Release Characteristics

Cited by 10 publications

References 22 publications

DNA gel particles: An overview

DNA gel particles: An overview

Counter-ion effect on surfactant–DNA gel particles as controlled DNA delivery systems

Polyelectrolyte Complexes

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