Chitosan-DNA nanoparticles as non-viral vectors in gene therapy: strategies to improve transfection efficacy

Mansouri, Sania; Lavigne, Patrick; Corsi, Karin A.; Benderdour, Mohamed; Beaumont, Éric; Fernandes, Julio C.

doi:10.1016/s0939-6411(03)00155-3

Cited by 486 publications

(295 citation statements)

References 57 publications

Supporting

Mentioning

288

Contrasting

Unclassified

Order By: Relevance

“…To facilitate endocytosis by cells and subsequent transfection, it is generally accepted that chitosan nanoparticles must be 100-200nm in diameter and carry an overall positive zeta potential (ZP) [15,22]. Molecular weight (MW) is one of the main parameters affecting chitosan-mediated transfection as it influences nanoparticle size, complexation efficiency between chitosan and pDNA, rate and extent of endocytosis and eventual dissociation or 'unpacking' of the pDNA from chitosan [16,[23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Development of a gene-activated scaffold platform for tissue engineering applications using chitosan-pDNA nanoparticles on collagen-based scaffolds

Raftery

Tierney

Curtin

et al. 2015

Journal of Controlled Release

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Development of a gene-activated scaffold platform for tissue engineering applications using chitosan-pDNA nanoparticles on collagen-based scaffolds

Raftery

Tierney

Curtin

et al. 2015

Journal of Controlled Release

View full text Add to dashboard Cite

“…Alternatively, non-viral synthetic vectors, though generally nonimmunogenic, show lower transfection efficiencies and significant cytotoxicity at high or repetitive doses. Among them polycationic polymers based on polyethylene imine (PEI) [3], dendrimers [4], chitosan derivatives [5,6] and polylysine [7] form nanometric complexes (polyplexes) with pDNA and siRNA have already demonstrated to enhance the uptake of the associated nucleotides [4]. But despite their ability to enhance the cellular uptake of condensed siRNA, they still exhibit low knockdown efficiency due to insufficient siRNA release [8].…”

Section: Introductionmentioning

confidence: 99%

Cellular delivery of polynucleotides by cationic cyclodextrin polyrotaxanes

Dandekar

Jain

Keil

et al. 2012

Journal of Controlled Release

View full text Add to dashboard Cite

Cationic polyrotaxanes, obtained by temperature activated threading of cationic cyclodextrin derivatives onto water soluble cationic polymers (ionenes), form metastable nanometric polyplexes with pDNA and combinations of siRNA with pDNA.Because of their low toxicity, the polyrotaxane polyplexes constitute a very interesting system for the transfection of polynucleotides into mammalian cells. The complexation of Cy3-labeled siRNA within the polyplexes was demonstrated by fluorescence correlation spectroscopy. The uptake of the polyplexes (red) was imaged by confocal fluorescence microscopy using the A549 cell line as a model (blue: nuclei, green: membranes). The results prove the potential of polyrotaxanes for further investigations involving knocking down genes of therapeutic interest.

show abstract

“…Chitosan nanoparticles have frequently used as a controlled release drug carrier for gene transfer in artificial organs and for immune prophylaxis. In addition, chitosan nanoparticles have been used to improve the strength and washability of textiles and to confer antibacterial effects (Panyam and Labhasetwar, 2003;Mansouri et al, 2004). Several researches have been demonstrated that chitosan nanoparticles are able to improve drug bioavailability, modify pharmacokinetics and protect the encapsulated drugs (Janes et al, 2001;Shi et al, 2011b).…”

Section: Chitosan Nanoparticles Applicationsmentioning

confidence: 99%

Potential Applications of Chitosan Nanoparticles as Novel Support in Enzyme Immobilization

Malmiri

Jahanian

Berenjian

2012

American Journal of Biochemistry and Biotechnology

View full text Add to dashboard Cite

Chitosan is an attractive natural biopolymer from renewable resources with the presence of reactive amino and hydroxyl functional groups in its structure. Due to the good biocompatibility of chitosan, it can be used in magnetic-field assisted drug delivery, enzyme or cell immobilization and many other industrial applications. In the past decade, nanotechnology has been a considerable research interest in the area of preparation of immobilized enzyme carriers. This study looks at characteristics and applications of chitosan and chitosan nanoparticles and their potentials as suitable supports for enzyme immobilization. Results indicated that activity of immobilized enzymes and performance of enzyme immobilization onto chitosan nanoparticles are higher than chitosan macro and microparticles. As compared to other biopolymers nanoparticles, application of chitosan nanoparticles to immobilize enzymes strongly increases stability of immobilized enzymes and their easy separability from the reaction mixture at the end of the biochemical process.

show abstract

Chitosan-DNA nanoparticles as non-viral vectors in gene therapy: strategies to improve transfection efficacy

Cited by 486 publications

References 57 publications

Development of a gene-activated scaffold platform for tissue engineering applications using chitosan-pDNA nanoparticles on collagen-based scaffolds

Development of a gene-activated scaffold platform for tissue engineering applications using chitosan-pDNA nanoparticles on collagen-based scaffolds

Cellular delivery of polynucleotides by cationic cyclodextrin polyrotaxanes

Potential Applications of Chitosan Nanoparticles as Novel Support in Enzyme Immobilization

Contact Info

Product

Resources

About