Chitosan-coated nanoliposome as vitamin E carrier

Abstract

A new nutraceutical carrier, chitosan-coated nano-size liposome, which is itself bioactive, is made from phosphatidylcholine (pc) and cholesterol by the sonication method. Different factors related to the size of the liposomes such as the ratio of the pc and cholesterol and the time of sonication are investigated. At the 40 : 60 ratios of pc and cholesterol with 12 min sonication, the smallest liposomes, with mean diameter ∼82 nm, were obtained. Incorporation of chemically labile active ingredients into the chitosan-coated liposomes prevented chemical degradation, which is shown for vitamin E (VE). After VE was loaded to the liposomes, the size of chitosan-coated liposomes increased to 144 nm. The loading efficiency and payload of VE were investigated by loading different amounts of VE to the liposomes which were prepared with different ratios of pc and cholesterol. Chitosan-coated nano-coated nano-size liposomes appeared to be promising VE carriers, with highest loading efficiency over 99% and payload over 27%. The stability of VE-loaded liposomes suspension during the 8 weeks storage is over 90% under 4°C.

Effective mucoadhesive liposomal delivery system for risedronate: preparation and in vitro/in vivo characterization

“…First, the effect of chitosan on the opening of tight-junction and absorptive endocytosis may contribute, at least in part, to enhanced intestinal absorption of risedronate from the chitosan-coated liposomes. 40,42 This is also parallel to the observations regarding uptake of Caco-2 cells. Second, as suggested by the mucin adsorption and vitro stability studies, the chitosan-coated layer might prolong drug residence time at the mucosal sites due to its mucoadhesiveness and also increase the gastrointestinal stability of the liposomal membrane, leading to enhanced membrane transport of the hydrophilic drug inside the intact liposome.…”

“…First, chitosan may facilitate the paracellular transport of drugs. 40 Chitosan can adhere to the mucosal surface, resulting in transient opening of tight junctions via translocation of the proteins ZO-1 and occludin from the plasma membrane to the cytoplasm and redistribution of F-actin. 40 As a result, chitosan may increase the paracellular transport of drugs.…”

“…40 Chitosan can adhere to the mucosal surface, resulting in transient opening of tight junctions via translocation of the proteins ZO-1 and occludin from the plasma membrane to the cytoplasm and redistribution of F-actin. 40 As a result, chitosan may increase the paracellular transport of drugs. Kudsiova and Lawrence 41 demonstrated that chitosan-coated phospholipid vesicles reduce the transepithelial electrical resistance of Caco-2 cells and increase paracellular permeability.…”

Microencapsulation of Kabocha Pumpkin Carotenoids

“…Reference [19] concluded that the loading carotenoids with high concentration did not provide better encapsulation efficiency. This phenomenon was also observed in other polymeric matrices that over loading of encapsulated materials caused some decrease in the encapsulation efficiency [20]. The centrifugation process also caused the decreasing in International Journal of Chemical Engineering and Applications, Vol.…”

Development and Evaluation of Mucoadhesive Liposomes of Repaglinide for Oral Controlled Delivery System

“…[11,12] The mucoadhesive property of the polymer- Amount of drug remaining in mucosa Mucoadhesion (%) X 100 Amount of drug taken in tests Stability studies as per ICH guidelines [13] Accelerated stability testing studies was performed for 6 months as per ICH guidelines. The optimized formulation was kept at 4 ± 2 ℃ and 75 ± 5 % RH in stability chamber.…”