Chitosan-coated electrospun nanofibers with antibacterial activity

Abstract

Charged nanofibers were prepared by electrospinning (ESP) poly(e-caprolactone) with a copolymer bearing carboxylic acid functions. The presence of these functions allowed exposing some negative charges on the fiber surface, by dipping the fibers in a phosphate buffer. A layer of chitosan, a polycation in acidic medium, was then deposited on the nanofiber surface, thanks to electrostatic attraction. Fibers were characterized at each step of the process and the influence of the copolymer architecture on chitosan deposition was discussed. The antibacterial activity of the resulting fibers was finally assessed.

Applicability of electro-osmotic flow for the analysis of the surface zeta potential

“…On the other hand several authors have reported SZP results for various material types ( [13][14][15][16][17][18][19][20][21][23][24][25][26][27][28][29][30][31][32][33][34][35] ) obtained by the EOF method (in different congurations) using mostly polymeric tracer particles. The problems observed in these studies were associated with a high measurement uncertainty that consequently lowers the quality of the data 15,16,24,27,[38][39][40] especially for more complex sample surfaces (nanobers, membranes), difficulties in determining the surface IEP when oppositely charged tracer particles were exposed, 15,18,19,26,29 or the observation of polymeric tracer particles sedimentation and degradation (colour change of tracer particle dispersion), which is even more pronounced under elevated ionic strength, 15,19,24,28 that limits the applicability of the EOF method at high salinity conditions. Moreover, results from the EOF method showed also bad reproducibility and questionable reliability.…”

Tailored PCL Scaffolds as Skin Substitutes Using Sacrificial PVP Fibers and Collagen/Chitosan Blends

“…Different authors have studied the incorporation of major biopolymers in PCL through coating or blending. It is conducted through collagen [12,13,23,26], or its denatured form, gelatin [10,27], and chitosan as a natural polysaccharide [14,28,29], or a blend of chitosan-gelatin [30]. In this work, a blend of both collagen and chitosan was coated onto the surface of electrospun PCL samples, and, to the best of the author's knowledge, this is the first study to use them as a blend for grafting on electrospun PCL mats.…”

“…After coating scaffolds with the chitosan through electrostatic interactions, they did not observe any growth of bacterial colonies in the chitosan-coated mats [14]. Ezhilarasu et al investigated loaded PCL/aloe Vera (AV) blends with curcumin (CUR) and tetracycline hydrochloride (TCH), separately, using electrospinning technique to compare their effects and reported better antibacterial activity and surface wettability for PCL/TCH in comparison with PCL/AL, which could be helpful in wound healing applications [15].…”

“…Ghosal et al prepared collagen-coated scaffolds of PCL by immersing scaffolds in collagen solutions to enhance their hydrophilicity [13]. In another study, Croisier et al produced some electrospun scaffolds of PCL and poly(MMA-MA).After coating scaffolds with the chitosan through electrostatic interactions, they did not observe any growth of bacterial colonies in the chitosan-coated mats [14]. Ezhilarasu et al investigated loaded PCL/aloe Vera (AV) blends with curcumin (CUR) and tetracycline hydrochloride (TCH), separately, using electrospinning technique to compare their effects and reported better antibacterial activity and surface wettability for PCL/TCH in comparison with PCL/AL, which could be helpful in wound healing applications [15].One of the frequently used methods for surface functionalization of polyester-based scaffolds is the aminolysis reaction, which introduces free amine groups on the surface of polymers [16].…”

Organic Nanomaterials and Their Applications in the Treatment of Oral Diseases

“…There is also a high number of research papers that have developed composite chitosan-containing nanofibers for other biomedical applications: nanofibers of chitosan/polyethylene oxide for cartilage tissue engineering [88], chitosan/polyvinyl alcohol nanofibers for oral candidiasis treatment [89,102] and poly(ε-caprolactone)-poly(ethylene glycol) copolymer nanofibrous mats that were incorporated into chitosan for the regeneration of periodontium [90]. An interesting multitude of nanocomposite materials have been fabricated for wound healing applications: chitosan/polyethylene oxide nanofibers [91,103], poly(ε-caprolactone)/chitosan nanofibers [92,104,105], chitosan/polyvinyl alcohol nanofibers [93,106,107], chitosan/collagen nanofibrous membranes [94], chitosan hydrogel/nanofibrin composite [95], chitosan/sericin-nanofibers [96], chitosan-Eudragit nanofibrous sheets [97], chitosan/arginine nanofibrous membrane [98], chitosan/gelatin/shape memory polyurethane nanofibers [99] or tannic acid/chitosan/pullulan composite nanofibers [100].…”