Chitosan-co-Hyaluronic acid porous cryogels and their application in tissue engineering

Kutlusoy, Tuğçe; Oktay, Burcu; Kayaman‐Apohan, Nilhan; Süleymanoğlu, Mediha; Kuruca, Serap Erdem

doi:10.1016/j.ijbiomac.2017.05.067

Cited by 84 publications

(39 citation statements)

References 41 publications

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“…In the gelling process by freezing‐thawing method, the solvent that is frozen at −20°C is performing as a pore producer. When the physical cross‐linking process is accomplished, the matrix that has interconnected pores is obtained when the system is thawed to room temperature . The SEM images of the gel surface in Figure demonstrate the flat surface of the plain PVA gel.…”

Section: Resultsmentioning

confidence: 99%

“…It has a high viscoelasticity, capacity for lubrication, water sorption, and water retention. Therefore, HA represents one more utilized biopolymer biomedical applications such as cartilage tissue engineering, cardiac repair, molecule delivery, valvular engineering, control of stem cell behavior, microdevices, and acellular hydrogels for regenerative therapies . Furthermore, HA has the antiadhesion property that confirmed in various reports including HA performing as a physical barrier to support the dissolution of fibrin and proliferation of mesothelial cells, preventing the inflammatory response and helping wound healing.…”

Section: Introductionmentioning

confidence: 89%

“…When the physical cross-linking process is accomplished, the matrix that has interconnected pores is obtained when the system is thawed to room temperature. 16,[31][32][33] The SEM images of the gel surface in Figure 3 demonstrate the flat surface of the plain PVA gel. With the increase of HA content in HA-PVA gel (Figure 3A, 3C, and 3E), the gel surface roughness increased, the cross-sectional SEM images of the gel reveals that the internal structure of PVA gel also changes with addition of HA ( Figure 3B, 3D, and 3F) from thick clogged up structure to porous structure, and the pore size becomes smaller with the increase of HA content in HA-PVA gel due to the more interaction between the HA and PVA chain.…”

Section: Gel Morphologymentioning

confidence: 98%

See 2 more Smart Citations

Hyaluronic acid‐poly(vinyl alcohol) composite cryo‐gel for biofunctional material application

Kuchaiyaphum

rifai

Watanabe

et al. 2018

Polymers for Advanced Techs

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Recently, applications of hyaluronic acid (HA) as a biomaterial were investigated. However, the weak structure of HA gel and the effects of using cross‐linker raised concerns during in vivo resolution. In this study, we investigated the method to solve these two problems using physical cross‐linking and compositing with poly(vinyl alcohol) (PVA). Various weight ratios of HA and PVA solutions were mixed, adjustment of pH to 1.8 using HCl then used to fabricate HA‐PVA cryo‐gel by freezing‐thawing. Young's modulus of the prepared gel rose with the increase of both HA and PVA concentrations or either one of them. We estimated that HA and PVA have exhibited these mechanical properties due to forming a double network. HA‐PVA gel showed kinetic friction force of approximately 10 times of PVA gel, while water contact angle and protein adsorption of HA‐PVA gels were remarkably decreased. The properties of the prepared gel suggest that it can be used for postoperative adhesion prevention applications.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 89%

Section: Gel Morphologymentioning

confidence: 98%

See 1 more Smart Citation

Hyaluronic acid‐poly(vinyl alcohol) composite cryo‐gel for biofunctional material application

Kuchaiyaphum

rifai

Watanabe

et al. 2018

Polymers for Advanced Techs

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“…Furthermore, HA can play an important role in angiogenesis and wound healing [12,29]. The polysaccharide is composed of d -glucuronic acid and D- N -acetyl glucosamine repeating units that can be degraded by hyaluronidase enzyme [30,31]. Hydrogel scaffolds mimicking various tissues have been made using HA [31,32].…”

Section: Introductionmentioning

confidence: 99%

Injectable Hyaluronic Acid-co-Gelatin Cryogels for Tissue-Engineering Applications

et al. 2018

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Polymeric scaffolds such as hydrogels can be engineered to restore, maintain, or improve impaired tissues and organs. However, most hydrogels require surgical implantation that can cause several complications such as infection and damage to adjacent tissues. Therefore, developing minimally invasive strategies is of critical importance for these purposes. Herein, we developed several injectable cryogels made out of hyaluronic acid and gelatin for tissue-engineering applications. The physicochemical properties of hyaluronic acid combined with the intrinsic cell-adhesion properties of gelatin can provide suitable physical support for the attachment, survival, and spreading of cells. The physical characteristics of pure gelatin cryogels, such as mechanics and injectability, were enhanced once copolymerized with hyaluronic acid. Reciprocally, the adhesion of 3T3 cells cultured in hyaluronic acid cryogels was enhanced when formulated with gelatin. Furthermore, cryogels had a minimal effect on bone marrow dendritic cell activation, suggesting their cytocompatibility. Finally, in vitro studies revealed that copolymerizing gelatin with hyaluronic acid did not significantly alter their respective intrinsic biological properties. These findings suggest that hyaluronic acid-co-gelatin cryogels combined the favorable inherent properties of each biopolymer, providing a mechanically robust, cell-responsive, macroporous, and injectable platform for tissue-engineering applications.

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“…The paring of these two polymers improved the electrospinnability through strong hydrogen bonding between chitosan and PEO chains. Other popular natural polymers commonly used in blends for electrospinning in application of wound healing include alginate [ 36 ], gelatin [ 37 , 38 ], cellulose [ 39 ], collagen [ 40 , 41 ], hyaluronic acid [ 42 , 43 , 44 ], keratin [ 45 ], silk fibroin [ 46 ], and zein [ 47 , 48 ].…”

Section: Electrospun Fibersmentioning

confidence: 99%

Electrospun Fibers as a Dressing Material for Drug and Biological Agent Delivery in Wound Healing Applications

et al. 2018

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Wound healing is a complex tissue regeneration process that promotes the growth of new tissue to provide the body with the necessary barrier from the outside environment. In the class of non-healing wounds, diabetic wounds, and ulcers, dressing materials that are available clinically (e.g., gels and creams) have demonstrated only a slow improvement with current available technologies. Among all available current technologies, electrospun fibers exhibit several characteristics that may provide novel replacement dressing materials for the above-mentioned wounds. Therefore, in this review, we focus on recent achievements in electrospun drug-eluting fibers for wound healing applications. In particular, we review drug release, including small molecule drugs, proteins and peptides, and gene vectors from electrospun fibers with respect to wound healing. Furthermore, we provide an overview on multifunctional dressing materials based on electrospun fibers, including those that are capable of achieving wound debridement and wound healing simultaneously as well as multi-drugs loading/types suitable for various stages of the healing process. Our review provides important and sufficient information to inform the field in development of fiber-based dressing materials for clinical treatment of non-healing wounds.

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Chitosan-co-Hyaluronic acid porous cryogels and their application in tissue engineering

Cited by 84 publications

References 41 publications

Hyaluronic acid‐poly(vinyl alcohol) composite cryo‐gel for biofunctional material application

Hyaluronic acid‐poly(vinyl alcohol) composite cryo‐gel for biofunctional material application

Injectable Hyaluronic Acid-co-Gelatin Cryogels for Tissue-Engineering Applications

Electrospun Fibers as a Dressing Material for Drug and Biological Agent Delivery in Wound Healing Applications

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