Abstract:Chitosan/cellulose-based beads (CCBs) for the affinity purification of histidine-tagged proteins were prepared from chitosan/cellulose dissolved in ionic liquid as a solvent, and their structures were characterized by Fourier transform infrared spectroscopy, transmission electron microscopy, and thermogravimetric analysis. The affinity purification was used to separate hexahistidine-tagged (his-tagged) enhanced green fluorescent protein (EGFP) from Escherichia coli. The results showed that Zn-CCB exhibited mor… Show more
“…40,41 Simultaneously, absorption peaks at 3271–3287 cm −1 and 1589–1601 cm −1 attributed to the N–H stretching vibration and N–H bending vibration of amide groups decreased dramatically upon the addition of Cu 2+ ions. 42–44 However, the absorption band at 939–945 cm −1 which was ascribed to the out-of-plane bending vibration absorption of the carboxylic acid -OH group disappeared after the addition of Cu 2+ ions. 45 In addition, intense absorption bands at 1635–1643 cm −1 , corresponding to the adoption of β-sheet structures, shifted to higher frequencies (1647–1653 cm −1 ) in the spectra of the complex solution, implying the formation of random coil structures.…”
Modulating enzyme activities or functionalities is one of the primary features of biological systems, which is, however, a great challenge for artificial enzyme systems. In this work, we designed and...
“…40,41 Simultaneously, absorption peaks at 3271–3287 cm −1 and 1589–1601 cm −1 attributed to the N–H stretching vibration and N–H bending vibration of amide groups decreased dramatically upon the addition of Cu 2+ ions. 42–44 However, the absorption band at 939–945 cm −1 which was ascribed to the out-of-plane bending vibration absorption of the carboxylic acid -OH group disappeared after the addition of Cu 2+ ions. 45 In addition, intense absorption bands at 1635–1643 cm −1 , corresponding to the adoption of β-sheet structures, shifted to higher frequencies (1647–1653 cm −1 ) in the spectra of the complex solution, implying the formation of random coil structures.…”
Modulating enzyme activities or functionalities is one of the primary features of biological systems, which is, however, a great challenge for artificial enzyme systems. In this work, we designed and...
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