Chitosan-Based Systems for Gene Delivery

Sharma, Divya; Arora, Sanjay; Rodrigues, Bruna dos Santos; Lakkadwala, Sushant; Banerjee, Amrita; Singh, Jagdish

doi:10.1007/978-981-15-0263-7_8

Cited by 9 publications

(5 citation statements)

References 273 publications

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“…Chitosan with cationic polyelectrolyte character offers a stronger electrostatic interaction with the negatively charged nucleic acids and thus safeguards it from degradation by nuclease. 217 , 218 Nevertheless, due to its poor transfection efficacy, the in vivo applications of this polymer are restricted. As a result, scientists used different strategies to enhance the biomolecular transfection efficiency of chitosan.…”

Section: Targeted Mirna Delivery For Chronic Woundsmentioning

confidence: 99%

“…Several derivatives of chitosan can be employed for the targeted biomolecular delivery. Chitosan with cationic polyelectrolyte character offers a stronger electrostatic interaction with the negatively charged nucleic acids and thus safeguards it from degradation by nuclease 217,218 . Nevertheless, due to its poor transfection efficacy, the in vivo applications of this polymer are restricted.…”

Section: Targeted Mirna Delivery For Chronic Woundsmentioning

confidence: 99%

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miRNA‐encapsulated abiotic materials and biovectors for cutaneous and oral wound healing: Biogenesis, mechanisms, and delivery nanocarriers

Dey

Yousefiasl

Kumar

et al. 2022

Bioengineering & Transla Med

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MicroRNAs (miRNAs) as therapeutic agents have attracted increasing interest in the past decade owing to their significant effectiveness in treating a wide array of ailments. These polymerases II‐derived noncoding RNAs act through post‐transcriptional controlling of different proteins and their allied pathways. Like other areas of medicine, researchers have utilized miRNAs for managing acute and chronic wounds. The increase in the number of patients suffering from either under‐healing or over‐healing wound demonstrates the limited efficacy of the current wound healing strategies and dictates the demands for simpler approaches with greater efficacy. Various miRNA can be designed to induce pathway beneficial for wound healing. However, the proper design of miRNA and its delivery system for wound healing applications are still challenging due to their limited stability and intracellular delivery. Therefore, new miRNAs are required to be identified and their delivery strategy needs to be optimized. In this review, we discuss the diverse roles of miRNAs in various stages of wound healing and provide an insight on the most recent findings in the nanotechnology and biomaterials field, which might offer opportunities for the development of new strategies for this chronic condition. We also highlight the advances in biomaterials and delivery systems, emphasizing their challenges and resolutions for miRNA‐based wound healing. We further review various biovectors (e.g., adenovirus and lentivirus) and abiotic materials such as organic and inorganic nanomaterials, along with dendrimers and scaffolds, as the delivery systems for miRNA‐based wound healing. Finally, challenges and opportunities for translation of miRNA‐based strategies into clinical applications are discussed.

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Section: Targeted Mirna Delivery For Chronic Woundsmentioning

confidence: 99%

Section: Targeted Mirna Delivery For Chronic Woundsmentioning

confidence: 99%

miRNA‐encapsulated abiotic materials and biovectors for cutaneous and oral wound healing: Biogenesis, mechanisms, and delivery nanocarriers

Dey

Yousefiasl

Kumar

et al. 2022

Bioengineering & Transla Med

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“…The polyelectrolyte nature of chitosan allows it to bind to negatively charged DNA. Electrostatic interactions between the polycation chitosan and DNA solutions can yield the spontaneous formation of electrostatic self-assembled polyelectrolyte complexes (PECs) nanostructures, also denoted here as polyplexes or nanocomplexes, using a complex coacervation process [11][12][13]. The presence of the coulombic interactions between the oppositely charged polymers leads to the ionic condensation of the DNA during PEC formation and protection from nuclease degradation [10].…”

Section: Introductionmentioning

confidence: 99%

On the Fractionation and Physicochemical Characterisation of Self-Assembled Chitosan–DNA Polyelectrolyte Complexes

2023

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Chitosan is extensively studied as a carrier for gene delivery and is an attractive non-viral gene vector owing to its polycationic, biodegradable, and biocompatible nature. Thus, it is essential to understand the chemistry of self-assembled chitosan–DNA complexation and their structural and functional properties, enabling the formation of an effective non-viral gene delivery system. In this study, two parent chitosans (samples NAS-032 and NAS-075; Mw range ~118–164 kDa) and their depolymerised derivatives (deploy nas-032 and deploy nas-075; Mw range 6–14 kDa) with degrees of acetylation 43.4 and 4.7%, respectively, were used to form polyelectrolyte complexes (PECs) with DNA at varying [–NH3+]/ [−PO4−] (N/P) molar charge ratios. We investigated the formation of the PECs using ζ-potential, asymmetric flow field-flow fractionation (AF4) coupled with multiangle light scattering (MALS), refractive index (RI), ultraviolet (UV) and dynamic light scattering (DLS) detectors, and TEM imaging. PEC formation was confirmed by ζ-potential measurements that shifted from negative to positive values at N/P ratio ~2. The radius of gyration (Rg) was determined for the eluting fractions by AF4-MALS-RI-UV, while the corresponding hydrodynamic radius (Rh), by the DLS data. We studied the influence of different cross-flow rates on AF4 elution patterns for PECs obtained at N/P ratios 5, 10, and 20. The determined rho shape factor (ρ = Rg/Rh) values for the various PECs corresponded with a sphere morphology (ρ ~ 0.77–0.85), which was consistent with TEM images. The results of this study represent a further step towards the characterisation of chitosan–DNA PECs by the use of multi-detection AF4 as an important tool to fractionate and infer aspects of their morphology.

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“…Specifically, chitosan-based vectors have received great attention over the past decade [ 15 , 16 , 17 , 18 ]. Interest in chitosan-based vectors is mostly due to the ease of modification, biocompatibility, and biodegradability [ 19 , 20 , 21 ]. Although a promising delivery tool, gene transfections achieved with unmodified chitosan are not desirable [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Fatty Acid Grafted Chitosan Polymeric Micelles for Improved Gene Delivery of VGF to the Brain through Intranasal Route

et al. 2022

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Multifunctional fatty acid grafted polymeric micelles are an effective and promising approach for drug and gene delivery to the brain. An alternative approach to bypass the blood–brain barrier is administration through intranasal route. Multifunctional fatty acid grafted polymeric micelles were prepared and characterized for pVGF delivery to the brain. In vitro pVGF expression was analyzed in bEnd.3 cells, primary astrocytes, and neurons. Comparative in-vivo pVGF expression was analyzed to evaluate the effective route of administration between intranasal and intravenous. Biocompatible, multifunctional polymeric micelles were prepared, having an average size of 200 nm, and cationic zeta potential. Modified polymers were found to be hemo- and cyto-compatible. When transfected with the different modified chitosan formulations, significantly (p < 0.05) higher VGF expression was observed in primary astrocytes and neurons using the mannose, Tat peptide, and oleic acid grafted chitosan polymer. Compared to intravenous administration, intranasal administration of pVGF in polyplex formulation led to significantly (p < 0.05) higher pVGF expression. Developed multifunctional polymeric micelles were an effective pVGF delivery platform to the brain. Mannose and Tat ligand tagging improved the pVGF delivery to the brain.

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Chitosan-Based Systems for Gene Delivery

Cited by 9 publications

References 273 publications

miRNA‐encapsulated abiotic materials and biovectors for cutaneous and oral wound healing: Biogenesis, mechanisms, and delivery nanocarriers

miRNA‐encapsulated abiotic materials and biovectors for cutaneous and oral wound healing: Biogenesis, mechanisms, and delivery nanocarriers

On the Fractionation and Physicochemical Characterisation of Self-Assembled Chitosan–DNA Polyelectrolyte Complexes

Synthesis and Characterization of Fatty Acid Grafted Chitosan Polymeric Micelles for Improved Gene Delivery of VGF to the Brain through Intranasal Route

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