Chitosan-based siRNA delivery systems

Ragelle, Héloïse; Vandermeulen, Gaëlle; Préat, Véronique

doi:10.1016/j.jconrel.2013.08.005

Cited by 224 publications

(166 citation statements)

References 66 publications

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“…Interestingly, when they were subjected to different cell models and different experimental conditions a greater degree of variability in results was observed [68].…”

Section: Chitosanmentioning

confidence: 99%

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Functionalized non-viral cationic vectors for effective siRNA induced cancer therapy

Gupta

Puri

Shapiro

2017

DNA and RNA Nanotechnology

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RNA interference (RNAi) has been regarded as a vital asset in the field of therapeutics as it has the capability to silence various disease causing genes including those that cause cancer. Small non-coding RNA molecules such as short interfering RNAs (siRNAs) are one of the extensively studied RNAi inducers for gene modulations. However, the delivery of RNAi inducers including siRNAs is compromised due to the barriers imposed by the biological system such as degradation by nucleases, rapid clearance, high anionic charge, immunogenicity and off-target effects. Viral vectors, in general exhibit high transfection efficiencies but are expensive and likely to confer immunological and safety issues. Therefore, non-viral cationic vectors (NVCVs) have received considerable attention to not only address these issues but also for developing efficacious siRNA delivery vectors. In this review, we will first discuss the historical development of various NVCVs and then will discuss functionalized NVCVs with linkers that provide stability, as well as respond to the cancer cell environment and with cancer cell receptor specific ligands to explicitly target them for improved siRNA efficacy. Multifunctional NVCVs (MNVCVs) that employ multiple synergistically working components to aid siRNA delivery efficacy are also discussed.

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“…Interestingly, when they were subjected to different cell models and different experimental conditions a greater degree of variability in results was observed [68].…”

Section: Chitosanmentioning

confidence: 99%

“…However, the molecular weight, deacetylation degree of chitosan, formulation strategy with siRNAs and incorporation of membrane permeating, endosomolytic and targeting ligands also play a significant role in efficient gene silencing [68].…”

Section: Chitosanmentioning

confidence: 99%

Functionalized non-viral cationic vectors for effective siRNA induced cancer therapy

Gupta

Puri

Shapiro

2017

DNA and RNA Nanotechnology

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“…So CS is usually used as a safe and efficient vector for siRNA or DNA delivery. 28 However, CS has lower solubility that leads to instability of CS nanoparticles in physiological conditions. In order to increase the solubility of CS and prepare stable CS nanoparticles, PEG was selectively grafted onto the hydroxyl groups of CS to produce PEG-CS copolymer.…”

Section: Animal Experimentsmentioning

confidence: 99%

Chitosan-based nanoparticles for survivin targeted siRNA delivery in breast tumor therapy and preventing its metastasis

Park¹,

Huang

Jin³

et al. 2016

IJN

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Nanoparticle-mediated small interfering RNA (siRNA) delivery is a promising therapeutic strategy in various cancers. However, it is difficult to deliver degradative siRNA to tumor tissue, and thus a safe and efficient vector for siRNA delivery is essential for cancer therapy. In this study, poly(ethylene glycol)-modified chitosan (PEG-CS) was synthesized successfully for delivering nucleic acid drug. We deemed that PEGylated CS could improve its solubility by forming a stable siRNA loaded in nanoparticles, and enhancing transfection efficiency of siRNA-loaded CS nanoparticles in cancer cell line. The research results showed that siRNA loaded in PEGylated CS (PEG-CS/siRNA) nanoparticles with smaller particle size had superior structural stability in the physical environment compared to CS nanoparticles. The data of in vitro antitumor activity revealed that 4T1 tumor cell growth was significantly inhibited and cellular uptake of PEG-CS/siRNA nanoparticles in 4T1 cells was dramatically enhanced compared to naked siRNA groups. The results from flow cytometry and confocal laser scanning microscopy showed that PEG-CS/siRNA nanoparticles were more easily taken up than naked siRNA. Importantly, PEG-CS/siRNA nanoparticles significantly reduced the growth of xenograft tumors of 4T1 cells in vivo. It has been demonstrated that the PEG-CS is a safe and efficient vector for siRNA delivery, and it can effectively reduce tumor growth and prevent metastasis.

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“…Chitosan is also used as a food additive and preservative in food processing [3]. Recent advances in the use of this abundant biopolymer in the field of medical sciences includes the production of nanoparticles used in drug delivery, gene delivery, tissues engineering and in vivo DNA targeting [7,8]. Generally, the efficiency of chitosan's application depends on its physiological pH, degree of deacetylation and its molecular weight [1,3,9].…”

Section: Introductionmentioning

confidence: 99%

Chitosan multiple addition enhances laccase production from Trametes versicolor

Adekunle

Wang

et al. 2015

Bioprocess Biosyst Eng

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Chitosan multiple addition strategy was developed to improve laccase production from Trametes versicolor cultures. The optimized multiple addition strategy was carried out by two-time addition of 0.1 g L -1 chitosan to a 2-day-old culture media, with 24-h interval between the treatments. Under these conditions, laccase activity of 644.9 U l -1 was achieved on the seventh day and laccase production was improved by 93.5 % higher than the control. Chitosan treatment increased reactive oxygen species generation and extracellular protein concentration in the treated mycelia. In contrast, the inducer inhibited the mycelia growth. The result of the quantitative reverse transcription polymerase chain reaction showed that the copy number of the laccase gene transcript increased by 16.7-fold in the treated mycelia relative to the control. This study provides insight into some of the intrinsic metabolic processes involved in the upregulation of laccase production in the presence of chitosan inducer in fungal culture.

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Chitosan-based siRNA delivery systems

Cited by 224 publications

References 66 publications

Functionalized non-viral cationic vectors for effective siRNA induced cancer therapy

Functionalized non-viral cationic vectors for effective siRNA induced cancer therapy

Chitosan-based nanoparticles for survivin targeted siRNA delivery in breast tumor therapy and preventing its metastasis

Chitosan multiple addition enhances laccase production from Trametes versicolor

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