Chitosan-based scaffolds for the support of smooth muscle constructs in intestinal tissue engineering

Zakhem, Elie; Raghavan, Shreya; Gilmont, Robert R.; Bitar, Khalil N.

doi:10.1016/j.biomaterials.2012.03.051

Cited by 88 publications

(69 citation statements)

References 40 publications

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“…A low toxicity among cells cultured on chitosan/collagen nanoscaffolds has been noted in other studies on culture of bone (Levengood & Zhang, 2014), cartilage (Gong et al, 2010), skin (Sarkar et al, 2013), intestinal smooth muscle (Zakhem et al, 2012) and endothelial cells (Swarnalatha et al, 2013).…”

Section: Discussionmentioning

confidence: 88%

Effects of 3-dimensional culture conditions (collagen-chitosan nano-scaffolds) on maturation of dendritic cells and their capacity to interact with T-lymphocytes

Daneshmandi

Dibazar

Fateh

2015

Journal of Immunotoxicology

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In the body, there is a natural three-dimensional (3D) microenvironment in which immune cells, including dendritic cells (DC), play their functions. This study evaluated the impact of using collagen-chitosan 3D nano-scaffolds in comparisons to routine 2D culture plates on DC phenotype and functions. Bone marrow-derived DC were cultured on scaffolds and plates and then stimulated with lipopolysaccharide (LPS) or chitosan-based nanoparticles (NP) for 24 h. Thereafter, DC viability, expression of maturation markers and levels of cytokines secretion were evaluated. In another set of studies, the DC were co-cultured with allogenic T-lymphocytes in both the 2D and 3D systems and effects on DC-induction of T-lymphocyte proliferation and cytokine release were analyzed. The results indicated that CD40, CD86 and MHC II marker expression and interleukin (IL)-12, IL-6 and tumor necrosis factor (TNF)-secretion by DC were enhanced in 3D cultures in comparison to by cells maintained in the 2D states. The data also showed that DNA/chitosan NP activated DC more than LPS in the 3D system. T-Lymphocyte proliferation was induced to a greater extent by DNA/NP-treated DC when both cell types were maintained on the scaffolds. Interestingly, while DC induction of T-lymphocyte interferon (IFN)-and IL-4 release was enhanced in the 3D system (relative to controls), there was a suppression of transforming growth factor (TGF)-production; effects on IL-10 secretion were variable. The results here suggested that collagen-chitosan scaffolds could provide a proinflammatory and activator environment to perform studies to analyze effects of exogenous agents on the induction of DC maturation, NP uptake and/or cytokines release, as well as for the ability of these cells to potentially interact with other immune system cells in vitro.

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Section: Discussionmentioning

confidence: 88%

Effects of 3-dimensional culture conditions (collagen-chitosan nano-scaffolds) on maturation of dendritic cells and their capacity to interact with T-lymphocytes

Daneshmandi

Dibazar

Fateh

2015

Journal of Immunotoxicology

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“…[17][18][19][20] In advancing these technologies, it is important to be able to accurately characterize the cell response using molecular biology techniques including RT-PCR analysis of gene expression. These types of studies will provide critical insight into how engineered cellular microenvironments can be tuned to optimize the cell response for each specific application.…”

Section: Discussionmentioning

confidence: 99%

Techniques for the Isolation of High-Quality RNA from Cells Encapsulated in Chitosan Hydrogels

Young

Russo

et al. 2013

Tissue Engineering Part C: Methods

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Extracting high-quality RNA from hydrogels containing polysaccharide components is challenging, as traditional RNA isolation techniques designed for cells and tissues can have limited yields and purity due to physiochemical interactions between the nucleic acids and the biomaterials. In this study, a comparative analysis of several different RNA isolation methods was performed on human adipose-derived stem cells photoencapsulated within methacrylated glycol chitosan hydrogels. The results demonstrated that RNA isolation methods with cetyl trimethylammonium bromide (CTAB) buffer followed by purification with an RNeasy Ò mini kit resulted in low yields of RNA, except when the samples were preminced directly within the buffer. In addition, genomic DNA contamination during reverse transcriptase-polymerase chain reaction (RT-PCR) analysis was observed in the hydrogels processed with the CTAB-based methods. Isolation methods using TRIzol Ò in combination with one of a Qiaex Ò gel extraction kit, an RNeasy Ò mini kit, or an extended solvent purification method extracted RNA suitable for gene amplification, with no evidence of genomic contamination. The latter two methods yielded the best results in terms of yield and amplification efficiency. Predigestion of the scaffolds with lysozyme was investigated as a possible means of enhancing RNA extraction from the polysaccharide gels, with no improvements observed in terms of the purity, yield, or amplification efficiency. Overall, this work highlights the application of a TRIzol Ò + extended solvent purification method for optimizing RNA extraction that can be applied to obtain reliable and accurate gene expression data in studies investigating cells seeded in chitosan-based scaffolds.

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“…The choice of the cell source is a big challenge in TE because of the limited availability of autologous cell sources. Additionally, it is very important to isolate enough cells and expand them in vitro for seeding onto the biomaterial (Zakhem et al 2012). …”

Section: Sct and Tementioning

confidence: 99%

Intestinal stem cells and stem cell-based therapy for intestinal diseases

2014

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Currently, many gastrointestinal diseases are a major reason for the increased mortality rate of children and adults every year. Additionally, these patients may cope with the high cost of the parenteral nutrition (PN), which aids in the long-term survival of the patients. Other treatment options include surgical lengthening, which is not sufficient in many cases, and intestinal transplantation. However, intestinal transplantation is still accompanied by many challenges, including immune rejection and donor availability, which may limit the transplant's success. The development of more safe and promising alternative treatments for intestinal diseases is still ongoing. Stem cell-based therapy (SCT) and tissue engineering (TE) appear to be the next promising choices for the regeneration of the damaged intestine. However, suitable stem cell source is required for the SCT and TE process. Thus, in this review we discuss how intestinal stem cells (ISCs) are a promising cell source for small intestine diseases. We will also discuss the different markers were used to identify ISCs. Moreover, we discuss the dominant Wnt signaling pathway in the ISC niche and its involvement in some intestinal diseases. Additionally, we discuss ISC culture and expansion, which are critical to providing enough cells for SCT and TE. Finally, we conclude and recommend that ISC isolation, culture and expansion should be considered when SCT is a treatment option for intestinal disorders. Therefore, we believe that ISCs should be considered a cell source for SCT for many gastrointestinal diseases and should be highlighted in future clinical applications.

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Chitosan-based scaffolds for the support of smooth muscle constructs in intestinal tissue engineering

Cited by 88 publications

References 40 publications

Effects of 3-dimensional culture conditions (collagen-chitosan nano-scaffolds) on maturation of dendritic cells and their capacity to interact with T-lymphocytes

Effects of 3-dimensional culture conditions (collagen-chitosan nano-scaffolds) on maturation of dendritic cells and their capacity to interact with T-lymphocytes

Techniques for the Isolation of High-Quality RNA from Cells Encapsulated in Chitosan Hydrogels

Intestinal stem cells and stem cell-based therapy for intestinal diseases

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