Chitosan based oligoamine polymers: Synthesis, characterization, and gene delivery

Lü, Bo; Wang, Chang-Fang; Wu, Dequn; Cao, Li; Zhang, Xian‐Zheng; Zhuo, Ren‐Xi

doi:10.1016/j.jconrel.2009.03.004

Cited by 104 publications

(69 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Accordingly, as observed in Figure 1, our data indicate that UOCs have significantly higher buffer capacity compared to the high-MW chitosan (G214), which is more significant in the pH interval between 9 and 3. This is in agreement with a study that showed that the buffer capacity of chitosan copolymers decreased when the MW of chitosan was increased (Lu et al, 2009). …”

Section: Buffer Capacity Of Uocssupporting

confidence: 93%

“…It has been reported that the capacity to escape from the endosome depends on the intrinsic buffer capacity of the vector system (Tripathi et al, 2012). As reported by the literature, chitosans have a modest buffering capacity comparing with other cationic synthetic polymers, such as chitosan-based oligoamine polymers and polyethylenimine, which reduces its biomedical applications (Lu et al, 2009).…”

Section: Buffer Capacity Of Uocsmentioning

confidence: 99%

“…Despite the positive superficial charge (around þ15 mV) of our UOC-based carriers, we suggest that they have enough ability for proton absorption, facilitating the rupture of the endosomal vesicles and the release of the polyplex into the cytoplasm. The lack of transfection observed with G214 polyplexes could be explained by the low buffer ability of G214 chitosan, which enables endosomal escape (Panyam & Labhasetwar, 2003;Lu et al, 2009).…”

Section: Influence Of Ph Decrease On the Transfection Efficiency Of Umentioning

confidence: 99%

“…Polyplexes elaborated with high-MW chitosans are very stable and excellent extracellular DNA protectors, but they show, at the same time, low buffer capacity and transfection efficiency (Lu et al, 2009). According to the DDA, high-DDA chitosan vectors have shown better DNA-binding capacity and more efficient transfection than complexes formed with low-DDA chitosan (Kim et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Improving transfection efficiency of ultrapure oligochitosan/DNA polyplexes by medium acidification

et al. 2014

View full text Add to dashboard Cite

Context: Ultrapure oligochitosans (UOCs) have recently been reported as efficient nonviral vectors for corneal and retinal gene delivery. However, the influence of some physicochemical factors on the transfection efficiency, such as the pH, remains unclear. Deeper in vitro research of these factors could provide valuable information for future clinical applications. Objective: The aim of this study is to determine the influence of the pH decrease on the transfection efficiency of UOC/pDNA polyplexes in HEK293 and ARPE19 cells. Materials and methods: We elaborated self-assembled UOC/pCMS-EGFP polyplexes. The influence of the most important factors on the particle size and the zeta potential was studied by an orthogonal experimental design. We evaluated, in vitro, the cellular uptake and the transfection efficiency by flow cytometry, and the cytotoxicity of the vectors by CCK-8 assay.Results and discussion: The pH of the medium strongly influences the physicochemical properties of the polyplexes, and by its modulation we are able to control their superficial charge. A significant increase on the cellular uptake and transfection efficiency of UOCs was obtained when the pH was acidified. Neither of our UOC/pCMS-EGFP polyplexes caused cytotoxicity; however, cells treated with Lipofectamine 2000ä showed decreased cell viability. Conclusion: This kind of UOC vectors could be useful to transfect cells that are in an acidic environment, such as tumor cells. However, additional in vivo studies may be required in order to obtain an effective and safe medicine for nonviral gene therapy purpose.

show abstract

Section: Buffer Capacity Of Uocssupporting

confidence: 93%

Section: Buffer Capacity Of Uocsmentioning

confidence: 99%

Section: Influence Of Ph Decrease On the Transfection Efficiency Of Umentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Improving transfection efficiency of ultrapure oligochitosan/DNA polyplexes by medium acidification

et al. 2014

View full text Add to dashboard Cite

show abstract

“…This ion influx causes an increase in the osmotic pressure inside the vesicle and thus a swelling process ruptures the endosome/lysosome, allowing the escape of the polyplexes before being degradated ( Figure 5). The buffering capacity of Ch has been determined by acid-base titration assay [30,44]. Some authors have concluded that it is unlikely that Ch escape the endosome by proton sponge effect due to its low buffering capacity compared to other cationic polymers with high buffering capacity, such as polyethilenimine (PEI).…”

Section: Buffering Capacitymentioning

confidence: 99%

Low Molecular Weight Chitosan (LMWC)-based Polyplexes for pDNA Delivery: From Bench to Bedside

et al. 2014

View full text Add to dashboard Cite

Non-viral gene delivery vectors are emerging as a safer alternative to viral vectors. Among natural polymers, chitosan (Ch) is the most studied one, and low molecular weight Ch, specifically, presents a wide range of advantages for non-viral pDNA delivery. It is crucial to determine the best process for the formation of Low Molecular Weight Chitosan (LMWC)-pDNA complexes and to characterize their physicochemical properties to better understand their behavior once the polyplexes are administered. The transfection efficiency of Ch based polyplexes is relatively low. Therefore, it is essential to understand all the transfection process, including the cellular uptake, endosomal escape and nuclear import, together with the parameters involved in the process to improve the design and development of the non-viral vectors. The aim of this review is to describe the formation and characterization of LMWC based polyplexes, the in vitro transfection process and finally, the in vivo applications of LMWC based polyplexes for gene therapy purposes.

show abstract

Polymeric Gene Carriers

Chen

Tian

Guan

2014

Bioinspired and Biomimetic Polymer Systems for Drug and Gene Delivery

View full text Add to dashboard Cite

Chitosan based oligoamine polymers: Synthesis, characterization, and gene delivery

Cited by 104 publications

References 44 publications

Improving transfection efficiency of ultrapure oligochitosan/DNA polyplexes by medium acidification

Improving transfection efficiency of ultrapure oligochitosan/DNA polyplexes by medium acidification

Low Molecular Weight Chitosan (LMWC)-based Polyplexes for pDNA Delivery: From Bench to Bedside

Polymeric Gene Carriers

Contact Info

Product

Resources

About