Chitosan-based Maillard products for enzyme immobilization in multilayers structure: Its application in electrochemical sensing

Gulotta, Florencia Alejandra; Montenegro, Mariana; Diaz, Ladislao Vergara; Badano, Joaquín Arata; Ferreyra, Nancy F.; Zanini, Verónica I. Paz

doi:10.1016/j.microc.2023.108689

Cited by 5 publications

(2 citation statements)

References 34 publications

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“…The sensitivity and reusability of the biosensor were analyzed. The average values of sensitivity of the three independent measurements; the coefficient of variation of the sensitivity; and the linear range were (0.220 ± 0.002) mA mM −1 , 9%, (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15) µM and (0.190 ± 0.001) mA mM −1 , 5%, (1-9) µM for the 4:1 and 20:1 composites, respectively. Both composites were used five days after their preparation to modify electrodes and to adsorb HRP from the fresh protein solution.…”

Section: Selection Of the Composite Proportion And Hrp Adsorption Met...mentioning

confidence: 94%

“…In this context, the Maillard reaction (MR) emerges as a safe synthesis methodology that can obtain CHIT derivatives with improved water solubility [ 8 , 9 ]. Despite their improved properties [ 10 ], their use in the design and development of electrochemical biosensing platforms is a recent area of interest aimed at capitalizing on their notable characteristics [ 11 ]. Due to their hydrophilic nature, CHIT derivatives obtained by MR can be used as dispersing or surface-functionalization agents of nanomaterials to obtain nanohybrid composites with synergic and improved properties.…”

Section: Introductionmentioning

confidence: 99%

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Immobilization of Horseradish Peroxidase onto Montmorillonite/Glucosamine–Chitosan Composite for Electrochemical Biosensing of Polyphenols

Piccoli,

Gulotta,

Montenegro

et al. 2024

Biosensors

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Glucosamine–chitosan synthesized by the Maillard reaction was combined with montmorillonite to obtain a nanohybrid composite to immobilize horseradish peroxidase. The material combines the advantageous properties of clay with those of the chitosan derivative; has improved water solubility and reduced molecular weight and viscosity; involves an eco-friendly synthesis; and exhibits ion exchange capacity, good adhesiveness, and a large specific surface area for enzyme adsorption. The physicochemical characteristics of the composite were analyzed by infrared spectroscopy and X-ray diffraction to determine clay–polycation interactions. The electrochemical response of the different polyphenols to glassy carbon electrodes modified with the composite was evaluated by cyclic voltammetry. The sensitivity and detection limit values obtained with the biosensor toward hydroquinone, chlorogenic acid, catechol, and resorcinol are (1.6 ± 0.2) × 102 µA mM−1 and (74 ± 8) nM; (1.2 ± 0.1) × 102 µA mM−1 and (26 ± 3) nM; (16 ± 2) µA mM−1 and (0.74 ± 0.09) μM; and (3.7± 0.3) µA mM−1 and (3.3 ± 0.2) μM, respectively. The biosensor was applied to quantify polyphenols in pennyroyal and lemon verbena extracts.

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Section: Selection Of the Composite Proportion And Hrp Adsorption Met...mentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Immobilization of Horseradish Peroxidase onto Montmorillonite/Glucosamine–Chitosan Composite for Electrochemical Biosensing of Polyphenols

Piccoli,

Gulotta,

Montenegro

et al. 2024

Biosensors

Self Cite

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A critical review of enzymes immobilized on chitosan composites: characterization and applications

Bai

Jing

et al. 2023

Bioprocess Biosyst Eng

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Optical and electric properties of the organic-inorganic hybrid bis(2-amino-5-picolinium) Tetrachlorocobaltate(II) [(C6H9N2)2CoCl4]

Ghoudi,

Chaabane,

Naouari

et al. 2024

Inorganic Chemistry Communications

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Chitosan-based Maillard products for enzyme immobilization in multilayers structure: Its application in electrochemical sensing

Cited by 5 publications

References 34 publications

Immobilization of Horseradish Peroxidase onto Montmorillonite/Glucosamine–Chitosan Composite for Electrochemical Biosensing of Polyphenols

Immobilization of Horseradish Peroxidase onto Montmorillonite/Glucosamine–Chitosan Composite for Electrochemical Biosensing of Polyphenols

A critical review of enzymes immobilized on chitosan composites: characterization and applications

Optical and electric properties of the organic-inorganic hybrid bis(2-amino-5-picolinium) Tetrachlorocobaltate(II) [(C6H9N2)2CoCl4]

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