Chitosan-based films with cannabis oil as a base material for wound dressing application

Abstract

This study focuses on obtaining and characterizing novel chitosan-based biomaterials containing cannabis oil to potentially promote wound healing. The primary active substance in cannabis oil is the non-psychoactive cannabidiol, which has many beneficial properties. In this study, three chitosan-based films containing different concentrations of cannabis oil were prepared. As the amount of oil increased, the obtained biomaterials became rougher as tested by atomic force microscopy. Such rough surfaces promote protein adsorption, confirmed by experiments assessing the interaction between human albumin with the obtained materials. Increased oil concentration also improved the films' mechanical parameters, swelling capacity, and hydrophilic properties, which were checked by the wetting angle measurement. On the other hand, higher oil content resulted in decreased water vapour permeability, which is essential in wound dressing. Furthermore, the prepared films were subjected to an acute toxicity test using a Microtox. Significantly, the film's increased cannabis oil content enhanced the antimicrobial effect against A. fischeri for films in direct contact with bacteria. More importantly, cell culture studies revealed that the obtained materials are biocompatible and, therefore, they might be potential candidates for application in wound dressing materials.

In vivo and in silico studies of the effects of oil extracted from Cannabis sativa L. seeds on healing of burned skin wounds in rats

“…No significant changes in ASAT, creatinine, or urea ( p > 0.05) were observed among the treatments, including the negative control group, the SSD group, and the CSO group ( Table 3 ), which indicated no adverse effects on either liver or kidney function. These findings are supported by previous studies ( Hartsel et al, 2019 ; Chelminiak-Dudkiewicz et al, 2022 ). However, significantly ( p < 0.01) greater concentrations of ALAT were observed in rats treated with SSD than in the negative control rats.…”

Cannabidiol attenuates inflammatory impairment of intestinal cells expanding biomaterial-based therapeutic approaches

“…Cannabidiol (CBD) has received growing attention in recent years because of its potential therapeutic properties for various ailments, including chronic pain, anxiety, and epilepsy [ 41 ]. It has been combined with other biomaterials such as chitosan and alginate-based hydrogel [ 4 , 5 ] and is a promising compound for the development of new therapeutic options aimed at restoring a disturbed barrier resulting from inflammatory conditions [ 42 , 43 ]. Despite its increasing popularity, much research is still needed if we are fully to understand the potential benefits and risks of using CBD to treat various health conditions.…”

“…In recent years, cannabidiol (CBD), one of the main secondary plant compounds of Cannabis sativa L., has been established as a novel compound for intestinal drug targeting and development [ [1] , [2] , [3] ]. Moreover, a combination of CBD with other biomaterials such as chitosan and alginate-based hydrogel has gained major attention [ 4 , 5 ]. The historical use of Cannabis sativa for various ailments, such as nausea, vomiting, diarrhea, and pain, dates back to antiquity [ 6 ].…”

Sustainable Silk-Based Particulate Systems for the Controlled Release of Pharmaceuticals and Bioactive Agents in Wound Healing and Skin Regeneration

“…Silver nanoparticles have been shown to have better antimicrobial properties than bulk metal and can potentially overcome some of the limitations seen with current solutions, but they are unstable in aqueous environments [ 135 ]. Similarly to SS, chitosan has also demonstrated wound-healing effects [ 135 , 136 ]. For these reasons, Verma et al [ 135 ] investigated a sericin/chitosan-capped silver nanoparticle system for wound healing applications, where NPs were prepared via the chemical reduction of silver nitrate using chitosan and sericin as the stabilizing polymers.…”