Chitosan-based delivery systems for protein therapeutics and antigens

Amidi, Maryam; Mastrobattista, Enrico; Jiskoot, Wim; Hennink, Wim E.

doi:10.1016/j.addr.2009.11.009

Cited by 557 publications

(304 citation statements)

References 198 publications

Supporting

Mentioning

289

Contrasting

Unclassified

Order By: Relevance

“…TMC nanoand micro-particles have been fabricated by ionic cross-linking with different polyanions, such as polyglutamic acid, TPP, sodium sulfate, dextran sulfate, and anionic cyclodextrins. 18 The acidic environment of the stomach and enzymes pose significant challenges to the oral administration of vaccines. Figure 2.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Enteric trimethyl chitosan nanoparticles containing hepatitis B surface antigen for oral delivery

Farhadian

Dounighi

Avadi³

2015

Human Vaccines & Immunotherapeutics

View full text Add to dashboard Cite

Oral vaccination is the preferred route of immunization. However, the degradative condition of the gastrointestinal tract and the higher molecular size of peptides pose major challenges in developing an effective oral vaccination system. One of the most excellent methods used in the development of oral vaccine delivery system relies on the entrapment of the antigen in polymeric nanoparticles. In this work, trimethyl chitosan (TMC) nanoparticles were fabricated using ionic gelation teqnique by interaction hydroxypropyl methylcellulose phthalate (HPMCP), a pHsensitive polymer, with TMC and the utility of the particles in the oral delivery of hepatitis B surface antigen (HBsAg) was evaluated employing solutions that simulated gastric and intestinal conditions. The particle size, morphology, zeta potential, loading capacity, loading efficiency, in vitro release behavior, structure, and morphology of nanoparticles were evaluated, and the activity of the loaded antigen was assessed. Size of the optimized TMC/HPMCP nanoparticles and that of the antigen-loaded nanoparticles were 85 nm and 158 nm, respectively. Optimum loading capacity (76.75%) and loading efficiency (86.29%) were achieved at 300 mg/mL concentration of the antigen. SEM images revealed a spherical shape as well as a smooth and near-homogenous surface of nanoparticles. Results of the in vitro release studies showed that formulation with HPMCP improved the acid stability of the TMC nanoparticles as well as their capability to preserve the loaded HBsAg from gastric destruction. The antigen showed good activity both before and after loading. The results suggest that TMC/HPMCP nanoparticles could be used in the oral delivery of HBsAg vaccine.

show abstract

Section: Discussionmentioning

confidence: 99%

“…However, limited solubility has prevented its widespread use. 9,10 The mucoadhesive and absorption-promoting properties of chitosan have been extensively evaluated. The positive charge gained in acidic pH conditions, in which chitosan has high solubility, appears to be effective for enhanced absorption.…”

Section: Introductionmentioning

confidence: 99%

Enteric trimethyl chitosan nanoparticles containing hepatitis B surface antigen for oral delivery

Farhadian

Dounighi

Avadi³

2015

Human Vaccines & Immunotherapeutics

View full text Add to dashboard Cite

show abstract

“…The primary amine groups introduce special properties that render chitosan very useful for pharmaceutical applications. Because of the presence of functional groups (amine and hydroxyl) various chemical chitosan derivatives have been synthesized and studied for different applications [3,4].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Chitosan and fabrication of Chitosan hydrogels matrices for biomedical applications

Charhouf

Benaamara²,

Abourriche³

et al. 2013

MATEC Web of Conferences

View full text Add to dashboard Cite

Abstract. The development of injectable hydrogels for drug delivery is a major challenge. Chitosan is a copolymer of Nacetylglucosamine and glucosamine units and is represented as a copolymer. Chitosan occurs in nature in the cell walls of some fungi, exoskeletons of insects and marine animals such as crabs and prawns. Chitosan and its derivatives possess a wide range of useful properties. They are biodegradable, and biocompatible with antibacterial and antioxidant activities. They are useful in drug delivery formulations and tissue engineering. The objective of the present study was to characterize chitosan, prepare chitosan hydrogels and study the gelation of this hydrogels over time. Chitosan with DDA% ∼ 80%-90% were characterized by infrared spectroscopy, conductimetry and pH-metry. In addition, chitosan hydrogels were prepared using an ionic gelation method making it suitable for biomedical applications.

show abstract

“…Lysostaphin, a zinc metalloenzyme in S. simulans (Schindler & Schuhardt, 1964), is lytic against S.aureus cells, and has shown high potentials in the treatment of antibiotic-resistant S.aureus (Bhakta et al, 2003) and methicillin-resistant S.aureus (MRSA) infections (Dajcs et al, 2000). However, Amidi et al (2010) reviewed that the short residence time of the current formulations in the nasal cavity due to mucociliary clearance posed a severe limitation for the drug delivery in the nasal cavity. Kokai-Kun et al (2003) and Walsh et al (2004) prepared a semi-solid cream and found that the cream greatly enhanced the efficacy of lysostaphin against S.aureus nasal colonization in cotton rats.…”

Section: Introductionmentioning

confidence: 99%

Extension of nasal anti-Staphylococcus aureus efficacy of lysostaphin by its incorporation into a chitosan-o/w cream

Cui

Li²,

Huang³

et al. 2010

Drug Delivery

View full text Add to dashboard Cite

Chitosan-based delivery systems for protein therapeutics and antigens

Cited by 557 publications

References 198 publications

Enteric trimethyl chitosan nanoparticles containing hepatitis B surface antigen for oral delivery

Enteric trimethyl chitosan nanoparticles containing hepatitis B surface antigen for oral delivery

Characterization of Chitosan and fabrication of Chitosan hydrogels matrices for biomedical applications

Extension of nasal anti-Staphylococcus aureus efficacy of lysostaphin by its incorporation into a chitosan-o/w cream

Contact Info

Product

Resources

About