Chitosan as a wall material for a microencapsulated delivery system for Macrobrachium rosenbergii (de Man) larvae

Abstract

Chitosan has been widely accepted as a wall material for preparing microcapsules of various purposes in human medicine. The possibility of using chitosan as a wall material for microencapsulating nutrients and drugs for aquaculture purposes, specifically to Macrobrachium rosenbergii larvae was evaluated in this study. Two types of chitosan‐coated microcapsules were prepared using either acetone (MEC‐A) or NaOH (MEC‐N) as the cross‐linking agents. They were compared with a microbound diet relative to total leaching of nutrients and free amino acids (FAA). Among the microcapsules, MEC‐N showed the lowest level of total leaching of nutrients (23.3%) during 5 h of immersion in seawater and released 65% FAA after 60 min. During laboratory trials, 75% larvae had accepted the MEC‐N capsule. The results of the study suggest that chitosan can be used as a wall material for preparing microcapsules to deliver drugs and nutrients to M. rosenbergii larvae.

Gene Silencing in Crustaceans: From Basic Research to Biotechnologies

“…Mortality rates were reduced by 37% and 68% in the dsRNA-producing bacteria and dsRNA-chitosan nanoparticles—fed individuals, respectively. Anas et al [ 98 ] further characterized the properties of chitosan nanoparticles and concluded that they are suitable for encapsulating drugs for oral delivery into M. rosenbergii larvae. While this approach proved useful to some extent, only one very recent report has examined the option of using oral delivery to silence a gene systemically in crustaceans (summarized in Section 3.3 ).…”

<b>A Lipid-walled microcapsule diet as co-feed for early feeding the <i>Osphronemus gourami</i> (Lacepede) larvae

“…Microbound diet was also used as an alternative for larvae of Litopenaeus vannamei (D'Abramo, Perez, Sangha, & Puello-Cruz, 2006). Chitosan-walled microcapsule diet to know the morphology of capsule, leaching of total nutrients and free amino acids (FAA) and diet acceptance of Macrobrachium rosenbergii (Anas, Philip, & Singh, 2008). Gelatin-walled microcapsule diet was evaluated to know the retention of vitamin C, lipid encapsulation and nitrogen retention efficiency for Penaeus japonicus Bate (Xie et al, 2010).…”

Encapsulation for delivering bioactives in aquaculture

“…; Ozkizilcik & Chu ; Buchal & Langdon ; Baskerville‐Bridges & Kling ; Langdon ; Önal & Langdon ; Anas et al . ). Vitamin C is an example that was successfully preserved and delivered to rainbow trout via encapsulation (Alishahi et al .…”

“…For instance, chitosan was successfully used to produce a microencapsulated diet for marine larvae (Anas et al . ). Low level of nutrient leaching (23.3%) and high acceptance rate of 75% were reported for chitosan‐walled microcapsules after feeding Macrobrahium rosenbergii larvae (Anas et al .…”

“…Low level of nutrient leaching (23.3%) and high acceptance rate of 75% were reported for chitosan‐walled microcapsules after feeding Macrobrahium rosenbergii larvae (Anas et al . ). Utilising chitosan as a coating layer, not only it can be beneficial to confer stability to particles at basic pH of sea water, but it can also provide some levels of protection to the particles due to its antibacterial characteristics.…”