Chitosan as a vehicle for growth factor delivery: Various preparations and their applications in bone tissue regeneration

Venkatesan, Jayachandran; Anil, Sukumaran; Kim, Se‐Kwon; Shim, Min Suk

doi:10.1016/j.ijbiomac.2017.01.072

Cited by 71 publications

(40 citation statements)

References 112 publications

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“…Despite the slightly weakness of the inhibitory effects, LDL-RSG complex and LDL-RSG/CSNP could also significantly inhibit TGF-β1-stimulated HTF proliferation and ECM synthesis in HTFs similar as RSG, shown as down-regulated HTF cell viability, DNA synthesis capability and reduced α-SMA and Collagen I protein levels. In the present study, highly expressing LDLr in HTFs together with LDL helps to target the rightful release site of RSG; cationic polyelectrolyte chitosan solution and anionic calcium alginate solution were mixed and stirred to produce CSNP, which is stable, non-toxic and good film-forming [ 25 , 26 ], thus to steadily and targeted prolong the duration of RSG function in the postoperative operation area with minimum eye tissues toxic.…”

Section: Discussionmentioning

confidence: 99%

Low density lipoprotein - rosiglitazone - chitosan-calcium alginate/nanoparticles inhibition of human tenon's fibroblasts activation and proliferation

et al. 2017

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Anti-fibrotic therapeutic methods with safety and efficiency after glaucoma filtration surgery (GFS) are desirable. In our previous study, by using Human Tenon's Fibroblasts (HTFs) as a model, we proved the expression of peroxisome proliferator activates receptor-γ (PPAR-γ) in HTFs; in addition, rosiglitazone (RSG), an agonist of PPAR-γ, can inhibit transforming growth factorsβ1 (TGF-β1)-induced reactivation of HTFs, thus to inhibit specifically scarring after GFS through intervening TGF-β/Smads signal pathway. However, a better drug delivery way of RSG, to prolong the duration of its function, and to reduce the toxicity of RSG to ocular tissue still remains challenges. Low density lipoprotein receptor (LDLr) is strongly expressed in hyper-proliferation HTFs after GFS. Therefore, we structured targeting LDL-RSG complexes and channel them into HTFs through LDL-LDLr pathway in order to promote anti-proliferation of HTFs and reduce the toxicity to ocular tissue. Meanwhile, in order to improve the release properties of LDL-RSG complexes, we structured slow release system of LDL-RSG/chitosan-calcium alginate - nanoparticles (CSNP), which effectively inhibited TGF-β1-induced HTFs proliferation, synthesis of extracellular matrix and activation of TGF-β1/SMAD pathway. These data suggested that LDL-RSG/CSNP can be a new anti-fibrotic therapeutic method on scarring after GFS and also a novelty administration of RSG.

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Section: Discussionmentioning

confidence: 99%

Low density lipoprotein - rosiglitazone - chitosan-calcium alginate/nanoparticles inhibition of human tenon's fibroblasts activation and proliferation

et al. 2017

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“…The hydrophilic structure of CS promotes cell adhesion, proliferation, and differentiation of different types of cells and the polycationic nature of CS at a mildly acidic condition allows immobilization of negatively charged enzymes, proteins, and DNA for gene delivery [3,4]. CS for tissue engineering and regenerative medicine could be designed in various forms, such as hydrogels, sponges, fibers, sheets, films, and other structures [5].…”

Section: Introductionmentioning

confidence: 99%

Progress in the Development of Chitosan-Based Biomaterials for Tissue Engineering and Regenerative Medicine

et al. 2019

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Over the last few decades, chitosan has become a good candidate for tissue engineering applications. Derived from chitin, chitosan is a unique natural polysaccharide with outstanding properties in line with excellent biodegradability, biocompatibility, and antimicrobial activity. Due to the presence of free amine groups in its backbone chain, chitosan could be further chemically modified to possess additional functional properties useful for the development of different biomaterials in regenerative medicine. In the current review, we will highlight the progress made in the development of chitosan-containing bioscaffolds, such as gels, sponges, films, and fibers, and their possible applications in tissue repair and regeneration, as well as the use of chitosan as a component for drug delivery applications.

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“…It has been demonstrated that the combined action of bone morphogenetic proteins and scaffold polymers may enhance bone tissue regeneration. A study by Venkatesan et al (2017) [53] proved that CS-based scaffolds have the capacity to systematically and sustainably release BMP-20 and Shu et al [54] showed that the relationship between the osteogenic property of this protein and a 2-N,6-0-sulfated CS may enhance bone tissue development. Duruel et al [20] highlighted the importance of growth factors in the regeneration process: IGF-1 promotes cell recruitment to the affected areas within a few hours, and BMPs are osteoinductive factors expressed in mature bone, which plays a crucial role in the regulation of bone metabolism.…”

Section: Discussionmentioning

confidence: 99%

Nanomaterials for Periodontal Tissue Engineering: Chitosan-Based Scaffolds. A Systematic Review

et al. 2020

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Introduction. Several biomaterials are used in periodontal tissue engineering in order to obtain a three-dimensional scaffold, which could enhance the oral bone regeneration. These novel biomaterials, when placed in the affected area, activate a cascade of events, inducing regenerative cellular responses, and replacing the missing tissue. Natural and synthetic polymers can be used alone or in combination with other biomaterials, growth factors, and stem cells. Natural-based polymer chitosan is widely used in periodontal tissue engineering. It presents biodegradability, biocompatibility, and biological renewability properties. It is bacteriostatic and nontoxic and has hemostatic and mucoadhesive capacity. The aim of this systematic review is to obtain an updated overview of the utilization and effectiveness of chitosan-based scaffold (CS-bs) in the alveolar bone regeneration process. Materials and Methods. During database searching (using PubMed, Cochrane Library, and CINAHL), 72 items were found. The title, abstract, and full text of each study were carefully analyzed and only 22 articles were selected. Thirteen articles were excluded based on their title, five after reading the abstract, twenty-six after reading the full text, and six were not considered because of their publication date (prior to 2010). Quality assessment and data extraction were performed in the twelve included randomized controlled trials. Data concerning cell proliferation and viability (CPV), mineralization level (M), and alkaline phosphatase activity (ALPA) were recorded from each article Results. All the included trials tested CS-bs that were combined with other biomaterials (such as hydroxyapatite, alginate, polylactic-co-glycolic acid, polycaprolactone), growth factors (basic fibroblast growth factor, bone morphogenetic protein) and/or stem cells (periodontal ligament stem cells, human jaw bone marrow-derived mesenchymal stem cells). Values about the proliferation of cementoblasts (CB) and periodontal ligament cells (PDLCs), the activity of alkaline phosphatase, and the mineralization level determined by pure chitosan scaffolds resulted in lower than those caused by chitosan-based scaffolds combined with other molecules and biomaterials. Conclusions. A higher periodontal regenerative potential was recorded in the case of CS-based scaffolds combined with other polymeric biomaterials and bioceramics (bio compared to those provided by CS alone. Furthermore, literature demonstrated that the addition of growth factors and stem cells to CS-based scaffolds might improve the biological properties of chitosan.

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Chitosan as a vehicle for growth factor delivery: Various preparations and their applications in bone tissue regeneration

Cited by 71 publications

References 112 publications

Low density lipoprotein - rosiglitazone - chitosan-calcium alginate/nanoparticles inhibition of human tenon's fibroblasts activation and proliferation

Low density lipoprotein - rosiglitazone - chitosan-calcium alginate/nanoparticles inhibition of human tenon's fibroblasts activation and proliferation

Progress in the Development of Chitosan-Based Biomaterials for Tissue Engineering and Regenerative Medicine

Nanomaterials for Periodontal Tissue Engineering: Chitosan-Based Scaffolds. A Systematic Review

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