Chitosan and silver nanoparticles: Promising anti-toxoplasma agents

Gaafar, Maha R.; Mady, Rasha Fadly; Diab, Radwa G.; Shalaby, Thanaa I.

doi:10.1016/j.exppara.2014.05.005

Cited by 98 publications

(60 citation statements)

References 40 publications

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“…2). Similar effects were reported by Gaafar et al (2014) and El-Zawawy et al (2015). Furthermore, Gaafar et al (2014) supposed that the changes in the shape of the organisms may be secondary to changes resulting from interference of the drugs with DNA synthesis of the parasite or interference with folic acid cycle.…”

Section: Discussionsupporting

confidence: 65%

Effect of Nigella sativa oil on experimental toxoplasmosis

2015

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Toxoplasmosis is a parasitic infection caused by Toxoplasma gondii protozoon. It is most commonly treated by pyrimethamine (PYR); however, this was intolerable by many patients. The aim of this study was to assess therapeutic effects of Nigella sativa oil (NSO) alone and combined with pyrimethamine (PYR) compared to a previous combination of clindamycin (CLN) and (PYR). One hundred Albino mice were used in the current study and were equally divided into five groups: normal (I), infected untreated control (II); infected, treated with NSO-only (III); infected, treated with NSO + PYR (IV); and infected, treated with CLN + PYR (V). The virulent RH Toxoplasma strain was used in infection survival rates estimation, impression smears from liver and spleen, and histopathological and ultrastructural studies were done. Liver malondialdehyde (MDA) level and total antioxidant capacity (TAC) were determined. Interferon-γ and specific IgM were also measured in sera by ELISA. Results showed that NSO alone has no direct anti-Toxoplasma effect, whereas its combination with PYR produced potent effect that is comparable to CLN + PYR. It significantly increased the survival rate and decreased the parasite density and pathological insult in both liver and spleen. Also, significant increase in interferon-γ level denotes stimulation of cellular immunity. NSO + PYR combination markedly improved the antioxidant capacity of Toxoplasma infected mice compared to the infected untreated ones and to CLN/PYR. In conclusion, although NSO, if administered alone, has significant immunostimulant and antioxidant properties, it failed to decrease tachyzoite counts. Combination of NSO and PYR had synergistic effect in treatment of toxoplasmosis.

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Section: Discussionsupporting

confidence: 65%

Effect of Nigella sativa oil on experimental toxoplasmosis

2015

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“…Hybrid nanocomposites are an important platform for the preparation of new materials with advanced properties [1][2][3][4][5][6][7][8]. Noble metal nanostructures encapsulated within or deposited on biopolymers are of particular interest for human [9,10], sensing [11,12], and catalytic applications [13][14][15][16], as these types of materials combine the physicochemical features of noble metal nanostructures and the biological features of polymers [17,18]. In this context, chitosan-based nanocomposites are becoming increasingly important due to their multi-functionality, biocompatibility, biodegradability, wide-ranging pH stability, and activity as bioadsorbents [19][20][21][22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Silver Nanoparticles on Chitosan/Silica Nanofibers: Characterization and Antibacterial Activity

Zienkiewicz-Strzałka

Deryło–Marczewska

Skorik

et al. 2019

IJMS

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A simple, low-cost, and reproducible method for creating materials with even silver nanoparticles (AgNP) dispersion was established. Chitosan nanofibers with silica phase (CS/silica) were synthesized by an electrospinning technique to obtain highly porous 3D nanofiber scaffolds. Silver nanoparticles in the form of a well-dispersed metallic phase were synthesized in an external preparation step and embedded in the CS/silica nanofibers by deposition for obtaining chitosan nanofibers with silica phase decorated by silver nanoparticles (Ag/CS/silica). The antibacterial activity of investigated materials was tested using Gram-positive and Gram-negative bacteria. The results were compared with the properties of the nanocomposite without silver nanoparticles and a colloidal solution of AgNP. The minimum inhibitory concentration (MIC) of obtained AgNP against Staphylococcus aureus (S. aureus) ATCC25923 and Escherichia coli (E. coli) ATCC25922 was determined. The physicochemical characterization of Ag/CS/silica nanofibers using various analytical techniques, as well as the applicability of these techniques in the characterization of this type of nanocomposite, is presented. The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers) were characterized by small angle X-ray scattering (SAXS), X-ray diffraction (XRD), and atomic force microscopy (AFM). The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).

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“…The results show that this matrix has good physical properties and long-time antibacterial activity [18]. The study of Gaafar et al showed that AgNPs used singly or combined with chitosan NPs are promising drugs to eliminate the parasite [19]. However, the poor physical property of chitosan requires a combination of this natural polymer and other synthesized polymers [20,21].…”

Section: Introductionmentioning

confidence: 99%

In Vivo Study of the Antibacterial Chitosan/Polyvinyl Alcohol Loaded with Silver Nanoparticle Hydrogel for Wound Healing Applications

Nguyễn

Nguyen

et al. 2019

International Journal of Polymer Science

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Silver nanoparticles have attracted great interests widely in medicine due to its great characteristics of antibacterial activity. In this research, the antibacterial activity and biocompatibility of a topical gel synthesized from polyvinyl alcohol, chitosan, and silver nanoparticles were studied. Hydrogels with different concentrations of silver nanoparticles (15 ppm, 30 ppm, and 60 ppm) were evaluated to compare their antibacterial activity, nanoparticles’ sizes, and in vivo behaviors. The resulted silver nanoparticles in the hydrogel were characterized by TEM showing the nanoparticles’ sizes less than 22 nm. The in vitro results prove that the antibacterial effects of all of the samples are satisfied. However, the in vivo results demonstrate the significant difference among different hydrogels in wound healing, where hydrogel with 30 ppm shows the best healing rate.

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Chitosan and silver nanoparticles: Promising anti-toxoplasma agents

Cited by 98 publications

References 40 publications

Effect of Nigella sativa oil on experimental toxoplasmosis

Effect of Nigella sativa oil on experimental toxoplasmosis

Silver Nanoparticles on Chitosan/Silica Nanofibers: Characterization and Antibacterial Activity

In Vivo Study of the Antibacterial Chitosan/Polyvinyl Alcohol Loaded with Silver Nanoparticle Hydrogel for Wound Healing Applications

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