Chitosan/aminoacid hydrogels with antimicrobial and bioactive properties as new scaffolds for human mesenchymal stem cells culture applicable in wound healing

Piątkowski, Marek; Kitala, Diana; Radwan-Pragłowska, Julia; Janus, Łukasz; Klama-Baryła, Agnieszka; Łabuś, Wojciech; Tomanek, Ewa; Glik, Justyna; Matýsek, Dalibor; Kawecki, Marek

doi:10.3144/expresspolymlett.2018.8

Cited by 17 publications

(12 citation statements)

References 18 publications

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“…More importantly, FT–IR spectra of all aerogels still showed bands coming from amino groups of various intensity, which suggested that biomaterials are capable of interaction with the cell membrane components of cultured cells. Additionally, bands typical for aliphatic groups, glycosidic bonds, as well as pyranose rings were still present, which confirmed that chitosan did not undergo decomposition during the reaction [ 25 , 26 , 27 , 28 , 29 ].…”

Section: Resultsmentioning

confidence: 82%

“…Scaffolds dedicated to regenerative medicine applications besides high porosity should exhibit good swelling properties, in order to provide an appropriate environment for cells growth [ 24 , 25 , 26 , 27 , 28 , 29 ]. The constant access to culture medium is crucial for cell proliferation.…”

Section: Resultsmentioning

confidence: 99%

“…VK2/E6E7 cells were more resistant to external factors than primary cells. Therefore, the cytotoxic effect of the scaffolds was unexpected, especially as numerous studies confirmed the biocompatibility of the chitosan-based materials [ 27 , 28 , 29 , 30 , 31 ]. It could be observed that the cytotoxicity of the scaffolds was not correlated with their sterility.…”

Section: Resultsmentioning

confidence: 99%

“…It is not very likely, that the crosslinked chitosan was cytotoxic by itself, since the previous study on the L929 cell line and MSC primary cells showed that it was not only non-toxic, but also had a positive impact on cell proliferation [ 25 , 26 , 27 , 28 ]. However, previously described chitosan scaffolds were prepared by using only one or two aminoacids at the time.…”

Section: Resultsmentioning

confidence: 99%

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The Potential of Novel Chitosan-Based Scaffolds in Pelvic Organ Prolapse (POP) Treatment through Tissue Engineering

et al. 2020

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The growing number of female reproductive system disorders creates a need for novel treatment methods. Tissue engineering brings hope for patients, which enables damaged tissue reconstruction. For this purpose, epithelial cells are cultured on three-dimensional scaffolds. One of the most promising materials is chitosan, which is known for its biocompatibility and biodegradability. The aim of the following study was to verify the potential of chitosan-based biomaterials for pelvic organ prolapse regeneration. The scaffolds were obtained under microwave-assisted conditions in crosslinking reactions, using dicarboxylic acids and aminoacid as crosslinkers, including l-glutamic acid, adipic acid, malonic acid, and levulinic acid. The products were characterized over their physicochemical and biological properties. FT–IR analysis confirmed formation of amide bonds. The scaffolds had a highly porous structure, which was confirmed by SEM analysis. Their porosity was above 90%. The biomaterials had excellent swelling abilities and very good antioxidant properties. The cytotoxicity study was performed on vaginal epithelial VK2/E6E7 and human colon cancer HCT116 cell lines. The results showed that after certain modifications, the proposed scaffolds could be used in pelvic organ prolapse (POP) treatment.

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Section: Resultsmentioning

confidence: 82%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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The Potential of Novel Chitosan-Based Scaffolds in Pelvic Organ Prolapse (POP) Treatment through Tissue Engineering

et al. 2020

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“…More compact structures were observed for HCl and PBS swollen samples, although pits and grooves still appeared. Whatever the type of cross-linking, networks formed by cross-linked chitosan usually has been reported to be porous [28][29][30][31][32], showing great resemblance to that of CsFu:BMI 1:1 hydrogel. In the case of study, by the implementation of the Diels-Alder reaction improved microporous structures were achieved for some of the compositions.…”

Section: Resultsmentioning

confidence: 99%

Reversible swelling behaviour of Diels–Alder clicked chitosan hydrogels in response to pH changes

Guaresti¹,

García‐Astrain²,

Urbina³

et al. 2019

Express Polym. Lett.

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Poly(propylene oxide)-poly(ethylene oxide)-poly(propylene oxide) (PPO-PEO-PPO) based bismaleimide (BMI) was employed as cross-linking agent for the synthesis of pH-sensitive clicked hydrogels by Diels-Alder (DA) reaction with furan-grafted chitosan in aqueous solution. The effect of the surrounding pH over the microstructure and the swelling ability of the hydrogels was evaluated depending on the initial composition. The results suggested that the hydrogels maintained the characteristic responsive properties of the original biopolymer even after the cross-linking reaction. The different macromolecular networks remarkably affected the final properties, especially when referring to pH-swelling sensitiveness and hydrogel porosity. In addition, the swelling parameters revealed that the hydrogels presented large liquid absorption capacity, showing excellent recovery properties and responsiveness at different pHs. The promising features of the ensuing hydrogels made them suitable as targeted pH-sensitive drug delivery systems.

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The study of factors affecting the swelling of ultrasound-prepared hydrogel

Ebrahimi

2018

Polym. Bull.

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Chitosan/aminoacid hydrogels with antimicrobial and bioactive properties as new scaffolds for human mesenchymal stem cells culture applicable in wound healing

Cited by 17 publications

References 18 publications

The Potential of Novel Chitosan-Based Scaffolds in Pelvic Organ Prolapse (POP) Treatment through Tissue Engineering

The Potential of Novel Chitosan-Based Scaffolds in Pelvic Organ Prolapse (POP) Treatment through Tissue Engineering

Reversible swelling behaviour of Diels–Alder clicked chitosan hydrogels in response to pH changes

The study of factors affecting the swelling of ultrasound-prepared hydrogel

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