Chitosan‐alginate membranes accelerate wound healing

Caetano, Guilherme Ferreira; Frade, Marco Andrey Cipriani; Andrade, Thiago Antônio Moretti de; Leite, Marcel Nani; Bueno, Cecília Zorzi; Moraes, Ângela Maria; Ribeiro-Paes, João Tadeu

doi:10.1002/jbm.b.33277

Cited by 99 publications

(71 citation statements)

References 44 publications

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“…Color deconvolution ImageJ software was used to evaluate the percentage of blue (collagen) staining in the image area. This software recognizes the colors of the image and decomposes them into three basic colors: blue (collagen), red, and purple, thus measuring the color of blue as the percentage of total collagen pixels in each image (Caetano et al, 2015).…”

Section: Methodsmentioning

confidence: 99%

Effect of photobiomodulation associated with cell therapy in the process of cutaneous regeneration in third degree burns in rats

Andrade

Brassolatti

Luna

et al. 2020

J Tissue Eng Regen Med

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Due to the complexity involved in the healing process of full thickness burns, the literature looks for alternatives to optimize tissue reconstruction. The objective of this study was to explore the action of photobiomodulation therapy associated with MSCs in the healing process of third degree burns. A total of 96 male Wistar rats were used, distributed in four groups with 24 animals each: Control Group, Laser Group, Cell Therapy Group, and Laser Group and Cell Therapy. The burn was performed with aluminum plate (150 C). We performed analysis of wound contraction, histology, immunohistochemistry, birefringence analysis, and immunoenzymatic assay to evaluate tissue quality. Our results demonstrate that the association of the techniques is able to accelerate the repair process, modulating the inflammatory process, presenting a cutaneous tissue with better quality. Thus, we conclude that the use of photobiomodulation therapy associated with cell therapy is a promising treatment in the repair of total thickness burns.

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Section: Methodsmentioning

confidence: 99%

Effect of photobiomodulation associated with cell therapy in the process of cutaneous regeneration in third degree burns in rats

Andrade

Brassolatti

Luna

et al. 2020

J Tissue Eng Regen Med

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“…Colour Deconvolution ImageJ software was used to evaluate the percentage of new bone and connective tissue formation on 50x magnification images. The morphometric analysis, corresponding to red color, was measured as the percentage of the total number of pixels in each original image, as described elsewhere [24].…”

Section: Histologymentioning

confidence: 99%

Cellularized versus decellularized scaffolds for bone regeneration

et al. 2016

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An optimal scaffold based strategy for in vivo repair of large bone defects and its associated problems is presented in this work. Three polymeric scaffolds produced by using an extrusion-based additive manufacturing system were examined in a rat critical bone defect model: scaffolds without cells, with undifferentiated Adipose-derived mesenchymal stem cells (ADSCs) and differentiated ADSCs (osteoblasts). Scaffolds with undifferentiated cells seem to be the best strategy as they exhibited around 22% more bone formation than natural bone healing, and around 15% more than the two other cases.Authors observed that scaffolds enabled cell migration and tissue formation. Results suggest that undifferentiated ADSCs strongly contribute to new bone formation with no rejection if scaffolds are used to support cell migration, proliferation and differentiation. Our long-term goal is to engineer high-quality cell seeded-scaffolds (autograft and allograft) for bone regeneration, mainly in elderly patients.

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“…On the other hand, the microbicidal activity exhibited by Ch is not maintained after PEC formation because of the unavailability of free amino groups to interact with negatively charged molecules on the bacterial surface . Thus, although the resulting biomaterial has no intrinsic antibacterial activity, it works effectively as a physical barrier against bacterial attack and allows gas exchange; it is, therefore, useful for wound dressings …”

Section: Introductionmentioning

confidence: 99%

Polysaccharide‐based membranes loaded with erythromycin for application as wound dressings

Souza

Moraes

2016

J of Applied Polymer Sci

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In this study, the antibiotic erythromycin (Ery) was incorporated into chitosan (Ch)-alginate (A) and Ch-xanthan (X) membranes with the aim of using them as bioactive wound dressings. Drug incorporation was performed by direct addition (DA) to the polysaccharide mixture and by membrane impregnation in solution (IS). A higher incorporation efficiency was obtained for DA, but higher amounts of drug were loaded into membranes by the IS method (maxima % 2.1 and 0.7 g/g for Ch-X and Ch-A, respectively) because the initial concentration of drug could be higher than that in the DA method. Ery release in phosphate-buffered saline was slow, reaching about 12 and 32 mg of drug/g of membrane in 60 h for Ch-X and 4 and 16 mg/g for Ch-A by the DA and IS methods, respectively. With formulations prepared with IS, the required therapeutic dosage was reached within 60 h, whereas for those incorporating the drug by DA, prolonged use would be required. Both membrane types behaved as drug reservoirs, providing continuous antibiotic release to the wound site. Formulations with higher drug contents showed effective antibacterial activity against two species of bacteria commonly found in skin lesions, Staphylococcus aureus and Pseudomonas aeruginosa, and were thus potentially capable of protecting the wound site from bacterial attack.Erythromycin (Ery) is a medium-spectrum antibiotic of the macrolide family and is produced by Streptomyces erythreus. 6 Susceptible microorganisms include aerobic Gram-positive bacteria, Gram-negative cocci, spirochetes, actinomycetes, rickettsia, Chlamydia trachomatis, Mycoplasma pneumoniae, Legionella pneumophila, nontuberculous mycobacteria, and some anaerobic bacteria. 7 This antibiotic consists of a 13-carbon ring with two sugars attached by glycosidic linkages 8,9 (Figure 1) and has a molecular weight of 733.9 Da. It is slightly soluble in water and very soluble in organic solvents such as ethanol 10 ; it is useful in the therapy of pneumonia, diphtheria, whooping cough, urethritis, and erythrasma and acne treatments. 7 For topical administration, the common dosage is 20 mg/g of vehicle. 11The incorporation of Ery into wound dressings is of interest if prolonged protection and drug delivery are simultaneously desired, mostly for extensive and painful lesions of difficult manipulation. These systems show improved therapy efficiency by means of an appropriate release profile; this leads to drug availability at the wound site for long periods and, thereby, reduces the need for repeated administrations. 12The main purpose of using a wound dressing is to provide a suitable environment at the wound site so that healing is fast

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Chitosan‐alginate membranes accelerate wound healing

Cited by 99 publications

References 44 publications

Effect of photobiomodulation associated with cell therapy in the process of cutaneous regeneration in third degree burns in rats

Effect of photobiomodulation associated with cell therapy in the process of cutaneous regeneration in third degree burns in rats

Cellularized versus decellularized scaffolds for bone regeneration

Polysaccharide‐based membranes loaded with erythromycin for application as wound dressings

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