Chitosan-adjuvanted Salmonella subunit nanoparticle vaccine for poultry delivered through drinking water and feed

Sankar, Raman; Han, Yi; Dhakal, Santosh; Lakshmanappa, Yashavanth Shaan; Ghimire, Shristi; Feliciano-Ruiz, Ninoshkaly; Senapati, Sujata; Narasimhan, Balaji; Selvaraj, Ramesh K.; Renukaradhya, Gourapura J.

doi:10.1016/j.carbpol.2020.116434

Cited by 40 publications

(47 citation statements)

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“…Delivery of the vaccine through oral gavage, drinking water, and feed increased the antigen specific IgY and IgA antibody response following the optimized dose of Salmonella challenge (5 × 10 6 CFU) infection. Oral gavage and drinking water delivered nanovaccine immunization induced immune response correlated with reduction in Salmonella load in cecum (Renu et al, 2020b). Both our study outcomes confirmed that flagellin protein surface conjugation of CS NPs is necessary to improve the efficacy of oral Salmonella vaccine in layer chickens; two doses of nanovaccine inoculation is sufficient and the third dose did not further boost the antibody response; and the optimized bacterial challenge dose is crucial to appreciate the vaccine induced protective efficacy.…”

Section: Cs Nps Based Vaccines For Bacterial Infectious Diseasesupporting

confidence: 68%

“…Although due to high bacterial challenge dose [1 × 10 9 colony-forming unit (CFU) per bird] used in that nanovaccine trial we did not detect substantial reduction in the bacterial colonization, but our this first study confirmed that surface flagellin protein coating on CS NPs is necessary to reach the vaccine to ileum immune cells of layer chickens when delivered orally (Renu et al, 2020c). To determine the protective efficacy of nanovaccine, in another study (Renu et al, 2020b), we optimized CS NPs by altering the concentrations of chitosan and TPP ratios used in the vaccine formulation as well as experimental conditions. The optimized CS NPs surface modified with flagellin protein delivered orally in chickens reached PP's and ileum, and in vitro treatment of the nanovaccine in chicken immune cells increased the TLRs 1, 2, 3, 4, 5, 7, 15, and 21, and IFN-γ, IL-2, IL-4, and IL-10 gene expression.…”

Section: Cs Nps Based Vaccines For Bacterial Infectious Diseasementioning

confidence: 87%

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Chitosan Nanoparticle Based Mucosal Vaccines Delivered Against Infectious Diseases of Poultry and Pigs

Renu

Renukaradhya

2020

Front. Bioeng. Biotechnol.

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Section: Cs Nps Based Vaccines For Bacterial Infectious Diseasesupporting

confidence: 68%

Section: Cs Nps Based Vaccines For Bacterial Infectious Diseasementioning

confidence: 87%

Chitosan Nanoparticle Based Mucosal Vaccines Delivered Against Infectious Diseases of Poultry and Pigs

Renu

Renukaradhya

2020

Front. Bioeng. Biotechnol.

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“…The gastrointestinal tract is the predilection site for colonization of Salmonella , and it augments local mucosal IgA secretion and prevents bacterial attachment to the epithelial cells [ 21 ]. In our previous studies in layers and broilers, CS(OMP+FLA)-F vaccine oral inoculation elicited robust specific local IgA antibody rather than IgG antibody responses and provided partial protection against Salmonella colonization [ 5 , 6 , 15 ]. A study in mice showed that mannosylated chitosan microsphere-based vaccine delivered orally binds to mannose receptors on macrophages and triggers the IgA antibody response [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

“…Outer membrane proteins (OMP) and flagellin proteins (FLA) were isolated as previously described [ 14 ]. The vaccine formulations were prepared by an ionic gelation method and characterized as previously described [ 6 , 15 ]. The mannose-modified CS(OMP+FLA)-M and CS(OMP+FLA)-F&M vaccines were prepared as reported earlier [ 16 , 17 ], with few modifications.…”

Section: Methodsmentioning

confidence: 99%

“…This outcome was associated with the secretion of increased antigen-specific mucosal and systemic antibodies, splenocytes proliferation, and the frequency of IFNγ-producing T-cell responses. A similar study in layer chickens with CS(OMP+FLA)-F vaccine delivered orally targeted intestinal immune sites and induced mucosal antibody and cell-mediated immune responses, resulting in reduced challenge SE load [ 6 ]. Additionally, CS(OMP+FLA)-F-vaccine-treated chicken immune cells showed enhancement of various Toll-like receptors (TLRs) and Th1 and Th2 cytokine gene expression [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Mannose-Modified Chitosan-Nanoparticle-Based Salmonella Subunit OralVaccine-Induced Immune Response and Efficacy in a Challenge Trial in Broilers

et al. 2020

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Controlling Salmonella enterica serovar Enteritidis (SE) infection in broilers is a huge challenge. In this study, our objective was to improve the efficacy of a chitosan nanoparticle (CS)-based Salmonella subunit vaccine for SE, containing immunogenic outer membrane proteins (OMP) and flagellin (FLA), called the CS(OMP+FLA) vaccine, by surface conjugating it with mannose to target dendritic cells, and comparing the immune responses and efficacy with a commercial live Salmonella vaccine in broilers. The CS(OMP+FLA)-based vaccines were administered orally at age 3 days and as a booster dose after three weeks, and the broilers were challenged with SE at 5 weeks of age. Birds were sacrificed 10 days post-challenge and it was observed that CS(OMP+FLA) vaccine surface conjugated with both mannose and FLA produced the greatest SE reduction, by over 1 log10 colony forming unit per gram of the cecal content, which was comparable to a commercial live vaccine. Immunologically, specific mucosal antibody responses were enhanced by FLA-surface-coated CS(OMP+FLA) vaccine, and mannose-bound CS(OMP+FLA) improved the cellular immune response. In addition, increased mRNA expression of Toll-like receptors and cytokine was observed in CS(OMP+FLA)-based-vaccinated birds. The commercial live vaccine failed to induce any such substantial immune response, except that they had a slightly improved T helper cell frequency. Our data suggest that FLA-coated and mannose-modified CS(OMP+FLA) vaccine induced robust innate and adaptive cell-mediated immune responses and substantially reduced the Salmonella load in the intestines of broilers.

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Advances of Nanotechnology Toward Vaccine Development Against Animal Infectious Diseases

Wang

Gao

Wang

et al. 2023

Adv Funct Materials

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Vaccines are the most effective means of controlling infectious diseases. Increasingly epidemics have driven the development of vaccines for human use. However, insufficient attention has been paid to preventing animal epidemics. The spread of animal epidemics directly affects food and nutritional safety, the economy, and outbreaks of zoonotic diseases, which can eventually threaten human health. Reducing safety risks, saving production costs, adapting to mass vaccination, and improving immunogenicity are important that must be considered in animal vaccines. Nanotechnology in vaccine development provides unique advantages in meeting these challenges, both at in vitro preparation and in vivo immune response levels. In terms of in vitro vaccine formulation, nanoparticles offer the possibility to provide multiple antigen display sites, maintain antigen conformation, and improve vaccine stability, which facilitates the development of vaccines with enhanced immunogenicity and thermal stability. In terms of in vivo vaccine‐induced immunity, nanoparticles show superior immunostimulatory activity which facilitates multiple immune response processes, including antigen delivery, cellular uptake, antigen presentation, and lymphocyte activation, thus markedly augmenting vaccine‐mediated immune responses. Herein, the application of nanotechnology to vaccine development and the characteristics of animal vaccines are reviewed, aiming to provide general guidelines for the design of future vaccines against animal infectious diseases and promote harmonious coexistence of humans and animals.

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Chitosan-adjuvanted Salmonella subunit nanoparticle vaccine for poultry delivered through drinking water and feed

Cited by 40 publications

References 50 publications

Chitosan Nanoparticle Based Mucosal Vaccines Delivered Against Infectious Diseases of Poultry and Pigs

Chitosan Nanoparticle Based Mucosal Vaccines Delivered Against Infectious Diseases of Poultry and Pigs

Mannose-Modified Chitosan-Nanoparticle-Based Salmonella Subunit OralVaccine-Induced Immune Response and Efficacy in a Challenge Trial in Broilers

Advances of Nanotechnology Toward Vaccine Development Against Animal Infectious Diseases

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