Chitosan 3D cell culture system promotes naïve-like features of human induced pluripotent stem cells: A novel tool to sustain pluripotency and facilitate differentiation

Chang, Po-Hsiang; Chao, Hsiao-Mei; Chern, Edward; Hsu, Shan‐hui

doi:10.1016/j.biomaterials.2020.120575

Cited by 50 publications

(34 citation statements)

References 89 publications

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“…This shows that the fiber surface is a natural material, which indirectly proves that the prepared spinning has a core–shell double-layer structure. Chang [ 47 ] et al reported that the membranes prepared by hydrophilic materials reduced the contact angle and the membrane-containing HA had the smallest contact angle. In our study, A2 (HA) had the smallest contact angle.…”

Section: Resultsmentioning

confidence: 99%

Coaxial Electrospun PLLA Fibers Modified with Water-Soluble Materials for Oligodendrocyte Myelination

et al. 2021

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Myelin sheaths are essential in maintaining the integrity of axons. Development of the platform for in vitro myelination would be especially useful for demyelinating disease modeling and drug screening. In this study, a fiber scaffold with a core–shell structure was prepared in one step by the coaxial electrospinning method. A high-molecular-weight polymer poly-L-lactic acid (PLLA) was used as the core, while the shell was a natural polymer material such as hyaluronic acid (HA), sodium alginate (SA), or chitosan (CS). The morphology, differential scanning calorimetry (DSC), Fourier transform infrared spectra (FTIR), contact angle, viability assay, and in vitro myelination by oligodendrocytes were characterized. The results showed that such fibers are bead-free and continuous, with an average size from 294 ± 53 to 390 ± 54 nm. The DSC and FTIR curves indicated no changes in the phase state of coaxial brackets. Hyaluronic acid/PLLA coaxial fibers had the minimum contact angle (53.1° ± 0.24°). Myelin sheaths were wrapped around a coaxial electrospun scaffold modified with water-soluble materials after a 14-day incubation. All results suggest that such a scaffold prepared by coaxial electrospinning potentially provides a novel platform for oligodendrocyte myelination.

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Section: Resultsmentioning

confidence: 99%

Coaxial Electrospun PLLA Fibers Modified with Water-Soluble Materials for Oligodendrocyte Myelination

et al. 2021

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“…The above findings included optimization of hPSC lines and directed differentiation of hPSCs into desired lineages, as well as modeling hematopoietic disorders using patient-derived iPSCs. Along with the findings presented at this meeting, multiple efforts have been made to acquire naive hPSCs (Chang et al, 2021;Giulitti et al, 2019), and patient-derived iPSCs have been created to model many hematologic disorders such as MDS and other myeloid leukemias (Chao et al, 2017;Wang et al, 2021;Wesely et al, 2020). These approaches will allow us to model and discover human biology and provide valuable insights for potential translational therapies in human cells and patients.…”

Section: Lineage Tracing and Clonal Dynamics Of Hematopoiesismentioning

confidence: 97%

From development toward therapeutics, a collaborative effort on blood progenitors

2021

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“…Collectively, chitosan membranes not only promoted the naïve pluripotent features of hiPSCs but also provided a novel 3D differentiation platform. [24] 2.6. Olfactory Ensheathing Cells (OECs)…”

Section: Induced Pluripotent Stem Cells (Ipscs)mentioning

confidence: 99%

Trends of Chitosan Based Delivery Systems in Neuroregeneration and Functional Recovery in Spinal Cord Injuries

et al. 2021

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Spinal cord injury (SCI) is one of the most complicated nervous system injuries with challenging treatment and recovery. Regenerative biomaterials such as chitosan are being reported for their wide use in filling the cavities, deliver curative drugs, and also provide adsorption sites for transplanted stem cells. Biomaterial scaffolds utilizing chitosan have shown certain therapeutic effects on spinal cord injury repair with some limitations. Chitosan-based delivery in stem cell transplantation is another strategy that has shown decent success. Stem cells can be directed to differentiate into neurons or glia in vitro. Stem cell-based therapy, biopolymer chitosan delivery strategies, and scaffold-based therapeutic strategies have been advancing as a combinatorial approach for spinal cord injury repair. In this review, we summarize the recent progress in the treatment strategies of SCI due to the use of bioactivity of chitosan-based drug delivery systems. An emphasis on the role of chitosan in neural regeneration has also been highlighted.

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Chitosan 3D cell culture system promotes naïve-like features of human induced pluripotent stem cells: A novel tool to sustain pluripotency and facilitate differentiation

Cited by 50 publications

References 89 publications

Coaxial Electrospun PLLA Fibers Modified with Water-Soluble Materials for Oligodendrocyte Myelination

Coaxial Electrospun PLLA Fibers Modified with Water-Soluble Materials for Oligodendrocyte Myelination

From development toward therapeutics, a collaborative effort on blood progenitors

Trends of Chitosan Based Delivery Systems in Neuroregeneration and Functional Recovery in Spinal Cord Injuries

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