Chitinous Structures as Potential Targets for Insect Pest Control

Tetreau, Guillaume; Wang, Ping

doi:10.1007/978-981-13-7318-3_13

Cited by 15 publications

(7 citation statements)

References 128 publications

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“…All insects produce exoskeletons, which are essential in all developmental stages to protect the insect from mechanical injury and predation. Due to growth and development, the old exoskeleton becomes a physical constraint, and the insect synthesizes a new cuticle and partially digests and sheds the cuticle from the previous developmental stage during each molt [ 48 – 51 ]. It has been observed that the growth of insects is highly dependent on their capability for remodeling of the exoskeleton.…”

Section: Discussionmentioning

confidence: 99%

Whole-transcriptome profiling across different developmental stages of Aedes albopictus (Diptera: Culicidae) provides insights into chitin-related non-coding RNA and competing endogenous RNA networks

Liu

Peng

et al. 2023

Parasites Vectors

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Background The Asian tiger mosquito, Aedes albopictus, is one of the most invasive species and a vector of numerous arboviruses. The deleterious effects of long-term and inappropriate use of chemical pesticides have stimulated the exploration of new, environmentally friendly control strategies. Non-coding RNAs (ncRNAs) have been proven to participate in almost all biological processes of insects. Methods In this study, circular RNAs (circRNAs) and microRNAs (miRNAs) covering five developmental stages [egg, early larvae, late larvae, pupae, adult (female and male)] of A. albopictus were obtained using whole-transcriptome sequencing technology. Combined with long non-coding RNAs (lncRNAs) from previous research, circRNA/lncRNA‒miRNA‒mitochondrial RNA (mRNA) networks were constructed. Results A total of 1434 circRNAs and 208 miRNAs were identified. More differentially expressed circRNAs (DE circRNAs) and miRNAs (DE miRNAs) were found in the egg versus early larvae comparison group. Functional enrichment analysis demonstrated that most of the circRNA/lncRNA‒miRNA‒mRNA networks were involved in chitin metabolism. Hub genes of each circRNA/lncRNA‒miRNA‒mRNA network were screened out, which can be used as novel targets to disturb the molting process of A. albopictus. Conclusions Regulatory relationships obtained from competing endogenous RNA (ceRNA) networks provide more information to manipulate the metamorphosis process and are helpful for developing effective and sustainable methods to control mosquitoes. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

Whole-transcriptome profiling across different developmental stages of Aedes albopictus (Diptera: Culicidae) provides insights into chitin-related non-coding RNA and competing endogenous RNA networks

Liu

Peng

et al. 2023

Parasites Vectors

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“…Caligus rogercresseyi is the most important ectoparasite in Chilean salmon farming; thereby, interest in developing new control methods has encouraged the study of their physiology and molecular mechanisms of interaction with the host. Peritrophins, as the main component of the peritrophic membrane, have been evaluated as a pharmacological target for insect control methods [ 63 , 64 ]. Furthermore, peritrophin proteins have been related in some insects to the protective response against damage caused by toxic compounds, such as insecticides [ 14 , 65 ].…”

Section: Discussionmentioning

confidence: 99%

Peritrophin-like Genes Are Associated with Delousing Drug Response and Sensitivity in the Sea Louse Caligus rogercresseyi

Casuso

Núñez‐Acuña

Valenzuela-Muñoz

et al. 2022

IJMS

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Caligus rogercresseyi is the main ectoparasite that affects the salmon industry in Chile. The mechanisms used by the parasite to support its life strategy are of great interest for developing control strategies. Due to the critical role of insect peritrophins in host–parasite interactions and response to pest control drugs, this study aimed to identify and characterize the peritrophin-like genes present in C. rogercresseyi. Moreover, the expression of peritrophin-like genes was evaluated on parasites exposed to delousing drugs such as pyrethroids and azamethiphos. Peritrophin genes were identified by homology analysis among the sea louse transcriptome database and arthropods peritrophin-protein database obtained from GenBank and UniProt. Moreover, the gene loci in the parasite genome were located. Furthermore, peritrophin gene expression levels were evaluated by RNA-Seq analysis in sea louse developmental stages and sea lice exposed to delousing drugs deltamethrin, cypermethrin, and azamethiphos. Seven putative peritrophin-like genes were identified in C. rogercresseyi with high homology with other crustacean peritrophins. Differences in the presence of signal peptides, the number of chitin-binding domains, and the position of conserved cysteines were found. In addition, seven peritrophin-like gene sequences were identified in the C. rogercresseyi genome. Gene expression analysis revealed a stage-dependent expression profile. Notably, differential regulation of peritrophin genes in resistant and susceptible populations to delousing drugs was found. These data are the first report and characterization of peritrophin genes in the sea louse C. rogercresseyi, representing valuable knowledge to understand sea louse biology. Moreover, this study provides evidence for a deeper understanding of the molecular basis of C. rogercresseyi response to delousing drugs.

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“…This result is consistent with expanded genes in S. cinerearia, the genes involved in biosynthesis of cutin not only expanded but also under positively selection in Geometridae. Chitinous structures are physiologically fundamental in insects, and are target sites for the development of new insect-pest-control strategies [28][29][30]. Further studying on this gene may help us develop new means of pest control.…”

Section: Positively Selected Genes In Geometridaementioning

confidence: 99%

A chromosome-level genome of Semiothisa cinerearia provides insights into its genome evolution and control

Chi

Wang

et al. 2022

BMC Genomics

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Background Semiothisa cinerearia belongs to Geometridae, which is one of the most species-rich families of lepidopteran insects. It is also one of the most economically significant pests of the Chinese scholar tree (Sophora japonica L.), which is an important urban greenbelt trees in China due to its high ornamental value. A genome assembly of S. cinerearia would facilitate study of the control and evolution of this species. Results We present a reference genome for S. cinerearia; the size of the genome was ~ 580.89 Mb, and it contained 31 chromosomes. Approximately 43.52% of the sequences in the genome were repeat sequences, and 21,377 protein-coding genes were predicted. Some important gene families involved in the detoxification of pesticides (P450) have expanded in S. cinerearia. Cytochrome P450 gene family members play key roles in mediating relationships between plants and insects, and they are important in plant secondary metabolite detoxification and host-plant selection. Using comparative analysis methods, we find positively selected gene, Sox15 and TipE, which may play important roles during the larval-pupal metamorphosis development of S. cinerearia. Conclusion This assembly provides a new genomic resource that will aid future comparative genomic studies of Geometridae species and facilitate future evolutionary studies on the S. cinerearia.

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Chitinous Structures as Potential Targets for Insect Pest Control

Cited by 15 publications

References 128 publications

Whole-transcriptome profiling across different developmental stages of Aedes albopictus (Diptera: Culicidae) provides insights into chitin-related non-coding RNA and competing endogenous RNA networks

Whole-transcriptome profiling across different developmental stages of Aedes albopictus (Diptera: Culicidae) provides insights into chitin-related non-coding RNA and competing endogenous RNA networks

Peritrophin-like Genes Are Associated with Delousing Drug Response and Sensitivity in the Sea Louse Caligus rogercresseyi

A chromosome-level genome of Semiothisa cinerearia provides insights into its genome evolution and control

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