Chitinase production by <i>Streptomyces viridificans:</i> its potential in fungal cell wall lysis

Gupta, Rani; Saxena, Rajendra Kumar; Chaturvedi, Priya; Virdi, Jugsharan Singh

doi:10.1111/j.1365-2672.1995.tb03421.x

Cited by 194 publications

(119 citation statements)

References 25 publications

(28 reference statements)

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“…Chitinolysis is a very common feature in actinomycetes isolated from soil (Gupta et al 1995), although studies on tropical soils are scarce (Gomes et al 1999). In the present investigation, determined chitinase activity at pH 7·5 and 50°C of concentrated crude enzymes from the studied strains is presented in Table 1.…”

Section: Resultsmentioning

confidence: 99%

“…A liquid chitin medium (Hsu and Lockwood 1975), containing colloidal chitin plus fungal mycelia from Aspergillus parasiticus (1 : 3) (Gupta et al 1995), was used for preparation of a crude extract. After growth of each strain (28°C/7 d/250 rev min −1 ), supernatants were filtered (Whatman no.…”

Section: Antifungal Assaymentioning

confidence: 99%

“…One possible explanation for its inhibition is the action of chitinases and b-glucanases on chitin or glucan present in these fungal cell walls, acting as protective agents (Inbar and Chet 1991). Actinomycetes, in particular the genus Streptomyces, which are Gram-positive mycelial bacteria, ubiquitous in soil, are well known as producers of many extracellular enzymes with polymer-degrading properties, including chitinases (Gupta et al 1995).…”

Section: Introductionmentioning

confidence: 99%

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Chitinolytic activity of actinomycetes from a cerrado soil and their potential in biocontrol

Gomes

Semêdo

Soares

et al. 2000

Lett Appl Microbiol

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R.C. GOMES, L.T.A.S. SEME(r)DO, R.M.A. SOARES, C.S. ALVIANO, L.F. LINHARES and R.R.R. COELHO.2000.The crude enzyme extracts from five actinomycetes selected from a cerrado soil presented very good endochitinolytic activity when compared to a commercial chitinase. Exochitinase and chitobiase activities were also detected. They were identified as Streptomyces, but could not be characterized to species level, probably corresponding to new ones. The crude extracts, obtained from growth on fungal mycelium plus chitin of three of the strains, have shown a very pronounced activity against phytopathogenic fungi. In tests using growing cells, all five strains were active. These data suggest that these strains are potential biocontrol agents.

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Section: Resultsmentioning

confidence: 99%

Section: Antifungal Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chitinolytic activity of actinomycetes from a cerrado soil and their potential in biocontrol

Gomes

Semêdo

Soares

et al. 2000

Lett Appl Microbiol

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“…The homogenate was centrifuged at 16,000 × g for 20 min at 4°C and the supernatant were collected as a crude enzyme extract. CHI activity was measured as described as in [9] with some modifications. An aliquot (1mL) of crude extract was mixed with 0.2 g colloidal chitin (Sigma) and 2 mL of 0.1 M citrate buffer (pH 6.0), then incubated at 37°C for 1 h. The sample was added with 0.5 mL 3,5-dinitrosalicylic acid (DNS solution) and boiled for 15 min.…”

Section: Plant Defense Enzymesmentioning

confidence: 99%

Eliciting Plant Defense on Anthracnose Disease in Chili (Capsicum annuum Linn.) by Sodium Nitroprusside Solution

Thuong¹,

Jitareerat²,

Uthairatanakij³

2015

JFNS

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Abstract:The study on the induction of plant defense against to anthracnose disease of chili by sodium nitroprusside solution was investigated during pre-and post-harvest. Pre-harvest treatment was conducted by spraying the chili plants with sodium nitroprusside (SNP, nitric oxide donor) solution at 0 (control), 0.05 and 0.1 mM every 3 days for 9 times before inoculating with the spore suspension of Colletotrichum gloeosporioides (the causal agent of anthracnose disease) and then sprayed with SNP again 1 time after inoculating. The results revealed that 0.1 mM SNP was the best concentration to suppress anthracnose disease by reducing disease incidence and disease index on chili leaves, and showing the lowest loss of yield caused by anthracnose disease. This founding also showed that either pathogenic inoculation or SNP treatment had ability to induce the activities of peroxidase (POD) and phenylalanine ammonia lyase (PAL) of chili leaves, particularly the chili plant inoculated with pathogenic fungi followed by treated with 0.1 mM SNP showed the highest POD and PAL activities. Postharvest treatment was done by inoculating the mature chilli fruit with C. capcisi for 4 h and followed by dipping in 0 (control) and 4 mM SNP. All fruit samples were kept at 13°C for 15 days. The results showed that chili fruit dipped with 4 mM SNP reduced disease incidence, disease index, delayed weight loss, respiration rate, ethylene production, and also maintained the changes of fruit color (∆E) better than non-treated fruit (control). The activities of chitinase (CHI) and β-1,3-glucanase )GLU( in chili fruit were induced by SNP with the highest peak on day 6 of storage but they were not significant different with the control fruit. These results indicated that SNP might potential leading to the reduction of anthracnose disease in chili by eliciting plant defense related enzymes in both pre-and post-harvest periods.

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“…Streptomyces species are heterotrophic feeders, which can utilize both complex and simple molecules as nutrients. In addition to antibiotics, Streptomyces species liberate several extra cellular enzymes [17]. They produce variety of extra cellular proteases that have been related to aerial mycelium formation and sporulation [18].…”

Section: Introductionmentioning

confidence: 99%

Effects of Cultural Conditions on the Production of Extracellular Protease by Streptomyces albolongus and Streptomyces aburaviensis

N¹

2013

Enz Eng

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Proteolytic activity of two isolates of actinomycetes, Streptomyces albolongus and Streptomyces aburaviences was investigated on the basis of their ability to hydrolyze skimmed milk casein, egg albumin and gelatin. Both isolates were found to have potential for extracellular proteases production. Effects of culture conditions for the production of extracellular protease from S. albolongus and S. aburaviensis were determined. Highest protease yield from S. albolongus was obtained after 5 days of incubation with an initial p H 7, at static state when inoculated in medium composed of 1% glucose, 2% beef extract, 0.2% yeast extract, 0.1% KH 2 PO 4 , 0.3% K 2 HPO 4 , and trace MgSO 4 .7H 2 O. Optimum incubation conditions for S. abureviences were 4 days, with an initial medium p H 8 at shaking condition (100 rpm). S. aburaviences preferred 1.5% lactose and 1.5% tryptone as a carbon and nitrogen source. Both the isolates showed maximum protease yield at 37°C. The result of the present study might be helpful for largescale production of extracellular protease from these Streptomyces spp.

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Chitinase production by Streptomyces viridificans: its potential in fungal cell wall lysis

Cited by 194 publications

References 25 publications

Chitinolytic activity of actinomycetes from a cerrado soil and their potential in biocontrol

Chitinolytic activity of actinomycetes from a cerrado soil and their potential in biocontrol

Eliciting Plant Defense on Anthracnose Disease in Chili (Capsicum annuum Linn.) by Sodium Nitroprusside Solution

Effects of Cultural Conditions on the Production of Extracellular Protease by Streptomyces albolongus and Streptomyces aburaviensis

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